2. Neuronal Signaling
  3. Cholinesterase (ChE)
  4. Donepezil Hydrochloride

Donepezil Hydrochloride  (Synonyms: E2020)

Cat. No.: HY-B0034 Purity: 99.92%
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Donepezil Hydrochloride (E2020) is a reversible, selective AChE inhibitor with an IC50 of 6.7 nM for AChE activity. Donepezil shows high selectivity for AChE over BuChE. Donepezil exhibits neuroprotective effect on Aβ42 neurotoxicity.

For research use only. We do not sell to patients.

CAS No. : 120011-70-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
25 mg In-stock
50 mg In-stock
100 mg In-stock
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Customer Review

Based on 24 publication(s) in Google Scholar

Other Forms of Donepezil Hydrochloride:

Donepezil Hydrochloride Related Antibodies

Top Publications Citing Use of Products

    Donepezil Hydrochloride purchased from MedChemExpress. Usage Cited in: Alzheimers Res Ther. 2025 Feb 14;17(1):43.  [Abstract]

    APP/PS1 mice were administered Donepezil Hydrochloride intraperitoneally at 1 mg/kg daily for three months to perform the Y-maze test.

    Donepezil Hydrochloride purchased from MedChemExpress. Usage Cited in: Alzheimers Res Ther. 2025 Feb 14;17(1):43.  [Abstract]

    APP/PS1 mice were administered Donepezil Hydrochloride intraperitoneally at 1 mg/kg daily for three months to test plateau quadrant dwell time.

    Donepezil Hydrochloride purchased from MedChemExpress. Usage Cited in: Alzheimers Res Ther. 2025 Feb 14;17(1):43.  [Abstract]

    APP/PS1 mice were administered Donepezil Hydrochloride intraperitoneally at 1 mg/kg daily for three months to test their plateau quadrant swimming distance.

    Donepezil Hydrochloride purchased from MedChemExpress. Usage Cited in: Alzheimers Res Ther. 2025 Feb 14;17(1):43.  [Abstract]

    APP/PS1 mice were intraperitoneally injected daily with Donepezil Hydrochloride at 1 mg/kg for 6E10 staining of the cortex and hippocampus for up to three months.

    Donepezil Hydrochloride purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jun 4:131:155802.  [Abstract]

    Donepezil (Don: 5 mg/kg). The latency of the mice swimming to the platform in MWM test.

    Donepezil Hydrochloride purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jun 4:131:155802.  [Abstract]

    Donepezil (Don: 5 mg/kg). Immunofluorescence staining of Brdu (red) and DCX (green) with DAPI (blue) in hippocampus.

    Donepezil Hydrochloride purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jun 4:131:155802.  [Abstract]

    Donepezil (Don: 5 mg/kg). Representative Western blot images.

    Donepezil Hydrochloride purchased from MedChemExpress. Usage Cited in: Eur J Med Chem. 2024 Feb 5:265:116071.  [Abstract]

    Effects of C7 and Donepezil (DZ, 5 mg/kg) on ​​AChE activity in mouse brain.

    Donepezil Hydrochloride purchased from MedChemExpress. Usage Cited in: Eur J Med Chem. 2024 Feb 5:265:116071.  [Abstract]

    Effects of C7 and Donepezil (DZ, 5 mg/kg) on ​​Aβ1-42-induced learning impairment in mice: spontaneous alternation behavior.

    Donepezil Hydrochloride purchased from MedChemExpress. Usage Cited in: Clin Transl Med. 2021 Jun;11(6):e428.

    Donepezil Hydrochloride at 1.0 or 2.0 mg/kg/day was injected intraperitoneally at 5 months after MS AAV‐CAG‐hTau‐mCherry‐3flag or AAV‐CAG‐vector‐mCherry‐3flag infusion. Both doses of Donepezil Hydrochloride rescued synaptic transmission in MShTau mice, as evidenced by increased input-output responses.

    Donepezil Hydrochloride purchased from MedChemExpress. Usage Cited in: Clin Transl Med. 2021 Jun;11(6):e428.

    Five months after infusion of MS AAV-CAG-hTau-mCherry-3flag or AAV-CAG-vector-mCherry-3flag, Donepezil Hydrochloride was administered intraperitoneally at doses of 1.0 or 2.0 mg/kg/day. Both doses of Donepezil Hydrochloride rescued synaptic transmission in MShTau mice, manifested as an increased fEPSP slope induced during LTP induction, and this increase remained significant 60 minutes after high-frequency stimulation (HFS).

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    Description

    Donepezil Hydrochloride (E2020) is a reversible, selective AChE inhibitor with an IC50 of 6.7 nM for AChE activity. Donepezil shows high selectivity for AChE over BuChE[1]. Donepezil exhibits neuroprotective effect on Aβ42 neurotoxicity[2].

    IC50 & Target

    AChE

     

    In Vitro

    Donepezil’s neuroprotective mechanism is related to the enhanced phosphorylation of Akt and GSK-3β and reduced phosphorylation of tau and glycogen synthase[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Donepezil Hydrochloride Related Antibodies

    Cell Viability Assay[2]

    Cell Line: Cortical neuronal cells
    Concentration: 0.01, 0.1, 1, and 10 μM
    Incubation Time: 24 hours
    Result: Exhibited significantly increased cell viability (maximized 89.2±2.1% in MTT, 96.3±5.5% in TBS, and 95.1±3.2% in CCK-8).

    Western Blot Analysis[2]

    Cell Line: Cortical neuronal cells
    Concentration: 10 μM
    Incubation Time: 24 hours before 20 μM Aβ42 exposure for 6 hours
    Result: Effects of Donepezil on Akt and the GSK-3 signaling pathway were statistically significant in the presence of Aβ42 toxicity.
    In Vivo

    Donepezil treatment (3 mg/kg) significantly prevents the progression of scopolamine-induced memory impairment in mice[3].
    A pharmacokinetic study of Donepezil shows a mean peak plasma concentration of donepezil after oral treatment (3 and 10 mg/kg) of approximately 1.2 h and 1.4 h, respectively; absolute bioavailability is calculated as 3.6%[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male imprinting control region (ICR) mice (6 weeks old)[3]
    Dosage: 3-10 mg/kg
    Administration: Administered orally
    Result: Pretreatment with 3–10 mg/kg ameliorated scopolamine-induced memory impairment.
    Animal Model: Hairless rats with an average weight of 300 g[3]
    Dosage: 3 and 10 mg/kg (Pharmacokinetic Analysis)
    Administration: Administered orally; and blood (250 μL) was collected through the tail vein
    Result: After oral treatment (3 and 10 mg/kg), a maximum concentration (Cmax) was reached after approximately 1.2 ± 0.4 h and 1.4 ± 0.5 h, respectively, and gradually decreased.
    Molecular Weight

    415.95

    Formula

    C24H30ClNO3
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C1C(CC2CCN(CC3=CC=CC=C3)CC2)CC4=C1C=C(OC)C(OC)=C4.[H]Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 25 mg/mL (60.10 mM; Need ultrasonic)

    DMSO : 6.2 mg/mL (14.91 mM; Need warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4041 mL 12.0207 mL 24.0414 mL
    5 mM 0.4808 mL 2.4041 mL 4.8083 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 1.25 mg/mL (3.01 mM); Clear solution

      This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 1.25 mg/mL (3.01 mM); Clear solution

      This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  Saline

      Solubility: 12.5 mg/mL (30.05 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.92%

    SDS (393 KB)

    COA (250 KB) LCMS (93 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 2.4041 mL 12.0207 mL 24.0414 mL 60.1034 mL
    5 mM 0.4808 mL 2.4041 mL 4.8083 mL 12.0207 mL
    10 mM 0.2404 mL 1.2021 mL 2.4041 mL 6.0103 mL
    H2O 15 mM 0.1603 mL 0.8014 mL 1.6028 mL 4.0069 mL
    20 mM 0.1202 mL 0.6010 mL 1.2021 mL 3.0052 mL
    25 mM 0.0962 mL 0.4808 mL 0.9617 mL 2.4041 mL
    30 mM 0.0801 mL 0.4007 mL 0.8014 mL 2.0034 mL
    40 mM 0.0601 mL 0.3005 mL 0.6010 mL 1.5026 mL
    50 mM 0.0481 mL 0.2404 mL 0.4808 mL 1.2021 mL
    60 mM 0.0401 mL 0.2003 mL 0.4007 mL 1.0017 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Donepezil Hydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Donepezil120011-70-3E2020E 2020E-2020Cholinesterase (ChE)AChENeuroprotectiveamyloid-βInhibitorinhibitorinhibit

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