Bradykinin
Based on 18 publication(s) in Google Scholar
Bradykinin is an effective endothelium-dependent vasodilator that can lower blood pressure. Bradykinin can induce contraction of bronchial and intestinal non-vascular smooth muscle, increase vascular permeability, and participate in the mechanism of pain.
For research use only. We do not sell to patients.
- Purity: 99.98%
- CAS No.: 58-82-2
- Formula: C50H73N15O11
- Molecular Weight:1060.21
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Bradykinin
More- Chem Eng J. 2026 Jan 27;530:173437.
- Anal Chem. 2025 Nov 4;97(43):24225-24231. [Abstract]
- Anal Chem. 2024 Aug 17. [Abstract]
- Anal Chem. 2022 Apr 26;94(16):6363-6370. [Abstract]
- Talanta. 2024 Oct 14:282:127045. [Abstract]
- iScience. 2024 May 22;27(6):110075. [Abstract]
- iScience. 2022 Nov 8;25(11):105446. [Abstract]
- ACS Chem Neurosci. 2021 Jul 21;12(14):2619-2628. [Abstract]
- J Am Soc Mass Spectrom. 2020 Jul 5. [Abstract]
- SLAS Discov. 2019 Feb;24(2):158-168. [Abstract]
- World J Surg Oncol. 2019 Feb 27;17(1):44. [Abstract]
- Biochem Biophys Res Commun. 2024 Dec 31:746:151265. [Abstract]
- Rapid Commun Mass Spectrom. 2023 Feb 15;37(3):e9429. [Abstract]
- Int J Mass Spectrom. 14 October 2021, 116727.
- Int Heart J. 2025 May 31;66(3):485-496. [Abstract]
- bioRxiv. 2026 Mar 30.
- bioRxiv. 2025 Sep 28.
- bioRxiv. 2023 Sep 1.
Customer Validation & Images
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WB
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Flow Cytometry
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Cell Imaging/Staining
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Cell Imaging/Staining
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Histological Imaging/Staining
All Bradykinin Receptor Isoforms
MoreAll Endogenous Metabolite Isoforms
More
Biological Activity
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Bradykinin B2 Receptor (B2R) |
Human Endogenous Metabolite |
Bradykinin is a potent vasodilator peptide that exerts its vasodilatory action through stimulation of specific endothelial B2 receptors, thereby causing the release of prostacyclin, NO, and EDHF[1]. Bradykinin has been reported to be involved in the progression of many types of cancer. Bradykinin treatment promotes the invasion and migration of colorectal cancer cells. Bradykinin treatment stimulates ERK1/2 activation and IL-6 production[2]. Exogenous bradykinin markedly inhibits TF expression in mRNA and protein level induced by LPS in a dose-dependent manner. The NO synthase antagonist L-NAME and PI3K inhibitor LY294002 dramatically abolish the inhibitory effects of bradykinin on tissue factor expression[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 58-82-2
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Appearance Solid
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Molecular Weight 1060.21
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Formula C50H73N15O11
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Color White to off-white
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Sequence
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
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Sequence Shortening
RPPGFSPFR
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (18)
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Journal Impact Factor
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Most Recent
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Anal Chem
Two-Dimensional FAIMS-TIMS Separation for Probing Structural Diversity of Peptides with Resolution Exceeding 6500. [Abstract]2025 Nov 4;97(43):24225-24231. PMID: 41116333 -
Anal Chem
Racetrack FAIMS for High-Resolution and High-Sensitivity Characterization of Peptide Conformers. [Abstract]2024 Aug 17. PMID: 39153009 -
Anal Chem
Two-Dimensional FAIMS-IMS Characterization of Peptide Conformers with Resolution Exceeding 1000. [Abstract]2022 Apr 26;94(16):6363-6370. PMID: 35412805 -
Talanta
A robust polymetallic-coated sheathless interface with high acid and alkali resistance for coupling capillary electrophoresis with mass spectrometry. [Abstract]2024 Oct 14:282:127045. PMID: 39418980 -
iScience
Bradykinin induces acute kidney injury after hypothermic circulatory arrest through the repression of the Nrf2-xCT pathway. [Abstract]2024 May 22;27(6):110075. PMID: 38868208
Bradykinin purchased from MedChemExpress. Usage Cited in: iScience. 2024 May 22;27(6):110075. [Abstract]
Representative Western blot images of Nrf2, xCT and GPX4 expression in HK-2 cells treated with 100 μM H2O2 and different concentrations of Bradykinin (0, 10, 50, 100, 500, 1000 nM) for 24 h.
Bradykinin purchased from MedChemExpress. Usage Cited in: iScience. 2024 May 22;27(6):110075. [Abstract]
Flow cytometry of reactive oxygen species (ROS) in HK-2 cells treated with 100 μM H2O2 alone or 100 μM H2O2 plus 100 nM Bradykinin.
Bradykinin purchased from MedChemExpress. Usage Cited in: iScience. 2024 May 22;27(6):110075. [Abstract]
Representative microscopy images of H2DCFDA-stained HK-2 cells treated with 100 μM H2O2 alone or 100 μM H2O2 plus 100 nM Bradykinin to detect ROS.
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iScience
2022 Nov 8;25(11):105446. PMID: 36388955
Bradykinin purchased from MedChemExpress. Usage Cited in: iScience. 2022 Nov 8;25(11):105446. [Abstract]
Fluo-4 direct staining shows the Bradykinin (100 nM)-induced global Ca2+ transients in the KO myoblast versus CTR.
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ACS Chem Neurosci
2021 Jul 21;12(14):2619-2628. PMID: 34184863 -
J Am Soc Mass Spectrom
Wire Desorption Combined with Electrospray Ionization Mass Spectrometry: Direct Analysis of Small Organic and Large Biological Compounds. [Abstract]2020 Jul 5. PMID: 32559077 -
SLAS Discov
A New Model of Sensorial Neuron-Like Cells for HTS of Novel Analgesics for Neuropathic Pain. [Abstract]2019 Feb;24(2):158-168. PMID: 30383474 -
World J Surg Oncol
2019 Feb 27;17(1):44. PMID: 30813916
Bradykinin purchased from MedChemExpress. Usage Cited in: World J Surg Oncol. 2019 Feb 27;17(1):44. [Abstract]
H&E staining showed the flaps from rats treated with Bradykinin (150 μg/kg, i.p.) showed more neovascularization and less necrosis than those from the control group.
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Biochem Biophys Res Commun
Bradykinin attenuates NiSO4-induced autophagy in MIN6 cells and protects islet function in mice by regulating the PI3K/AKT/mTOR signaling pathway. [Abstract]2024 Dec 31:746:151265. PMID: 39756208 -
Rapid Commun Mass Spectrom
Two-step particle swarm optimization algorithm for effective deconvolution and resolution enhancement of various overlapping peaks. [Abstract]2023 Feb 15;37(3):e9429. PMID: 36346291 -
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Int Heart J
Decrease in Caveolae-Gαq Interaction Mediates Pressure Overload-Induced Cardiac Remodeling in Rats. [Abstract]2025 May 31;66(3):485-496. PMID: 40368774 -
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Solvent & Solubility
H2O : ≥ 100 mg/mL (94.32 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (94.32 mM); Clear solution; Need ultrasonic
Protocol
SW480 cells are pretreated with different concentrations of bradykinin (0, 0.1, 0.5, 1 μM), and then subjected to invasion and migration assays[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Rats: Bradykinin acetate is applied topically to the surface of the ovary with a thin piece of cotton (7×7 mm square) soaked with the solution. After application for 30 s, the cotton is removed. Each of the stimuli is delivered to the animal after observing stabilization of heart rate and mean arterial pressure[4].
Mouse: Mice are randomly divided into 3 groups: Sham, Model and Bradykinin. Before surgical procedure, the mice of bradykinin group are intraperitoneally injected with bradykinin (10 mg/kg/d) once a day for three days. After ligation, the mice receive bradykinin injection for another two days and analgesic therapy is performed using buprenorphine at 0.1 mg/kg body weight for 3 days. The mice in other groups receive equal saline as control[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (289 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Hornig B, et al. Role of bradykinin in mediating vascular effects of angiotensin-converting enzyme inhibitors inhumans. Circulation. 1997 Mar 4;95(5):1115-8. [Content Brief]
[2]. Wang G, et al. Bradykinin stimulates IL-6 production and cell invasion in colorectal cancer cells. Oncol Rep. 2014 Oct;32(4):1709-14. [Content Brief]
[3]. Dong R, et al. Exogenous Bradykinin Inhibits Tissue Factor Induction and Deep Vein Thrombosis via Activating the eNOS/Phosphoinositide 3-Kinase/Akt Signaling Pathway. Cell Physiol Biochem. 2015;37(4):1592-606. [Content Brief]
[4]. Uchida S, et al. Afferent fibers involved in the bradykinin-induced cardiovascular reflexes from the ovary in rats. Auton Neurosci. 2015 Dec;193:57-62. [Content Brief]
[5]. Liu LT, et al. Effect of vascular bradykinin on pancreatic microcirculation and hemorheology in rats with severe acute pancreatitis. Eur Rev Med Pharmacol Sci. 2015;19(14):2646-50. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O | 1 mM | 0.9432 mL | 4.7160 mL | 9.4321 mL | 23.5802 mL |
| 5 mM | 0.1886 mL | 0.9432 mL | 1.8864 mL | 4.7160 mL | |
| 10 mM | 0.0943 mL | 0.4716 mL | 0.9432 mL | 2.3580 mL | |
| 15 mM | 0.0629 mL | 0.3144 mL | 0.6288 mL | 1.5720 mL | |
| 20 mM | 0.0472 mL | 0.2358 mL | 0.4716 mL | 1.1790 mL | |
| 25 mM | 0.0377 mL | 0.1886 mL | 0.3773 mL | 0.9432 mL | |
| 30 mM | 0.0314 mL | 0.1572 mL | 0.3144 mL | 0.7860 mL | |
| 40 mM | 0.0236 mL | 0.1179 mL | 0.2358 mL | 0.5895 mL | |
| 50 mM | 0.0189 mL | 0.0943 mL | 0.1886 mL | 0.4716 mL | |
| 60 mM | 0.0157 mL | 0.0786 mL | 0.1572 mL | 0.3930 mL | |
| 80 mM | 0.0118 mL | 0.0590 mL | 0.1179 mL | 0.2948 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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