Carnosol

Name: Carnosol
Brand: MedChemExpress
SKU: HY-N0643
Rating: 5.0 (16 reviews)

Cat. No.: HY-N0643 Purity: 98.79%: Data Sheet
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Carnosol is a potent Ribosomal S6 Kinase (RSK2) inhibitor that could be useful for treating gastric cancer, with an IC₅₀ of ~5.5 μM. Carnosol, a Nrf2 activator, increases the nuclear levels of Nrf2 and can promote the expression of heme oxygenase 1 (HMOX1).

For research use only. We do not sell to patients.

CAS No. : 5957-80-2

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Customer Review

Based on 16 publication(s) in Google Scholar

Other Forms of Carnosol:

Carnosol (Standard) Get quote

16 Publications Citing Use of MCE Carnosol

In Vivo Efficacy Study

Cell Imaging/Staining

: Carnosol purchased from MedChemExpress. Usage Cited in: Int J Surg. 2025 Jul 9. [Abstract]; Carnosol (200 mg/kg i.p. injection every 3 days). Combined application of CytoTRACE to dissect HMOX1_malignant+ and HMOX1_malignant− cells. (E) Tumor growth in ID8 tumor-bearing mice treated with saline, IgG, αPD-1, carnosol or αPD-1 + carnosol (n = 8 mice/group). Tumor growth was monitored by measuring the tumor volume for 2 weeks.

: Carnosol purchased from MedChemExpress. Usage Cited in: Int J Surg. 2025 Jul 9. [Abstract]; Carnosol (200 mg/kg i.p. injection every 3 days). Tumor growth in mice bearing ID8, Mice were given saline, IgG, αPD-1, carnosol, IMD0354, carnosol + αPD-1, IMD0354 + αPD-1 or carnosol + IMD0354 + αPD-1. N = 8 mice/group. Tumor growth was monitored by measuring the tumor volume for 2 weeks.

: Carnosol purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2025 Sep:190:118397. [Abstract]; Minced gastrocnemius muscles from six-month-old C57BL/6 male mice were mixed with ethanol (0.01 %; solvent; CTRL), BHT (positive control), carnosol (CO), rosmanol (ROS), isoromanol (ISOR), dimethylcarnosol (DCO) or dimethylisorosmanol (DISOR), all dissolved in ethanol and mixed at 0.01 % w/w minced muscle. At day 0 and day 7 of refrigerated storage (+4 °C), lipid oxidation was evaluated by TBARS quantification.

: Carnosol purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2025 Sep:190:118397. [Abstract]; Carnosol (CO, 48 h at 10 μM). Human myotubes treated for 36 h with 10 μM of carnosol analogues or DMSO (Solvent; CTRL) prior to incubation with sub-lethal concentration of 100 μM H2O2 for one hour. Western blot analysis of phosphorylated γH2AX protein level.

: Carnosol purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2025 Sep:190:118397. [Abstract]; Carnosol (CO, 48 h at 10 μM). Differentiated myoblasts were treated or not (CTRL; 0.1 % DMSO) with CO, ROS, ISOR, DCO and DISOR, at a concentration of 10 μM. Two days later, myotubes were processed for indirect immunofluorescence analysis with an antibody against troponin T (green). Nuclei were visualized by DAPI staining (blue). Representative images.

: Carnosol purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2025 Sep:190:118397. [Abstract]; Carnosol (CO, 48 h at 10 μM). In parallel, myotube protein extracts were analyzed by western blotting with anti-Muscle Ring-Finger Protein-1 (MuRF1; TRIM 63) antibody (green). Revert® total protein stain was used as internal loading control (red).

: Carnosol purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 28;13(7):653. [Abstract]; Survival analysis of the indicated mice in CLP-induced sepsis with or without treatment of 10 mg/kg HET0016, 20 mg/kg JSH-23, or 10 mg/kg Carnosol at 2 h before CLP and 12, 24, 48, and 72 h after CLP (n = 15 per group).

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Carnosol is a potent Ribosomal S6 Kinase (RSK2) inhibitor that could be useful for treating gastric cancer, with an IC₅₀ of ~5.5 μM^[1]. Carnosol, a Nrf2 activator, increases the nuclear levels of Nrf2 and can promote the expression of heme oxygenase 1 (HMOX1)^[2]^[3].

IC₅₀ & Target^[1]^[2]

RSK2

~5.5 μM (IC₅₀)

Nrf2

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	GI₅₀	3.6 μM Compound: 2	Cytotoxicity against human A2780 cells after 48 hrs by SRB assay Cytotoxicity against human A2780 cells after 48 hrs by SRB assay	[PMID: 17190465]
A549	IC₅₀	47 μM Compound: 1	Cytotoxicity against human A549 cells at exponential phase of growth after 48 hrs by MTT assay Cytotoxicity against human A549 cells at exponential phase of growth after 48 hrs by MTT assay	[PMID: 19711987]
A549	IC₅₀	5 μM Compound: 7	Inhibition of microsomal PGES1 in ILbeta-stimulated human A549 cells using PGH2 as substrate preincubated for 15 mins followed by substrate addition measured after 1 min by RP-HPLC analysis Inhibition of microsomal PGES1 in ILbeta-stimulated human A549 cells using PGH2 as substrate preincubated for 15 mins followed by substrate addition measured after 1 min by RP-HPLC analysis	10.1039/C5MD00278H
A549	IC₅₀	> 60 μM Compound: 1	Cytotoxicity against human A549 cells at lag phase of growth after 48 hrs by MTT assay Cytotoxicity against human A549 cells at lag phase of growth after 48 hrs by MTT assay	[PMID: 19711987]
HBL-100	GI₅₀	3.9 μM Compound: 2	Cytotoxicity against human HBL100 cells after 48 hrs by SRB assay Cytotoxicity against human HBL100 cells after 48 hrs by SRB assay	[PMID: 17190465]
HeLa	IC₅₀	43.9 μM Compound: 1	Cytotoxicity against human HeLa cells at lag phase of growth after 48 hrs by MTT assay Cytotoxicity against human HeLa cells at lag phase of growth after 48 hrs by MTT assay	[PMID: 19711987]
HeLa	IC₅₀	> 60 μM Compound: 1	Cytotoxicity against human HeLa cells at exponential phase of growth after 48 hrs by MTT assay Cytotoxicity against human HeLa cells at exponential phase of growth after 48 hrs by MTT assay	[PMID: 19711987]
MCF7	IC₅₀	40.9 μM Compound: 1	Cytotoxicity against human MCF7 cells at lag phase of growth after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells at lag phase of growth after 48 hrs by MTT assay	[PMID: 19711987]
MCF7	IC₅₀	44.5 μM Compound: 1	Cytotoxicity against human sMCF7 cells at exponential phase of growth after 48 hrs by MTT assay Cytotoxicity against human sMCF7 cells at exponential phase of growth after 48 hrs by MTT assay	[PMID: 19711987]
SW1573	GI₅₀	10 μM Compound: 2	Cytotoxicity against human SW1573 cells after 48 hrs by SRB assay Cytotoxicity against human SW1573 cells after 48 hrs by SRB assay	[PMID: 17190465]
T47D	GI₅₀	24 μM Compound: 2	Cytotoxicity against human T47D cells after 48 hrs by SRB assay Cytotoxicity against human T47D cells after 48 hrs by SRB assay	[PMID: 17190465]
Vero	IC₅₀	51.5 μM Compound: 1	Cytotoxicity against african green monkey Vero cells at exponential phase of growth after 48 hrs by MTT assay Cytotoxicity against african green monkey Vero cells at exponential phase of growth after 48 hrs by MTT assay	[PMID: 19711987]
Vero	IC₅₀	> 60 μM Compound: 1	Cytotoxicity against african green monkey Vero cells at lag phase of growth after 48 hrs by MTT assay Cytotoxicity against african green monkey Vero cells at lag phase of growth after 48 hrs by MTT assay	[PMID: 19711987]
WiDr	GI₅₀	26 μM Compound: 2	Cytotoxicity against human WiDr cells after 48 hrs by SRB assay Cytotoxicity against human WiDr cells after 48 hrs by SRB assay	[PMID: 17190465]

In Vitro

It is showed that carnosol has no cytotoxic effects on GES1 cells and 10 μM carnosol strongly suppresses RSK2 activity, but has little effect on any other kinase. Carnosol exerts strong dose-dependent inhibitory effects against RSK2 autophosphorylation and phosphorylation of its substrate ATF1^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Results indicate that Carnosol significantly decreaseS the volume and weight of gastric tumors relative to the vehicle-treated group. Additionally, mice tolerate treatment with carnosol without significant loss of body weight similar to the vehicle-treated group. The phosphorylation of CREB, a direct downstream protein of RSK2, Is strongly inhibited in the carnosol-treated group but the expression of total CREB is relatively unchanged^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

330.42

Formula

C₂₀H₂₆O₄

CAS No.

5957-80-2

Appearance

Solid

Color

White to off-white

SMILES

O=C1O[C@]2([H])C3=C(C(O)=C(O)C(C(C)C)=C3)[C@@]41CCCC(C)(C)[C@]4([H])C2

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (151.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0265 mL	15.1323 mL	30.2645 mL
5 mM	0.6053 mL	3.0265 mL	6.0529 mL
10 mM	0.3026 mL	1.5132 mL	3.0265 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (6.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*The compound is unstable in solutions, freshly prepared is recommended.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (280 KB) SDS (394 KB)

COA (253 KB) HNMR (205 KB) LCMS (121 KB)

Handling Instructions (2659 KB)

References

[1]. Wang L, et al. Carnosol suppresses patient-derived gastric tumor growth by targeting RSK2. Oncotarget. 2018 Feb 6;9(76):34200-34212. [Content Brief]

[2]. Li X, et al. Carnosol as a Nrf2 Activator Improves Endothelial Barrier Function Through Antioxidative Mechanisms. Int J Mol Sci. 2019;20(4):880. Published 2019 Feb 18. [Content Brief]

[3]. Martin D, et al. Regulation of heme oxygenase-1 expression through the phosphatidylinositol 3-kinase/Akt pathway and the Nrf2 transcription factor in response to the antioxidant phytochemical carnosol. J Biol Chem. 2004;279(10):8919-8929. [Content Brief]

Animal Administration
^[1]

Mice^[1]
Mice are orally administrated Carnosol at 100 mg/kg or vehicle 5 times a week over a period of 31 days^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Wang L, et al. Carnosol suppresses patient-derived gastric tumor growth by targeting RSK2. Oncotarget. 2018 Feb 6;9(76):34200-34212. [Content Brief]

[2]. Li X, et al. Carnosol as a Nrf2 Activator Improves Endothelial Barrier Function Through Antioxidative Mechanisms. Int J Mol Sci. 2019;20(4):880. Published 2019 Feb 18. [Content Brief]

[3]. Martin D, et al. Regulation of heme oxygenase-1 expression through the phosphatidylinositol 3-kinase/Akt pathway and the Nrf2 transcription factor in response to the antioxidant phytochemical carnosol. J Biol Chem. 2004;279(10):8919-8929. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.0265 mL	15.1323 mL	30.2645 mL	75.6613 mL
	5 mM	0.6053 mL	3.0265 mL	6.0529 mL	15.1323 mL
	10 mM	0.3026 mL	1.5132 mL	3.0265 mL	7.5661 mL
	15 mM	0.2018 mL	1.0088 mL	2.0176 mL	5.0441 mL
	20 mM	0.1513 mL	0.7566 mL	1.5132 mL	3.7831 mL
	25 mM	0.1211 mL	0.6053 mL	1.2106 mL	3.0265 mL
	30 mM	0.1009 mL	0.5044 mL	1.0088 mL	2.5220 mL
	40 mM	0.0757 mL	0.3783 mL	0.7566 mL	1.8915 mL
	50 mM	0.0605 mL	0.3026 mL	0.6053 mL	1.5132 mL
	60 mM	0.0504 mL	0.2522 mL	0.5044 mL	1.2610 mL
	80 mM	0.0378 mL	0.1892 mL	0.3783 mL	0.9458 mL
	100 mM	0.0303 mL	0.1513 mL	0.3026 mL	0.7566 mL

Carnosol Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Carnosol5957-80-2Ribosomal S6 Kinase (RSK)Keap1-Nrf2Endogenous MetaboliteS6KInhibitorinhibitorinhibit

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Carnosol

Customer Review

Other Forms of Carnosol:

Carnosol Related Antibodies

16 Publications Citing Use of MCE Carnosol

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View All Ribosomal S6 Kinase (RSK) Isoform Specific Products:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Carnosol Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Carnosol Related Classifications

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