2. GPCR/G Protein Metabolic Enzyme/Protease
  3. Bradykinin Receptor Endogenous Metabolite Ser/Thr Protease
  4. Bradykinin

Bradykinin is an effective endothelium-dependent vasodilator that can lower blood pressure. Bradykinin can induce contraction of bronchial and intestinal non-vascular smooth muscle, increase vascular permeability, and participate in the mechanism of pain.

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Custom Peptide Synthesis

CAS No. : 58-82-2

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Based on 17 publication(s) in Google Scholar

Other Forms of Bradykinin:

Bradykinin Related Antibodies

Top Publications Citing Use of Products

    Bradykinin purchased from MedChemExpress. Usage Cited in: iScience. 2024 May 22;27(6):110075.

    Representative Western blot images of Nrf2, xCT and GPX4 expression in HK-2 cells treated with 100 μM H2O2 and different concentrations of Bradykinin (0, 10, 50, 100, 500, 1000 nM) for 24 h.

    Bradykinin purchased from MedChemExpress. Usage Cited in: iScience. 2024 May 22;27(6):110075.

    Flow cytometry of reactive oxygen species (ROS) in HK-2 cells treated with 100 μM H2O2 alone or 100 μM H2O2 plus 100 nM Bradykinin.

    Bradykinin purchased from MedChemExpress. Usage Cited in: iScience. 2024 May 22;27(6):110075.

    Representative microscopy images of H2DCFDA-stained HK-2 cells treated with 100 μM H2O2 alone or 100 μM H2O2 plus 100 nM Bradykinin to detect ROS.

    Bradykinin purchased from MedChemExpress. Usage Cited in: iScience. 2022 Nov 8;25(11):105446.

    Fluo-4 direct staining shows the Bradykinin (100 nM)-induced global Ca2+ transients in the KO myoblast versus CTR.

    Bradykinin purchased from MedChemExpress. Usage Cited in: World J Surg Oncol. 2019 Feb 27;17(1):44.  [Abstract]

    H&E staining showed the flaps from rats treated with Bradykinin (150 μg/kg, i.p.) showed more neovascularization and less necrosis than those from the control group.

    View All Bradykinin Receptor Isoform Specific Products:

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    Bradykinin B1 Receptor (B1R) Bradykinin B2 Receptor (B2R)

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    View All Isoforms
    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Bradykinin is an effective endothelium-dependent vasodilator that can lower blood pressure. Bradykinin can induce contraction of bronchial and intestinal non-vascular smooth muscle, increase vascular permeability, and participate in the mechanism of pain[1][2][3][4][5].

    IC50 & Target

    Bradykinin B2 Receptor (B2R)

     

    Human Endogenous Metabolite

     

    In Vitro

    Bradykinin is a potent vasodilator peptide that exerts its vasodilatory action through stimulation of specific endothelial B2 receptors, thereby causing the release of prostacyclin, NO, and EDHF[1]. Bradykinin has been reported to be involved in the progression of many types of cancer. Bradykinin treatment promotes the invasion and migration of colorectal cancer cells. Bradykinin treatment stimulates ERK1/2 activation and IL-6 production[2]. Exogenous bradykinin markedly inhibits TF expression in mRNA and protein level induced by LPS in a dose-dependent manner. The NO synthase antagonist L-NAME and PI3K inhibitor LY294002 dramatically abolish the inhibitory effects of bradykinin on tissue factor expression[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Bradykinin Related Antibodies
    In Vivo

    Application of 1 μM bradykinin to the ovary produces significant decreases in heart rate and mean arterial pressure. In vagotomized animals, application of 1 μM bradykinin to the ovary produces bradycardia and hypotension similar to the responses evoked when vagal innervation is intact[4].
    Vascular bradykinin can improve pancreatic microcirculation and hemorheology in rats with severe acute pancreatitis. The pancreatic microcirculatory blood flow volume and velocity in the vascular bradykinin treatment group increase gradually after 48 h[5].
    PI3K/Akt signaling pathway activation induced by bradykinin administration reduces the activity of GSK-3β and MAPK, and reduces NF-x03BA; B level in the nucleus, thereby inhibiting TF expression. Consistent with this, intraperitoneal injection of C57/BL6 mice with bradykinin also inhibits the thrombus formation induced by ligation of inferior vena cava[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Molecular Weight

    1060.21

    Formula

    C50H73N15O11
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    Sequence

    Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
    Sequence Shortening

    RPPGFSPFR
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Sealed storage, away from moisture

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 100 mg/mL (94.32 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.9432 mL 4.7160 mL 9.4321 mL
    5 mM 0.1886 mL 0.9432 mL 1.8864 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (94.32 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.98%

    SDS (252 KB)

    COA (263 KB) RP-HPLC (263 KB) MS (71 KB) Elemental Analysis Report (122 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [2]

    SW480 cells are pretreated with different concentrations of bradykinin (0, 0.1, 0.5, 1 μM), and then subjected to invasion and migration assays[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [3][4]

    Rats: Bradykinin acetate is applied topically to the surface of the ovary with a thin piece of cotton (7×7 mm square) soaked with the solution. After application for 30 s, the cotton is removed. Each of the stimuli is delivered to the animal after observing stabilization of heart rate and mean arterial pressure[4].

    Mouse: Mice are randomly divided into 3 groups: Sham, Model and Bradykinin. Before surgical procedure, the mice of bradykinin group are intraperitoneally injected with bradykinin (10 mg/kg/d) once a day for three days. After ligation, the mice receive bradykinin injection for another two days and analgesic therapy is performed using buprenorphine at 0.1 mg/kg body weight for 3 days. The mice in other groups receive equal saline as control[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 0.9432 mL 4.7160 mL 9.4321 mL 23.5802 mL
    5 mM 0.1886 mL 0.9432 mL 1.8864 mL 4.7160 mL
    10 mM 0.0943 mL 0.4716 mL 0.9432 mL 2.3580 mL
    15 mM 0.0629 mL 0.3144 mL 0.6288 mL 1.5720 mL
    20 mM 0.0472 mL 0.2358 mL 0.4716 mL 1.1790 mL
    25 mM 0.0377 mL 0.1886 mL 0.3773 mL 0.9432 mL
    30 mM 0.0314 mL 0.1572 mL 0.3144 mL 0.7860 mL
    40 mM 0.0236 mL 0.1179 mL 0.2358 mL 0.5895 mL
    50 mM 0.0189 mL 0.0943 mL 0.1886 mL 0.4716 mL
    60 mM 0.0157 mL 0.0786 mL 0.1572 mL 0.3930 mL
    80 mM 0.0118 mL 0.0590 mL 0.1179 mL 0.2948 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Bradykinin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Bradykinin58-82-2Bradykinin ReceptorEndogenous MetaboliteSer/Thr ProteaseSerine proteasesSerine endopeptidasesThreonine proteasesInhibitorinhibitorinhibit

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