1. Metabolic Enzyme/Protease
    Apoptosis
    Autophagy
  2. Phosphatase
    Apoptosis
    Autophagy
  3. CCT007093

CCT007093 

Cat. No.: HY-15880 Purity: ≥98.0%
Handling Instructions

CCT007093 is an effective protein phosphatase 1D (PPM1D Wip1) inhibitor. Wip1 inhibition can activate the mTORC1 pathway and enhance hepatocyte proliferation after hepatectomy.

For research use only. We do not sell to patients.

CCT007093 Chemical Structure

CCT007093 Chemical Structure

CAS No. : 176957-55-4

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 83 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 83 In-stock
Estimated Time of Arrival: December 31
Solid
10 mg USD 75 In-stock
Estimated Time of Arrival: December 31
50 mg USD 250 In-stock
Estimated Time of Arrival: December 31
100 mg USD 450 In-stock
Estimated Time of Arrival: December 31
500 mg USD 1250 Get quote
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Description

CCT007093 is an effective protein phosphatase 1D (PPM1D Wip1) inhibitor. Wip1 inhibition can activate the mTORC1 pathway and enhance hepatocyte proliferation after hepatectomy[1][2].

In Vitro

CCT007093 (25 or 50 µM, 8 h) significantly increases the phosphorylation levels of mTOR at Ser2448, Ser2481 and Ser2159, and the phosphorylation levels of p70S6K (Thr389) and S6 (Ser235/236) are also up-regulated in transfected HEK293T cell line[1].
CCT007093 shows specificity for MCF-7 cells, reducing viability by 40% after 2 days with no observable effect on the growth of HeLa cells[2].
CCT007093 induces P38 phosphorylation at 4 h post exposure in MCF-7 cells (sensitive to PPM1D inhibition), and does not induce P38 phosphorylation in HeLa cells (relatively resistant to PPM1D inhibition)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: HEK293T cell line.
Concentration: 25 or 50 µM.
Incubation Time: 8 h.
Result: The phosphorylation levels of mTOR at Ser2448, Ser2481 and Ser2159 were all significantly increased[1].
The phosphorylation levels of p70S6K (Thr389) and S6 (Ser235/236) were also up-regulated[1].
In Vivo

CCT007093 (6.4 mg/kg) enhances liver regeneration and increases the survival rate of mice following major hepatectomy[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Wild-type mice[1].
Dosage: 3.2 and 6.4 mg/kg.
Administration: Injected intraperitoneally 4 times (mice were sacrificed at 36 h post-PH).
Result: Significantly improved survival in mice following major hepatectomy (80% hepatectomy).
The level of PCNA was also significantly increased in the liver of CCT007093-treated mice at 36h, 48h and 72h post-PH.
Molecular Weight

272.39

Formula

C₁₅H₁₂OS₂

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMF : 3.33 mg/mL (12.23 mM; Need ultrasonic)

DMSO : ≥ 2.8 mg/mL (10.28 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6712 mL 18.3560 mL 36.7121 mL
5 mM 0.7342 mL 3.6712 mL 7.3424 mL
10 mM 0.3671 mL 1.8356 mL 3.6712 mL
*Please refer to the solubility information to select the appropriate solvent.
References
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Product Name:
CCT007093
Cat. No.:
HY-15880
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