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  4. Eflornithine hydrochloride hydrate

Eflornithine hydrochloride hydrate  (Synonyms: DFMO hydrochloride hydrate; MDL-71782 hydrochloride hydrate; RMI-71782 hydrochloride hydrate; α-difluoromethylornithine hydrochloride hydrate)

Cat. No.: HY-B0744B Purity: 99.90%
COA
SDS
Handling Instructions Technical Support

Eflornithine hydrochloride hydrate (DFMO hydrochloride hydrate) is a specific, irreversible inhibitor of the enzyme ornithine decarboxylase. Eflornithine hydrochloride hydrate is a medication for the treatment of African trypanosomiasis and excessive facial hair growth in women.

For research use only. We do not sell to patients.

CAS No. : 96020-91-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in Water In-stock
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500 mg In-stock
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Customer Review

Based on 21 publication(s) in Google Scholar

Other Forms of Eflornithine hydrochloride hydrate:

Eflornithine hydrochloride hydrate Related Antibodies

Top Publications Citing Use of Products

    Eflornithine hydrochloride hydrate purchased from MedChemExpress. Usage Cited in: Immunity. 2025 Aug 12;58(8):2019-2034.e11.  [Abstract]

    Csnk2bTreg−/− and Csnk2bfl/fl mice were inoculated with B16.F10 cells and then orally administered Eflornithine hydrochloride hydrate (1% w/v) in combination with subcutaneous injection of AMXT 1501 around the tumor. We measured tumor growth and analyzed the phenotype of Treg cells in the tumor microenvironment (TME) using multicolor flow cytometry. The combination therapy had no effect on tumor growth in Csnk2bTreg−/− mice but significantly inhibited tumor growth in Csnk2bfl/fl mice.

    Eflornithine hydrochloride hydrate purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 21:e12508.  [Abstract]

    Bar graphs showing the proportion of ALDH+ cells in OVCAR3, OVCAR8, and SKOV3 treated with THP-1-derived Mφ+ACs CM, THP-1-derived Mφ+ACs treated with 1 mM Eflornithine CM, respectively, for 48 h were presented.

    Eflornithine hydrochloride hydrate purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 21:e12508.  [Abstract]

    Bar graph showing the relative mRNA expression of SOX2, ALDH1A1, and ALDH1A3 in OVCAR3 treated with THP-1 derived Mφ +ACs CM, THP-1 derived Mφ+ACs treated with 1 mM Eflornithine CM separately for 48 h.

    Eflornithine hydrochloride hydrate purchased from MedChemExpress. Usage Cited in: Sci Adv. 2023 May 19;9(20):eade0718.  [Abstract]

    Percentage of IFN-γ+ and mean fluorescence intensity (MFI) of Ki-67 in CD8+ T cells after anti-CD3 antibody stimulation versus anti-CD3 antibody plus rSema4A stimulation with or without Sardomozide Dihydrochloride (10 μM) and Eflornithine hydrochloride hydrate (500 μM).

    Eflornithine hydrochloride hydrate purchased from MedChemExpress. Usage Cited in: Commun Biol. 2019 May 8:2:171.  [Abstract]

    Eflornithine (DFMO) was administered as a 1% solution in drinking distilled H₂O to mice for 14 days. The mean DFMO consumption of mice was ∼1.5 g/kg/d. Immunostaining of IDO1 in the ileum after L-Orn administration in wt mice or after L-Orn inhibitor DFMO administration in human REG3Atg mice was performed.

    Eflornithine hydrochloride hydrate purchased from MedChemExpress. Usage Cited in: Commun Biol. 2019 May 8:2:171.  [Abstract]

    Eflornithine (DFMO) was administered as a 1% solution in drinking distilled H₂O to mice for 14 days. The mean DFMO consumption of mice was ∼1.5 g/kg/d. HPLC/MASS of L-Kyn in the ileum epithelial cells after administration of L-Orn or L-Orn inhibitor DFMO.

    Eflornithine hydrochloride hydrate purchased from MedChemExpress. Usage Cited in: Commun Biol. 2019 May 8:2:171.  [Abstract]

    Eflornithine (DFMO) was administered as a 1% solution in drinking distilled H₂O to mice for 14 days. The mean DFMO consumption of mice was ∼1.5 g/kg/d. Flow cytometry of RORγt (+) IL-22(+) cells in the ileum of mice after administering L-Orn or L-Orn inhibitor DFMO.

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    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Eflornithine hydrochloride hydrate (DFMO hydrochloride hydrate) is a specific, irreversible inhibitor of the enzyme ornithine decarboxylase. Eflornithine hydrochloride hydrate is a medication for the treatment of African trypanosomiasis and excessive facial hair growth in women[1].

    IC50 & Target

    Trypanosoma

     

    Cellular Effect
    Cell Line Type Value Description References
    J774 IC50
    > 1000 μM
    Compound: S3
    Cytotoxicity against J774.1 cell line after 48 hrs
    Cytotoxicity against J774.1 cell line after 48 hrs
    		[PMID: 16516467]
    In Vivo

    The hair growth inhibitory activity of Eflornithine is significantly enhanced when the Eflornithine cream is applied onto a mouse skin area pretreated with microneedles[3]. Treatment of coarctation hypertensive rats with Eflornithine results in a normalization of the contractile intensity to KCI and norepinephrine and relaxations to acetylcholine by 14 days of hypertension[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    236.64

    Formula

    C6H15ClF2N2O3
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    NC(CCCN)(C(F)F)C(O)=O.[H]Cl.O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 83.33 mg/mL (352.14 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.2258 mL 21.1291 mL 42.2583 mL
    5 mM 0.8452 mL 4.2258 mL 8.4517 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (422.58 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.90%

    SDS (396 KB)

    COA (249 KB) HNMR (235 KB) RP-HPLC (238 KB) MS (70 KB)

    Handling Instructions (2659 KB)
    References
    Animal Administration
    [3]

    Mice: The skin area where the hair is removed is then treated with the eflornithine hydrochloride 13.9% cream (-50 mg per mouse per treatment) using a spatula 2 times a day in an interval of at least 8 h for a maximum period of 36 days[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 4.2258 mL 21.1291 mL 42.2583 mL 105.6457 mL
    5 mM 0.8452 mL 4.2258 mL 8.4517 mL 21.1291 mL
    10 mM 0.4226 mL 2.1129 mL 4.2258 mL 10.5646 mL
    15 mM 0.2817 mL 1.4086 mL 2.8172 mL 7.0430 mL
    20 mM 0.2113 mL 1.0565 mL 2.1129 mL 5.2823 mL
    25 mM 0.1690 mL 0.8452 mL 1.6903 mL 4.2258 mL
    30 mM 0.1409 mL 0.7043 mL 1.4086 mL 3.5215 mL
    40 mM 0.1056 mL 0.5282 mL 1.0565 mL 2.6411 mL
    50 mM 0.0845 mL 0.4226 mL 0.8452 mL 2.1129 mL
    60 mM 0.0704 mL 0.3522 mL 0.7043 mL 1.7608 mL
    80 mM 0.0528 mL 0.2641 mL 0.5282 mL 1.3206 mL
    100 mM 0.0423 mL 0.2113 mL 0.4226 mL 1.0565 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Eflornithine hydrochloride hydrate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Eflornithine hydrochloride96020-91-6DFMO hydrochlorideMDL-71782 hydrochlorideRMI-71782 hydrochlorideα-difluoromethylornithine hydrochlorideParasiteInhibitorinhibitorinhibit

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