Eflornithine (Synonyms: DFMO; MDL71782; RMI71782; α-difluoromethylornithine)

Name: Eflornithine
Brand: MedChemExpress
SKU: HY-B0744
Rating: 5.0 (21 reviews)

Cat. No.: HY-B0744 Purity: 99.87%: Data Sheet
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Eflornithine is a specific, irreversible inhibitor of the enzyme ornithine decarboxylase. Eflornithine is a medication for the treatment of African trypanosomiasis and excessive facial hair growth in women.

For research use only. We do not sell to patients.

CAS No. : 70052-12-9

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	Get quote
5 g	Get quote

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Based on 21 publication(s) in Google Scholar

Other Forms of Eflornithine:

L-Eflornithine monohydrochloride In-stock
Eflornithine hydrochloride In-stock
Eflornithine hydrochloride hydrate In-stock
L-Eflornithine Get quote

21 Publications Citing Use of MCE Eflornithine

Flow Cytometry

Histological Imaging/Staining

In Vivo Efficacy Study

RT-PCR

Bio/Physico-chemical Assay

: Eflornithine purchased from MedChemExpress. Usage Cited in: Immunity. 2025 Aug 12;58(8):2019-2034.e11. [Abstract]; Csnk2b^Treg−/− and Csnk2b^fl/fl mice were inoculated with B16.F10 cells and then orally administered Eflornithine hydrochloride hydrate (1% w/v) in combination with subcutaneous injection of AMXT 1501 around the tumor. We measured tumor growth and analyzed the phenotype of Treg cells in the tumor microenvironment (TME) using multicolor flow cytometry. The combination therapy had no effect on tumor growth in Csnk2b^Treg−/− mice but significantly inhibited tumor growth in Csnk2b^fl/fl mice.

: Eflornithine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 21:e12508. [Abstract]; Bar graphs showing the proportion of ALDH+ cells in OVCAR3, OVCAR8, and SKOV3 treated with THP-1-derived Mφ+ACs CM, THP-1-derived Mφ+ACs treated with 1 mM Eflornithine CM, respectively, for 48 h were presented.

: Eflornithine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 21:e12508. [Abstract]; Bar graph showing the relative mRNA expression of SOX2, ALDH1A1, and ALDH1A3 in OVCAR3 treated with THP-1 derived Mφ +ACs CM, THP-1 derived Mφ+ACs treated with 1 mM Eflornithine CM separately for 48 h.

: Eflornithine purchased from MedChemExpress. Usage Cited in: Sci Adv. 2023 May 19;9(20):eade0718. [Abstract]; Percentage of IFN-γ+ and mean fluorescence intensity (MFI) of Ki-67 in CD8⁺ T cells after anti-CD3 antibody stimulation versus anti-CD3 antibody plus rSema4A stimulation with or without Sardomozide Dihydrochloride (10 μM) and Eflornithine hydrochloride hydrate (500 μM).

: Eflornithine purchased from MedChemExpress. Usage Cited in: Commun Biol. 2019 May 8:2:171. [Abstract]; Eflornithine (DFMO) was administered as a 1% solution in drinking distilled H₂O to mice for 14 days. The mean DFMO consumption of mice was ∼1.5 g/kg/d. Immunostaining of IDO1 in the ileum after L-Orn administration in wt mice or after L-Orn inhibitor DFMO administration in human REG3A^tg mice was performed.

: Eflornithine purchased from MedChemExpress. Usage Cited in: Commun Biol. 2019 May 8:2:171. [Abstract]; Eflornithine (DFMO) was administered as a 1% solution in drinking distilled H₂O to mice for 14 days. The mean DFMO consumption of mice was ∼1.5 g/kg/d. HPLC/MASS of L-Kyn in the ileum epithelial cells after administration of L-Orn or L-Orn inhibitor DFMO.

: Eflornithine purchased from MedChemExpress. Usage Cited in: Commun Biol. 2019 May 8:2:171. [Abstract]; Eflornithine (DFMO) was administered as a 1% solution in drinking distilled H₂O to mice for 14 days. The mean DFMO consumption of mice was ∼1.5 g/kg/d. Flow cytometry of RORγt (+) IL-22(+) cells in the ileum of mice after administering L-Orn or L-Orn inhibitor DFMO.

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Trypanosoma

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	> 100 μM Compound: Eflornithine	Cytotoxicity against HEK293 cells assessed as reduction in cell viability after 48 hrs by alamar blue assay Cytotoxicity against HEK293 cells assessed as reduction in cell viability after 48 hrs by alamar blue assay	[PMID: 27720295]
HepG2	CC₅₀	> 100 μM Compound: Eflornithine	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 32292551]
HepG2	CC₅₀	> 100 μM Compound: Eflornithine	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 32652409]
HepG2	CC₅₀	> 100 μM Compound: Eflornithine	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 32795774]
L6	IC₅₀	12 μg/mL Compound: Eflornithine	Cytotoxicity against in rat L6 cells assessed as reduction in cell viability after 70 hrs by alamar blue assay Cytotoxicity against in rat L6 cells assessed as reduction in cell viability after 70 hrs by alamar blue assay	[PMID: 21353728]
MRC5	IC₅₀	> 100 μg/mL Compound: Eflornithine	Cytotoxicity against human MRC5 cells Cytotoxicity against human MRC5 cells	[PMID: 24388808]
MRC5	IC₅₀	> 100 μg/mL Compound: eflornithine	Cytotoxicity against human MRC5 cells after 7 days by MTT assay Cytotoxicity against human MRC5 cells after 7 days by MTT assay	[PMID: 19013823]

In Vivo

Eflornithine is the only new molecule registered for the treatment of human African trypanosomiasis over the last 50 years. It is the drug used mainly as a back-up for melarsoprol refractory Trypanosoma brucei gambiense cases^[1]. In subjects with excessive, unwanted facial hair, eflornithine 15% cream is superior to placebo in reducing hair growth. After 24 weeks' treatment, 58% of eflornithine and 34% of placebo subjects have at least some improvement in facial hirsutism^[2]. The hair growth inhibitory activity of eflornithine is significantly enhanced when the eflornithine cream is applied onto a mouse skin area pretreated with microneedles^[3]. Treatment of coarctation hypertensive rats with eflornithine results in a normalization of the contractile intensity to KCI and norepinephrine and relaxations to acetylcholine by 14 days of hypertension^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

182.17

Formula

C₆H₁₂F₂N₂O₂

CAS No.

70052-12-9

Appearance

Solid

Color

White to light yellow

SMILES

NC(CCCN)(C(F)F)C(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 83.33 mg/mL (457.43 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.4894 mL	27.4469 mL	54.8938 mL
5 mM	1.0979 mL	5.4894 mL	10.9788 mL
10 mM	0.5489 mL	2.7447 mL	5.4894 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

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Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.87%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (245 KB) HNMR (131 KB) RP-HPLC (237 KB) MS (71 KB)

Handling Instructions (2659 KB)

References

[1]. Burri C, et al. Eflornithine for the treatment of human African trypanosomiasis. Parasitol Res. 2003 Jun;90 Supp 1:S49-52. [Content Brief]

[2]. Balfour JA, et al. Topical eflornithine. Am J Clin Dermatol. 2001;2(3):197-201; discussion 202. [Content Brief]

[3]. Kumar A, et al. A method to improve the efficacy of topical eflornithine hydrochloride cream. Drug Deliv. 2016 Jun;23(5):1495-501. [Content Brief]

[4]. Lipke DW, et al. Eflornithine alters changes in vascular responsiveness associated with coarctation hypertension. Clin Exp Hypertens. 1997 Apr;19(3):297-312. [Content Brief]

Animal Administration
^[3]

Mice: The skin area where the hair is removed is then treated with the eflornithine hydrochloride 13.9% cream (∼50 mg per mouse per treatment) using a spatula 2 times a day in an interval of at least 8 h for a maximum period of 36 days^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Burri C, et al. Eflornithine for the treatment of human African trypanosomiasis. Parasitol Res. 2003 Jun;90 Supp 1:S49-52. [Content Brief]

[2]. Balfour JA, et al. Topical eflornithine. Am J Clin Dermatol. 2001;2(3):197-201; discussion 202. [Content Brief]

[3]. Kumar A, et al. A method to improve the efficacy of topical eflornithine hydrochloride cream. Drug Deliv. 2016 Jun;23(5):1495-501. [Content Brief]

[4]. Lipke DW, et al. Eflornithine alters changes in vascular responsiveness associated with coarctation hypertension. Clin Exp Hypertens. 1997 Apr;19(3):297-312. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	5.4894 mL	27.4469 mL	54.8938 mL	137.2345 mL
	5 mM	1.0979 mL	5.4894 mL	10.9788 mL	27.4469 mL
	10 mM	0.5489 mL	2.7447 mL	5.4894 mL	13.7234 mL
	15 mM	0.3660 mL	1.8298 mL	3.6596 mL	9.1490 mL
	20 mM	0.2745 mL	1.3723 mL	2.7447 mL	6.8617 mL
	25 mM	0.2196 mL	1.0979 mL	2.1958 mL	5.4894 mL
	30 mM	0.1830 mL	0.9149 mL	1.8298 mL	4.5745 mL
	40 mM	0.1372 mL	0.6862 mL	1.3723 mL	3.4309 mL
	50 mM	0.1098 mL	0.5489 mL	1.0979 mL	2.7447 mL
	60 mM	0.0915 mL	0.4574 mL	0.9149 mL	2.2872 mL
	80 mM	0.0686 mL	0.3431 mL	0.6862 mL	1.7154 mL
	100 mM	0.0549 mL	0.2745 mL	0.5489 mL	1.3723 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Eflornithine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Eflornithine (Synonyms: DFMO; MDL71782; RMI71782; α-difluoromethylornithine)

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Other Forms of Eflornithine:

Eflornithine Related Antibodies

21 Publications Citing Use of MCE Eflornithine

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View All Parasite Isoform Specific Products:

Biological Activity

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References

Customer Review

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Complete Stock Solution Preparation Table

Eflornithine Related Classifications

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