AMXT-1501 tetrahydrochloride
Based on 11 publication(s) in Google Scholar
AMXT-1501 tetrahydrochloride is a Bacterial agent and polyamine transport system inhibitor. AMXT-1501 tetrahydrochloride targets membrane phospholipids and exhibits antibacterial activity against a variety of Gram-positive and Gram-negative multidrug-resistant bacteria. AMXT-1501 tetrahydrochloride inhibits capsular biosynthesis in Streptococcus pneumoniae. AMXT-1501 tetrahydrochloride targets ornithine decarboxylase and polyamines to inhibit the proliferation of neuroblastoma cells. AMXT-1501 tetrahydrochloride in combination with DFMO (HY-B0744) induces Apoptosis in neuroblastoma cells. AMXT-1501 tetrahydrochloride is applicable to research related to multidrug-resistant bacterial infections, pneumococcal infections, Streptococcus pneumoniae infections, and neuroblastoma.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 2444815-84-1
- Formula: C32H72Cl4N6O2
- Molecular Weight:714.77
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Bioactive Compound Library Max
HY-L181
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Bioactive Compound Library
HY-L001
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Anti-Infection Compound Library
HY-L002
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Apoptosis Compound Library
HY-L003
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Immunology/Inflammation Compound Library
HY-L007
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Anti-Cancer Compound Library
HY-L025
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Antibacterial Compound Library
HY-L049
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Orally Active Compound Library
HY-L061
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Anti-Cancer Metabolism Compound Library
HY-L083
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Cell Death Library
HY-L162
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Anti-Drug-Resistant Compound Library
HY-L169
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MCE Bioactive Compound Library
HY-L001V
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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High-Throughput Bioactive Compound Library
HY-L205
Publications Citing Use of MedChemExpress (MCE) AMXT-1501 tetrahydrochloride
More- Immunity. 2025 Aug 12;58(8):2019-2034.e11. [Abstract]
- Nat Immunol. 2026 Jun;27(6):1268-1281. [Abstract]
- Nat Commun. 2024 Mar 19;15(1):2461. [Abstract]
- Cell Death Differ. 2024 Jul;31(7):910-923. [Abstract]
- Neuro Oncol. 2025 Oct 1;27(10):2574-2591. [Abstract]
- Emerg Microbes Infect. 2024 Dec;13(1):2321981. [Abstract]
- Icahn School of Medicine at Mount Sinai. 2025.
- The University of Texas at San Antonio. 2025.
- bioRxiv. 2025 January 26.
- bioRxiv. 2024 Nov 17:2024.08.24.609500. [Abstract]
- Research Square Preprint. 2024 Mar 19.
Customer Validation & Images
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Cell Proliferation/Viability Assay
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WB
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2D/3D Cell Culture and Differentiation
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Cell Imaging/Staining
Biological Activity
Polyamine transport[1]
AMXT-1501 (1.56-50 μM) tetrahydrochloride exhibits dose-dependent antibacterial activity against a variety of Gram-positive and Gram-negative multidrug-resistant (MDR) bacterial isolates, with MIC50/MIC90 values ranging from 3.13 to 12.5 μM[1].
AMXT-1501 (8× MIC; 0-24 h) tetrahydrochloride exhibits rapid and potent bactericidal activity against planktonic MRSA, ESBL-producing E. coli, and CR E. coli[1].
AMXT-1501 (1-4× MIC; 0.5-24 h) tetrahydrochloride reduces biofilm formation in a dose-dependent manner in most tested Staphylococcus aureus (MSSA and MRSA) and Enterococcus faecalis strains, causes severe damage to the cell membranes of MRSA and CR E. coli, increases membrane permeability and depolarizes them, thereby leading to bacterial cell death[1].
AMXT-1501 (3.125-50 μg/mL; 90 s) tetrahydrochloride binds directly to the bacterial membrane phospholipids CL and PG[1].
AMXT-1501 (7.4 μM; cultured until OD600 reaches 0.2-0.5) tetrahydrochloride inhibits capsular polysaccharide biosynthesis by 61% in Streptococcus pneumoniae serotype 2 (strain D39), depletes intracellular polyamines and glucuronic acid, and simultaneously increases intracellular glucose levels[2].
AMXT-1501 (7.4 μM) tetrahydrochloride regulates gene expression in Streptococcus pneumoniae D39, thereby enhancing the biosynthesis of polyamines and glucose, inhibiting ATP production and fatty acid synthesis, and upregulating stress response pathways[3].
AMXT-1501 (0.39-50 μM; 48 h) tetrahydrochloride potently inhibits the viability of human neuroblastoma cell lines BE (2)-C, SMS-KCNR and SH-SY5Y in vitro, with IC50 values of 17.69 μM, 17.72 μM and 14.13 μM, respectively[4].
AMXT-1501 (2.5 μM; 96 h) tetrahydrochloride induced apoptosis in BE (2)-C, SMS-KCNR and SH-SY5Y human neuroblastoma cells when used in combination with DFMO[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:BE(2)-C, SMS-KCNR, SH-SY5Y
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Concentration:0.39 µM, 1 µM, 3.1 µM, 10 µM, 31 µM, 50 µM
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Incubation Time:48 h
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Result:AMXT-1501 tetrahydrochloride exhibited cytotoxicity against this panel of NB cell lines.
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Cell Line:BE(2)‐C, SMS‐KCNR and SH‐SY5Y cells
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Concentration:2.5 μM+2.5 mM DFMO
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Incubation Time:96 h
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Result:Induced apoptosis.
Reduced the amount of uncleaved PARP and increased the amounts of cleaved PARP and cleaved caspase-3.
AMXT-1501 (20 mg/kg; i.p.; every 12 h; 3 days) tetrahydrochloride significantly improves the survival rate of BALB/c mice with bacterial abdominal infection and reduces the bacterial load of CRE E. coli in their lung and liver tissues[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c (female, 6-8-week-old, 18-22 g) (S. aureus YUSA145 (MRSA)(1×107 CFU))[1]
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Dosage:20 mg/kg
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Administration:i.p.; every 12 h; 3 days
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Result:Reduced skin abscess areas significantly.
Lowered bacterial loads in abscess tissue (log10 CFU/g) significantly.
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Animal Model:BALB/c (female, 6-8-week-old, 18-22 g) (S. aureus YUSA145 (MRSA)(1×107 CFU))[1]
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Dosage:20 mg/kg
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Administration:i.p.; every 12 h; 3 days
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Result:Achieved a 50% survival rate through day 7, which was higher than the survival rate in tigecycline-treated mice.
Lowered bacterial loads in lung tissue (log10 CFU/g) significantly.
Lowered bacterial loads in liver tissue (log10 CFU/g) significantly.
Chemical Information
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CAS No. 2444815-84-1
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Appearance Solid
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Molecular Weight 714.77
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Formula C32H72Cl4N6O2
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Color Off-white to light yellow
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SMILES
CCCCCCCCCCCCCCCC(NCCCC[C@@H](N)C(NCCCNCCCCNCCCN)=O)=O.Cl.Cl.Cl.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (11)
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Journal Impact Factor
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Most Recent
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Immunity
Polyamines regulate adaptive antitumor immunity by functional specialization of regulatory T cells. [Abstract]2025 Aug 12;58(8):2019-2034.e11. PMID: 40749666 -
Nat Immunol
Epigenetic reprogramming of T cell metabolism restores function and enhances anti-tumor immunity in lung cancer. [Abstract]2026 Jun;27(6):1268-1281. PMID: 42120791 -
Nat Commun
Polyamine-mediated ferroptosis amplification acts as a targetable vulnerability in cancer. [Abstract]2024 Mar 19;15(1):2461. PMID: 38504107 -
Cell Death Differ
Anaplastic Lymphoma Kinase signaling stabilizes SLC3A2 expression via MARCH11 to promote neuroblastoma cell growth. [Abstract]2024 Jul;31(7):910-923. PMID: 38858548
AMXT-1501 tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2024 Jul;31(7):910-923. [Abstract]
IC50s of AMXT-1501 (4, 8, 12, 16, 20, 24 μM) in CLB-BAR, CLB-GE, SK-N-BE(2) and SH-SY5Y cells after 5 d treatment.
AMXT-1501 tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2024 Jul;31(7):910-923. [Abstract]
Immunoblot analysis of neuronal differentiation markers (RET and DLG2) in SK-N-BE(2) and SH-SY5Y cells treated with AMXT-1501 (0 or 8 µM) and RA (0 or 5 µM) for 48 h or 24 h .
AMXT-1501 tetrahydrochloride purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2024 Jul;31(7):910-923. [Abstract]
Cells after AMXT-1501 (0 or 8 µM) and RA (0 or 5 µM) treatment as indicated for 48 h or 24 h.
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Neuro Oncol
Polyamine acetylation mediates crosstalk between cancer cells and myeloid cells to promote mesenchymal/plurimetabolic states in glioblastoma. [Abstract]2025 Oct 1;27(10):2574-2591. PMID: 40424600 -
Emerg Microbes Infect
AMXT-1501 targets membrane phospholipids against Gram-positive and -negative multidrug-resistant bacteria. [Abstract]2024 Dec;13(1):2321981. PMID: 38422452 -
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bioRxiv
2024 Nov 17:2024.08.24.609500. PMID: 39253442
AMXT-1501 tetrahydrochloride purchased from MedChemExpress. Usage Cited in: bioRxiv. 2024 Nov 17:2024.08.24.609500. [Abstract]
Representative fluorescence images of cells upon indicated treatments. AMXT-1501 (0.5 μM; polyamine import inhibitor), DFMO (1 mM), and spermidine (5 μM).
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Solvent & Solubility
H2O : 83.33 mg/mL (116.58 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 50 mg/mL (69.95 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Zheng J, et al. AMXT-1501 tetrahydrochloride targets membrane phospholipids against Gram-positive and -negative multidrug-resistant bacteria. Emerg Microbes Infect. 2024;13(1):2321981. [Content Brief]
[2]. Ayoola MB, et al. Difluoromethylornithine (DFMO) and AMXT 1501 inhibit capsule biosynthesis in pneumococci. Sci Rep. 2022;12(1):11804. Published 2022 Jul 12. [Content Brief]
[3]. Ayoola MB, Shack LA, Phanstiel O 4th, Nanduri B. Impact of Difluoromethylornithine and AMXT 1501 on Gene Expression and Capsule Regulation in Streptococcus pneumoniae. Biomolecules. 2024 Feb 2;14(2):178. [Content Brief]
[4]. Samal K, Zhao P, Kendzicky A, Yco LP, McClung H, Gerner E, Burns M, Bachmann AS, Sholler G. AMXT-1501 tetrahydrochloride, a novel polyamine transport inhibitor, synergizes with DFMO in inhibiting neuroblastoma cell proliferation by targeting both ornithine decarboxylase and polyamine transport. Int J Cancer. 2013 Sep 15;133(6):1323-33. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O | 1 mM | 1.3991 mL | 6.9953 mL | 13.9905 mL | 34.9763 mL |
| 5 mM | 0.2798 mL | 1.3991 mL | 2.7981 mL | 6.9953 mL | |
| 10 mM | 0.1399 mL | 0.6995 mL | 1.3991 mL | 3.4976 mL | |
| 15 mM | 0.0933 mL | 0.4664 mL | 0.9327 mL | 2.3318 mL | |
| 20 mM | 0.0700 mL | 0.3498 mL | 0.6995 mL | 1.7488 mL | |
| 25 mM | 0.0560 mL | 0.2798 mL | 0.5596 mL | 1.3991 mL | |
| 30 mM | 0.0466 mL | 0.2332 mL | 0.4664 mL | 1.1659 mL | |
| 40 mM | 0.0350 mL | 0.1749 mL | 0.3498 mL | 0.8744 mL | |
| 50 mM | 0.0280 mL | 0.1399 mL | 0.2798 mL | 0.6995 mL | |
| 60 mM | 0.0233 mL | 0.1166 mL | 0.2332 mL | 0.5829 mL | |
| 80 mM | 0.0175 mL | 0.0874 mL | 0.1749 mL | 0.4372 mL | |
| 100 mM | 0.0140 mL | 0.0700 mL | 0.1399 mL | 0.3498 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
AMXT-1501 tetrahydrochloride Related Classifications
HY-124617A Related Classifications
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