Eflornithine hydrochloride
Based on 23 publication(s) in Google Scholar
Eflornithine hydrochloride is a specific, irreversible inhibitor of the enzyme ornithine decarboxylase. Eflornithine is a medication for the treatment of African trypanosomiasis and excessive facial hair growth in women.
For research use only. We do not sell to patients.
- Purity: 99.90%
- CAS No.: 68278-23-9
- Formula: C6H13ClF2N2O2
- Molecular Weight:218.63
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Publications Citing Use of MedChemExpress (MCE) Eflornithine hydrochloride
More- Nature. 2026 Jul;655(8121):240-250. [Abstract]
- Immunity. 2025 Aug 12;58(8):2019-2034.e11. [Abstract]
- Nat Commun. 2025 Dec 8. [Abstract]
- Nat Commun. 2024 Mar 19;15(1):2461. [Abstract]
- J Clin Invest. 2026 Jun 11.
- Adv Sci (Weinh). 2025 Nov 21:e12508. [Abstract]
- Sci Adv. 2023 May 19;9(20):eade0718. [Abstract]
- Phytomedicine. 2025 Jun:141:156660. [Abstract]
- Clin Cancer Res. 2025 Nov 20. [Abstract]
- JACC Basic Transl Sci. 2022 Aug 3;7(8):820-840. [Abstract]
- Cell Rep. 2025 Feb 28;44(3):115370. [Abstract]
- Cell Rep. 2025 Jan 28;44(1):115123. [Abstract]
- Food Biosci. 2023 Oct, 55, 102970.
- RSC Adv. 2019 Apr 9;9(20):11026-11037. [Abstract]
- Cancer Nanotechnol. 2023 May 9.
- Commun Biol. 2019 May 8:2:171. [Abstract]
- Front Oncol. 2021 Mar 11:11:636373. [Abstract]
- J Cardiovasc Transl Res. 2025 Jun;18(3):459-470. [Abstract]
- Biochem Biophys Res Commun. 2026 Feb 12:800:153251. [Abstract]
- Onco Targets Ther. 2020 Nov 17:13:11697-11709. [Abstract]
- The University of Texas at San Antonio. 2025.
- Preprints. 2025 May 22.
- bioRxiv. 2024 Jan 15.
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In Vivo Efficacy Study
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Flow Cytometry
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Biological Activity
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Trypanosoma |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| J774 | IC50 |
>1000 μM
Compound: S3
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Cytotoxicity against J774.1 cell line after 48 hrs
Cytotoxicity against J774.1 cell line after 48 hrs
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[PMID: 16516467] |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 68278-23-9
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Appearance Solid
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Molecular Weight 218.63
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Formula C6H13ClF2N2O2
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Color White to light yellow
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SMILES
NC(CCCN)(C(F)F)C(O)=O.[H]Cl
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Synonyms
DFMO hydrochloride; MDL71782 hydrochloride; RMI71782 hydrochloride; α-difluoromethylornithine hydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (23)
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Journal Impact Factor
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Most Recent
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Nature
2026 Jul;655(8121):240-250. PMID: 42236947 -
Immunity
Polyamines regulate adaptive antitumor immunity by functional specialization of regulatory T cells. [Abstract]2025 Aug 12;58(8):2019-2034.e11. PMID: 40749666
Eflornithine hydrochloride purchased from MedChemExpress. Usage Cited in: Immunity. 2025 Aug 12;58(8):2019-2034.e11. [Abstract]
Csnk2bTreg−/− and Csnk2bfl/fl mice were inoculated with B16.F10 cells and then orally administered Eflornithine hydrochloride hydrate (1% w/v) in combination with subcutaneous injection of AMXT 1501 around the tumor. We measured tumor growth and analyzed the phenotype of Treg cells in the tumor microenvironment (TME) using multicolor flow cytometry. The combination therapy had no effect on tumor growth in Csnk2bTreg−/− mice but significantly inhibited tumor growth in Csnk2bfl/fl mice.
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Nat Commun
2025 Dec 8. PMID: 41361241 -
Nat Commun
Polyamine-mediated ferroptosis amplification acts as a targetable vulnerability in cancer. [Abstract]2024 Mar 19;15(1):2461. PMID: 38504107 -
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Adv Sci (Weinh)
Efferocytosis-Driven Polyamine Metabolism in Macrophages Enhances Cancer Stem Cell Enrichment after Chemotherapy in Ovarian Cancer. [Abstract]2025 Nov 21:e12508. PMID: 41271556
Eflornithine hydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 21:e12508. [Abstract]
Bar graphs showing the proportion of ALDH+ cells in OVCAR3, OVCAR8, and SKOV3 treated with THP-1-derived Mφ+ACs CM, THP-1-derived Mφ+ACs treated with 1 mM Eflornithine CM, respectively, for 48 h were presented.
Eflornithine hydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 21:e12508. [Abstract]
Bar graph showing the relative mRNA expression of SOX2, ALDH1A1, and ALDH1A3 in OVCAR3 treated with THP-1 derived Mφ +ACs CM, THP-1 derived Mφ+ACs treated with 1 mM Eflornithine CM separately for 48 h.
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Sci Adv
Tumor-derived semaphorin 4A improves PD-1-blocking antibody efficacy by enhancing CD8+ T cell cytotoxicity and proliferation. [Abstract]2023 May 19;9(20):eade0718. PMID: 37205755
Eflornithine hydrochloride purchased from MedChemExpress. Usage Cited in: Sci Adv. 2023 May 19;9(20):eade0718. [Abstract]
Percentage of IFN-γ+ and mean fluorescence intensity (MFI) of Ki-67 in CD8+ T cells after anti-CD3 antibody stimulation versus anti-CD3 antibody plus rSema4A stimulation with or without Sardomozide Dihydrochloride (10 μM) and Eflornithine hydrochloride hydrate (500 μM).
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Phytomedicine
Oleic acid regulates CD4+ T cells differentiation by targeting ODC1-mediated STAT5A phosphorylation in Vogt-Koyanagi-Harada disease. [Abstract]2025 Jun:141:156660. PMID: 40203473 -
Clin Cancer Res
Spermidine Secreted by Apoptotic Cells Enhances Chemotherapy Resistance by Modulating β-Catenin Activity in Osteosarcoma. [Abstract]2025 Nov 20. PMID: 41263700 -
JACC Basic Transl Sci
Targeting Myocardial Mitochondria-STING-Polyamine Axis Prevents Cardiac Hypertrophy in Chronic Kidney Disease. [Abstract]2022 Aug 3;7(8):820-840. PMID: 36061341 -
Cell Rep
Intestinal microbiota affects the progression of colorectal cancer by participating in the host intestinal arginine catabolism. [Abstract]2025 Feb 28;44(3):115370. PMID: 40022728 -
Cell Rep
2025 Jan 28;44(1):115123. PMID: 39932195 -
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RSC Adv
Spermine modified polymeric micelles with pH-sensitive drug release for targeted and enhanced antitumor therapy. [Abstract]2019 Apr 9;9(20):11026-11037. PMID: 35520220 -
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Commun Biol
2019 May 8:2:171. PMID: 31098404
Eflornithine hydrochloride purchased from MedChemExpress. Usage Cited in: Commun Biol. 2019 May 8:2:171. [Abstract]
Eflornithine (DFMO) was administered as a 1% solution in drinking distilled H₂O to mice for 14 days. The mean DFMO consumption of mice was ∼1.5 g/kg/d. Immunostaining of IDO1 in the ileum after L-Orn administration in wt mice or after L-Orn inhibitor DFMO administration in human REG3Atg mice was performed.
Eflornithine hydrochloride purchased from MedChemExpress. Usage Cited in: Commun Biol. 2019 May 8:2:171. [Abstract]
Eflornithine (DFMO) was administered as a 1% solution in drinking distilled H₂O to mice for 14 days. The mean DFMO consumption of mice was ∼1.5 g/kg/d. HPLC/MASS of L-Kyn in the ileum epithelial cells after administration of L-Orn or L-Orn inhibitor DFMO.
Eflornithine hydrochloride purchased from MedChemExpress. Usage Cited in: Commun Biol. 2019 May 8:2:171. [Abstract]
Eflornithine (DFMO) was administered as a 1% solution in drinking distilled H₂O to mice for 14 days. The mean DFMO consumption of mice was ∼1.5 g/kg/d. Flow cytometry of RORγt (+) IL-22(+) cells in the ileum of mice after administering L-Orn or L-Orn inhibitor DFMO.
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Front Oncol
Dual Inhibition of Ornithine Decarboxylase and A1 Adenosine Receptor Efficiently Suppresses Breast Tumor Cells. [Abstract]2021 Mar 11:11:636373. PMID: 33777791 -
J Cardiovasc Transl Res
LACC1 Enhances Polyamine Immunometabolism in Inflammatory Macrophages to Inhibit Atherosclerosis Progression. [Abstract]2025 Jun;18(3):459-470. PMID: 40293654 -
Biochem Biophys Res Commun
Alpha-difluoromethylornithine suppresses angiogenesis via the FLI1-CLEC14A-VEGFC pathway in retinal endothelial cells. [Abstract]2026 Feb 12:800:153251. PMID: 41548484 -
Onco Targets Ther
Simultaneous Inhibition of Ornithine Decarboxylase 1 and Pyruvate Kinase M2 Exerts Synergistic Effects Against Hepatocellular Carcinoma Cells. [Abstract]2020 Nov 17:13:11697-11709. PMID: 33244237 -
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Solvent & Solubility
H2O : 100 mg/mL (457.39 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
Mice: The skin area where the hair is removed is then treated with the eflornithine hydrochloride 13.9% cream (∼50 mg per mouse per treatment) using a spatula 2 times a day in an interval of at least 8 h for a maximum period of 36 days[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Burri C, et al. Eflornithine for the treatment of human African trypanosomiasis. Parasitol Res. 2003 Jun;90 Supp 1:S49-52. [Content Brief]
[2]. Balfour JA, et al. Topical eflornithine. Am J Clin Dermatol. 2001;2(3):197-201; discussion 202. [Content Brief]
[3]. Kumar A, et al. A method to improve the efficacy of topical eflornithine hydrochloride cream. Drug Deliv. 2016 Jun;23(5):1495-501. [Content Brief]
[4]. Lipke DW, et al. Eflornithine alters changes in vascular responsiveness associated with coarctation hypertension. Clin Exp Hypertens. 1997 Apr;19(3):297-312. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 4.5739 mL | 22.8697 mL | 45.7394 mL | 114.3484 mL |
| 5 mM | 0.9148 mL | 4.5739 mL | 9.1479 mL | 22.8697 mL | |
| 10 mM | 0.4574 mL | 2.2870 mL | 4.5739 mL | 11.4348 mL | |
| 15 mM | 0.3049 mL | 1.5246 mL | 3.0493 mL | 7.6232 mL | |
| 20 mM | 0.2287 mL | 1.1435 mL | 2.2870 mL | 5.7174 mL | |
| 25 mM | 0.1830 mL | 0.9148 mL | 1.8296 mL | 4.5739 mL | |
| 30 mM | 0.1525 mL | 0.7623 mL | 1.5246 mL | 3.8116 mL | |
| 40 mM | 0.1143 mL | 0.5717 mL | 1.1435 mL | 2.8587 mL | |
| 50 mM | 0.0915 mL | 0.4574 mL | 0.9148 mL | 2.2870 mL | |
| 60 mM | 0.0762 mL | 0.3812 mL | 0.7623 mL | 1.9058 mL | |
| 80 mM | 0.0572 mL | 0.2859 mL | 0.5717 mL | 1.4294 mL | |
| 100 mM | 0.0457 mL | 0.2287 mL | 0.4574 mL | 1.1435 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Eflornithine hydrochloride Related Classifications
HY-B0744A Related Classifications
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