Art. -Nr.: HY-124617A

AMXT-1501 tetrahydrochloride

11 Cited Publications

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Based on 11 publication(s) in Google Scholar

AMXT-1501 tetrahydrochloride is a Bacterial agent and polyamine transport system inhibitor. AMXT-1501 tetrahydrochloride targets membrane phospholipids and exhibits antibacterial activity against a variety of Gram-positive and Gram-negative multidrug-resistant bacteria. AMXT-1501 tetrahydrochloride inhibits capsular biosynthesis in Streptococcus pneumoniae. AMXT-1501 tetrahydrochloride targets ornithine decarboxylase and polyamines to inhibit the proliferation of neuroblastoma cells. AMXT-1501 tetrahydrochloride in combination with DFMO (HY-B0744) induces Apoptosis in neuroblastoma cells. AMXT-1501 tetrahydrochloride is applicable to research related to multidrug-resistant bacterial infections, pneumococcal infections, Streptococcus pneumoniae infections, and neuroblastoma.

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Reinheit: 98.0%
CAS. Nr.: 2444815-84-1
Formel: C₃₂H₇₂Cl₄N₆O₂
Molecular Weight:714.77

Speicherung:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

AMXT-1501 tetrahydrochloride Chemical Structure

CAS. Nr.: 2444815-84-1

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (11)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

Polyamine transport^[1]

In Vitro

AMXT-1501 (1.56-50 μM) tetrahydrochloride exhibits dose-dependent antibacterial activity against a variety of Gram-positive and Gram-negative multidrug-resistant (MDR) bacterial isolates, with MIC₅₀/MIC₉₀ values ranging from 3.13 to 12.5 μM^[1].
AMXT-1501 (8× MIC; 0-24 h) tetrahydrochloride exhibits rapid and potent bactericidal activity against planktonic MRSA, ESBL-producing E. coli, and CR E. coli^[1].
AMXT-1501 (1-4× MIC; 0.5-24 h) tetrahydrochloride reduces biofilm formation in a dose-dependent manner in most tested Staphylococcus aureus (MSSA and MRSA) and Enterococcus faecalis strains, causes severe damage to the cell membranes of MRSA and CR E. coli, increases membrane permeability and depolarizes them, thereby leading to bacterial cell death^[1].
AMXT-1501 (3.125-50 μg/mL; 90 s) tetrahydrochloride binds directly to the bacterial membrane phospholipids CL and PG^[1].
AMXT-1501 (7.4 μM; cultured until OD₆₀₀ reaches 0.2-0.5) tetrahydrochloride inhibits capsular polysaccharide biosynthesis by 61% in Streptococcus pneumoniae serotype 2 (strain D39), depletes intracellular polyamines and glucuronic acid, and simultaneously increases intracellular glucose levels^[2].
AMXT-1501 (7.4 μM) tetrahydrochloride regulates gene expression in Streptococcus pneumoniae D39, thereby enhancing the biosynthesis of polyamines and glucose, inhibiting ATP production and fatty acid synthesis, and upregulating stress response pathways^[3].
AMXT-1501 (0.39-50 μM; 48 h) tetrahydrochloride potently inhibits the viability of human neuroblastoma cell lines BE (2)-C, SMS-KCNR and SH-SY5Y in vitro, with IC₅₀ values of 17.69 μM, 17.72 μM and 14.13 μM, respectively^[4].
AMXT-1501 (2.5 μM; 96 h) tetrahydrochloride induced apoptosis in BE (2)-C, SMS-KCNR and SH-SY5Y human neuroblastoma cells when used in combination with DFMO^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[4]

Cell Line:

BE(2)-C, SMS-KCNR, SH-SY5Y
Concentration:

0.39 µM, 1 µM, 3.1 µM, 10 µM, 31 µM, 50 µM
Incubation Time:

48 h
Result:

AMXT-1501 tetrahydrochloride exhibited cytotoxicity against this panel of NB cell lines.

Apoptosis Analysis^[4]

Cell Line:

BE(2)‐C, SMS‐KCNR and SH‐SY5Y cells
Concentration:

2.5 μM+2.5 mM DFMO
Incubation Time:

96 h
Result:

Induced apoptosis.
Reduced the amount of uncleaved PARP and increased the amounts of cleaved PARP and cleaved caspase-3.

In Vivo

AMXT-1501 (20 mg/kg; i.p.; every 12 h; 3 days) tetrahydrochloride significantly reduces the size of skin abscesses and decreases MRSA loads in BALB/c mice^[1].
AMXT-1501 (20 mg/kg; i.p.; every 12 h; 3 days) tetrahydrochloride significantly improves the survival rate of BALB/c mice with bacterial abdominal infection and reduces the bacterial load of CRE E. coli in their lung and liver tissues^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c (female, 6-8-week-old, 18-22 g) (S. aureus YUSA145 (MRSA)(1×107 CFU))^[1]
Dosage:

20 mg/kg
Administration:

i.p.; every 12 h; 3 days
Result:

Reduced skin abscess areas significantly.
Lowered bacterial loads in abscess tissue (log₁₀ CFU/g) significantly.

Animal Model:

BALB/c (female, 6-8-week-old, 18-22 g) (S. aureus YUSA145 (MRSA)(1×107 CFU))^[1]
Dosage:

20 mg/kg
Administration:

i.p.; every 12 h; 3 days
Result:

Achieved a 50% survival rate through day 7, which was higher than the survival rate in tigecycline-treated mice.
Lowered bacterial loads in lung tissue (log₁₀ CFU/g) significantly.
Lowered bacterial loads in liver tissue (log₁₀ CFU/g) significantly.

Chemical Information

CAS. Nr. 2444815-84-1
Appearance Solid
Molecular Weight 714.77
Formel C₃₂H₇₂Cl₄N₆O₂
Color Off-white to light yellow
SMILES

CCCCCCCCCCCCCCCC(NCCCC[C@@H](N)C(NCCCNCCCCNCCCN)=O)=O.Cl.Cl.Cl.Cl
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (11)

All Cell Proliferation/Viability AssayCell Imaging/StainingWB2D/3D Cell Culture and Differentiation

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Journal Impact Factor

Immunity

Polyamines regulate adaptive antitumor immunity by functional specialization of regulatory T cells. [Abstract]

2025 Aug 12;58(8):2019-2034.e11. PMID: 40749666
Nat Immunol

Epigenetic reprogramming of T cell metabolism restores function and enhances anti-tumor immunity in lung cancer. [Abstract]

2026 Jun;27(6):1268-1281. PMID: 42120791
Nat Commun

Polyamine-mediated ferroptosis amplification acts as a targetable vulnerability in cancer. [Abstract]

2024 Mar 19;15(1):2461. PMID: 38504107
Cell Death Differ

Anaplastic Lymphoma Kinase signaling stabilizes SLC3A2 expression via MARCH11 to promote neuroblastoma cell growth. [Abstract]

2024 Jul;31(7):910-923. PMID: 38858548

AMXT-1501 tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2024 Jul;31(7):910-923. [Abstract]

IC50s of AMXT-1501 (4, 8, 12, 16, 20, 24 μM) in CLB-BAR, CLB-GE, SK-N-BE(2) and SH-SY5Y cells after 5 d treatment.

AMXT-1501 tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2024 Jul;31(7):910-923. [Abstract]

Immunoblot analysis of neuronal differentiation markers (RET and DLG2) in SK-N-BE(2) and SH-SY5Y cells treated with AMXT-1501 (0 or 8 µM) and RA (0 or 5 µM) for 48 h or 24 h .

AMXT-1501 tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2024 Jul;31(7):910-923. [Abstract]

Cells after AMXT-1501 (0 or 8 µM) and RA (0 or 5 µM) treatment as indicated for 48 h or 24 h.
Neuro Oncol

Polyamine acetylation mediates crosstalk between cancer cells and myeloid cells to promote mesenchymal/plurimetabolic states in glioblastoma. [Abstract]

2025 Oct 1;27(10):2574-2591. PMID: 40424600
Emerg Microbes Infect

AMXT-1501 targets membrane phospholipids against Gram-positive and -negative multidrug-resistant bacteria. [Abstract]

2024 Dec;13(1):2321981. PMID: 38422452
Icahn School of Medicine at Mount Sinai

2025.
The University of Texas at San Antonio

2025.
bioRxiv

2025 January 26.
bioRxiv

Genetically encoded fluorescent reporter for polyamines. [Abstract]

2024 Nov 17:2024.08.24.609500. PMID: 39253442

AMXT-1501 tetrahydrochloride purchased from MedChemExpress. Usage Cited in: bioRxiv. 2024 Nov 17:2024.08.24.609500. [Abstract]

Representative fluorescence images of cells upon indicated treatments. AMXT-1501 (0.5 μM; polyamine import inhibitor), DFMO (1 mM), and spermidine (5 μM).
Research Square Preprint

2024 Mar 19.

Lösungsmittel & Löslichkeit

In Vitro:

H₂O : 83.33 mg/mL (116.58 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.3991 mL

6.9953 mL

13.9905 mL

5 mM

0.2798 mL

1.3991 mL

2.7981 mL

10 mM

0.1399 mL

0.6995 mL

1.3991 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 50 mg/mL (69.95 mM); Clear solution; Need ultrasonic

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Zheng J, et al. AMXT-1501 tetrahydrochloride targets membrane phospholipids against Gram-positive and -negative multidrug-resistant bacteria. Emerg Microbes Infect. 2024;13(1):2321981. [Content Brief]

[2]. Ayoola MB, et al. Difluoromethylornithine (DFMO) and AMXT 1501 inhibit capsule biosynthesis in pneumococci. Sci Rep. 2022;12(1):11804. Published 2022 Jul 12. [Content Brief]

[3]. Ayoola MB, Shack LA, Phanstiel O 4th, Nanduri B. Impact of Difluoromethylornithine and AMXT 1501 on Gene Expression and Capsule Regulation in Streptococcus pneumoniae. Biomolecules. 2024 Feb 2;14(2):178. [Content Brief]

[4]. Samal K, Zhao P, Kendzicky A, Yco LP, McClung H, Gerner E, Burns M, Bachmann AS, Sholler G. AMXT-1501 tetrahydrochloride, a novel polyamine transport inhibitor, synergizes with DFMO in inhibiting neuroblastoma cell proliferation by targeting both ornithine decarboxylase and polyamine transport. Int J Cancer. 2013 Sep 15;133(6):1323-33. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	1.3991 mL	6.9953 mL	13.9905 mL	34.9763 mL
	5 mM	0.2798 mL	1.3991 mL	2.7981 mL	6.9953 mL
	10 mM	0.1399 mL	0.6995 mL	1.3991 mL	3.4976 mL
	15 mM	0.0933 mL	0.4664 mL	0.9327 mL	2.3318 mL
	20 mM	0.0700 mL	0.3498 mL	0.6995 mL	1.7488 mL
	25 mM	0.0560 mL	0.2798 mL	0.5596 mL	1.3991 mL
	30 mM	0.0466 mL	0.2332 mL	0.4664 mL	1.1659 mL
	40 mM	0.0350 mL	0.1749 mL	0.3498 mL	0.8744 mL
	50 mM	0.0280 mL	0.1399 mL	0.2798 mL	0.6995 mL
	60 mM	0.0233 mL	0.1166 mL	0.2332 mL	0.5829 mL
	80 mM	0.0175 mL	0.0874 mL	0.1749 mL	0.4372 mL
	100 mM	0.0140 mL	0.0700 mL	0.1399 mL	0.3498 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

AMXT-1501 tetrahydrochloride

11 Cited Publications

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AMXT-1501 tetrahydrochloride

Bioactive Compound Library Max

Bioactive Compound Library

Anti-Infection Compound Library

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Anti-Cancer Compound Library

Antibacterial Compound Library

Orally Active Compound Library

Anti-Cancer Metabolism Compound Library

Cell Death Library

Anti-Drug-Resistant Compound Library

MCE Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) AMXT-1501 tetrahydrochloride

Biologische Aktivität

Chemical Information

Publications (11)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

AMXT-1501 tetrahydrochloride

Bioactive Compound Library Max

Bioactive Compound Library

Anti-Infection Compound Library

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Anti-Cancer Compound Library

Antibacterial Compound Library

Orally Active Compound Library

Anti-Cancer Metabolism Compound Library

Cell Death Library

Anti-Drug-Resistant Compound Library

MCE Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) AMXT-1501 tetrahydrochloride

Biologische Aktivität

Chemical Information

Publications (11)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

AMXT-1501 tetrahydrochloride Related Classifications

HY-124617A Related Classifications