Donepezil Hydrochloride
Based on 24 publication(s) in Google Scholar
Donepezil Hydrochloride (E2020) is a reversible, selective AChE inhibitor with an IC50 of 6.7 nM for AChE activity. Donepezil shows high selectivity for AChE over BuChE. Donepezil exhibits neuroprotective effect on Aβ42 neurotoxicity.
For research use only. We do not sell to patients.
- Purity: 99.92%
- CAS No.: 120011-70-3
- Formula: C24H30ClNO3
- Molecular Weight:415.95
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) Donepezil Hydrochloride
More- Cancer Cell. 2025 Aug 12:S1535-6108(25)00330-7. [Abstract]
- Phytomedicine. 2026 Jun:155:158102. [Abstract]
- Phytomedicine. 2025 Dec 1:150:157630. [Abstract]
- Phytomedicine. 2024 Jun 4:131:155802. [Abstract]
- Alzheimers Res Ther. 2025 Feb 14;17(1):43. [Abstract]
- Clin Transl Med. 2021 Jun;11(6):e428. [Abstract]
- Food Biosci. 2025 Sep.
- Eur J Med Chem. 2024 Feb 5:265:116071. [Abstract]
- J Ethnopharmacol. 2025 Apr 9:345:119619. [Abstract]
- J Ethnopharmacol. 2024 Nov 9;338(Pt 1):119017. [Abstract]
- Foods. 2025 May 29;14(11):1938. [Abstract]
- Foods. 2022 Jul 14;11(14):2095. [Abstract]
- Journal of Pharmaceutical Investigation. 2021 Mar 31.
- Int J Mol Sci. 2025 Apr 17;26(8):3781. [Abstract]
- Int Immunopharmacol. 2025 Mar 19:153:114442. [Abstract]
- Neuropharmacology. 2026 Jun 15:291:110923. [Abstract]
- Comput Struct Biotechnol J. 2023 Feb 24:21:1907-1920. [Abstract]
- Mol Brain. 2025 Apr 4;18(1):29. [Abstract]
- J Integr Neurosci. 2023 May 16;22(3):76. [Abstract]
- Chem Biodivers. 2025 Jan 7:e202402771. [Abstract]
- Neurol Res. 2025 Sep 13:1-18. [Abstract]
- bioRxiv. 2024 Dec 23:2024.12.22.629999. [Abstract]
- Research Square Preprint. 2024 Apr 23.
- Patent. US20210378961A1.
Customer Validation & Images
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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Histological Imaging/Staining
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In Vivo Efficacy Study
Biological Activity
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AChE |
Donepezil’s neuroprotective mechanism is related to the enhanced phosphorylation of Akt and GSK-3β and reduced phosphorylation of tau and glycogen synthase[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Cortical neuronal cells
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Concentration:0.01, 0.1, 1, and 10 μM
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Incubation Time:24 hours
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Result:Exhibited significantly increased cell viability (maximized 89.2±2.1% in MTT, 96.3±5.5% in TBS, and 95.1±3.2% in CCK-8).
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Cell Line:Cortical neuronal cells
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Concentration:10 μM
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Incubation Time:24 hours before 20 μM Aβ42 exposure for 6 hours
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Result:Effects of Donepezil on Akt and the GSK-3 signaling pathway were statistically significant in the presence of Aβ42 toxicity.
A pharmacokinetic study of Donepezil shows a mean peak plasma concentration of donepezil after oral treatment (3 and 10 mg/kg) of approximately 1.2 h and 1.4 h, respectively; absolute bioavailability is calculated as 3.6%[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male imprinting control region (ICR) mice (6 weeks old)[3]
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Dosage:3-10 mg/kg
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Administration:Administered orally
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Result:Pretreatment with 3–10 mg/kg ameliorated scopolamine-induced memory impairment.
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Animal Model:Hairless rats with an average weight of 300 g[3]
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Dosage:3 and 10 mg/kg (Pharmacokinetic Analysis)
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Administration:Administered orally; and blood (250 μL) was collected through the tail vein
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Result:After oral treatment (3 and 10 mg/kg), a maximum concentration (Cmax) was reached after approximately 1.2 ± 0.4 h and 1.4 ± 0.5 h, respectively, and gradually decreased.
Chemical Information
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CAS No. 120011-70-3
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Appearance Solid
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Molecular Weight 415.95
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Formula C24H30ClNO3
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Color White to off-white
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SMILES
O=C1C(CC2CCN(CC3=CC=CC=C3)CC2)CC4=C1C=C(OC)C(OC)=C4.[H]Cl
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Synonyms
E2020
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (24)
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Journal Impact Factor
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Most Recent
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Cancer Cell
2025 Aug 12:S1535-6108(25)00330-7. PMID: 40829591 -
Phytomedicine
Modified Kai-Xin-San ameliorates cognitive impairment in Alzheimer's disease model mice by regulating neuroinflammation and synaptic dysfunction. [Abstract]2026 Jun:155:158102. PMID: 41931996 -
Phytomedicine
Pterostilbene mitigates scopolamine-induced cognitive impairment by modulating cholinergic pathway and reducing neuroinflammation. [Abstract]2025 Dec 1:150:157630. PMID: 41353883 -
Phytomedicine
Zexieyin formula alleviates Alzheimer's disease via post-synaptic CaMKII modulating AMPA receptor: Involved in promoting neurogenesis to strengthen synaptic plasticity in mice hippocampus. [Abstract]2024 Jun 4:131:155802. PMID: 38852473
Donepezil Hydrochloride purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jun 4:131:155802. [Abstract]
Donepezil (Don: 5 mg/kg). The latency of the mice swimming to the platform in MWM test.
Donepezil Hydrochloride purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jun 4:131:155802. [Abstract]
Donepezil (Don: 5 mg/kg). Immunofluorescence staining of Brdu (red) and DCX (green) with DAPI (blue) in hippocampus.
Donepezil Hydrochloride purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Jun 4:131:155802. [Abstract]
Donepezil (Don: 5 mg/kg). Representative Western blot images.
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Alzheimers Res Ther
Pathway-based network medicine identifies novel natural products for Alzheimer's disease. [Abstract]2025 Feb 14;17(1):43. PMID: 39953559
Donepezil Hydrochloride purchased from MedChemExpress. Usage Cited in: Alzheimers Res Ther. 2025 Feb 14;17(1):43. [Abstract]
APP/PS1 mice were administered Donepezil Hydrochloride intraperitoneally at 1 mg/kg daily for three months to perform the Y-maze test.
Donepezil Hydrochloride purchased from MedChemExpress. Usage Cited in: Alzheimers Res Ther. 2025 Feb 14;17(1):43. [Abstract]
APP/PS1 mice were administered Donepezil Hydrochloride intraperitoneally at 1 mg/kg daily for three months to test plateau quadrant dwell time.
Donepezil Hydrochloride purchased from MedChemExpress. Usage Cited in: Alzheimers Res Ther. 2025 Feb 14;17(1):43. [Abstract]
APP/PS1 mice were administered Donepezil Hydrochloride intraperitoneally at 1 mg/kg daily for three months to test their plateau quadrant swimming distance.
Donepezil Hydrochloride purchased from MedChemExpress. Usage Cited in: Alzheimers Res Ther. 2025 Feb 14;17(1):43. [Abstract]
APP/PS1 mice were intraperitoneally injected daily with Donepezil Hydrochloride at 1 mg/kg for 6E10 staining of the cortex and hippocampus for up to three months.
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Clin Transl Med
Medial septum tau accumulation induces spatial memory deficit via disrupting medial septum-hippocampus cholinergic pathway. [Abstract]2021 Jun;11(6):e428. PMID: 34185417
Donepezil Hydrochloride purchased from MedChemExpress. Usage Cited in: Clin Transl Med. 2021 Jun;11(6):e428. [Abstract]
Donepezil Hydrochloride at 1.0 or 2.0 mg/kg/day was injected intraperitoneally at 5 months after MS AAV‐CAG‐hTau‐mCherry‐3flag or AAV‐CAG‐vector‐mCherry‐3flag infusion. Both doses of Donepezil Hydrochloride rescued synaptic transmission in MShTau mice, as evidenced by increased input-output responses.
Donepezil Hydrochloride purchased from MedChemExpress. Usage Cited in: Clin Transl Med. 2021 Jun;11(6):e428. [Abstract]
Five months after infusion of MS AAV-CAG-hTau-mCherry-3flag or AAV-CAG-vector-mCherry-3flag, Donepezil Hydrochloride was administered intraperitoneally at doses of 1.0 or 2.0 mg/kg/day. Both doses of Donepezil Hydrochloride rescued synaptic transmission in MShTau mice, manifested as an increased fEPSP slope induced during LTP induction, and this increase remained significant 60 minutes after high-frequency stimulation (HFS).
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Eur J Med Chem
Design, synthesis and biological evaluation of carbamate derivatives incorporating multifunctional carrier scaffolds as pseudo-irreversible cholinesterase inhibitors for the treatment of Alzheimer's disease. [Abstract]2024 Feb 5:265:116071. PMID: 38157596
Donepezil Hydrochloride purchased from MedChemExpress. Usage Cited in: Eur J Med Chem. 2024 Feb 5:265:116071. [Abstract]
Effects of C7 and Donepezil (DZ, 5 mg/kg) on AChE activity in mouse brain.
Donepezil Hydrochloride purchased from MedChemExpress. Usage Cited in: Eur J Med Chem. 2024 Feb 5:265:116071. [Abstract]
Effects of C7 and Donepezil (DZ, 5 mg/kg) on Aβ1-42-induced learning impairment in mice: spontaneous alternation behavior.
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J Ethnopharmacol
Lancao decoction alleviates Alzheimer's disease: Depending on activating CaMKII to protect neuronal refunction by reducing β-amyloid in the hippocampus. [Abstract]2025 Apr 9:345:119619. PMID: 40074096 -
J Ethnopharmacol
Lancao decoction in the treatment of alzheimer's disease via activating PI3K/AKT signaling to promote ERK involving in enhancing neuronal activities in the hippocampus. [Abstract]2024 Nov 9;338(Pt 1):119017. PMID: 39528121 -
Foods
Sea Cucumber Hydrolysates Alleviate Cognitive Deficits in D-Galactose-Induced C57BL/6J Aging Mice Associated with Modulation of Gut Microbiota. [Abstract]2025 May 29;14(11):1938. PMID: 40509464 -
Foods
Improving Effect of the Policosanol from Ericerus pela Wax on Learning and Memory Impairment Caused by Scopolamine in Mice. [Abstract]2022 Jul 14;11(14):2095. PMID: 35885338 -
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Int J Mol Sci
2025 Apr 17;26(8):3781. PMID: 40332446 -
Int Immunopharmacol
Farrerol alleviates cognitive impairments in chronic cerebral hypoperfusion via suppressing NLRP3 inflammasome-mediated pyroptosis. [Abstract]2025 Mar 19:153:114442. PMID: 40112599 -
Neuropharmacology
Ajugol ameliorates mitochondrial dysfunction and cognitive decline in Alzheimer's Disease via BNIP3-dependent mitophagy. [Abstract]2026 Jun 15:291:110923. PMID: 41819485 -
Comput Struct Biotechnol J
Network Proximity-based computational pipeline identifies drug candidates for different pathological stages of Alzheimer's disease. [Abstract]2023 Feb 24:21:1907-1920. PMID: 36936813 -
Mol Brain
Target oxidative stress-induced disulfidptosis: novel therapeutic avenues in Parkinson's disease. [Abstract]2025 Apr 4;18(1):29. PMID: 40186271 -
J Integr Neurosci
2023 May 16;22(3):76. PMID: 37258429 -
Chem Biodivers
Protocatechuic Acid Improves Alzheimer's Disease by Regulating the Cholinergic Synaptic Signaling Pathway. [Abstract]2025 Jan 7:e202402771. PMID: 39776239 -
Neurol Res
Polygonatum sibiricum polysaccharide ameliorates neurotoxicity in Alzheimer disease mice by inhibiting endoplasmic reticulum stress and lipid raft formation through AMPK/GSK3β/Nrf2 pathway. [Abstract]2025 Sep 13:1-18. PMID: 40946159 -
bioRxiv
In vivo multiplex imaging of dynamic neurochemical networks with designed far-red dopamine sensors. [Abstract]2024 Dec 23:2024.12.22.629999. PMID: 39763912 -
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Solvent & Solubility
H2O : 25 mg/mL (60.10 mM; Need ultrasonic)
DMSO : 6.2 mg/mL (14.91 mM; Need warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (3.01 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (3.01 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: Saline
Solubility: 12.5 mg/mL (30.05 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. H Ogura, et al. Comparison of inhibitory activities of donepezil and other cholinesterase inhibitors on acetylcholinesterase and butyrylcholinesterase in vitro. Methods Find Exp Clin Pharmacol. 2000 Oct;22(8):609-13. [Content Brief]
[2]. Min-Young Noh,et al. Neuroprotective effects of donepezil through inhibition of GSK-3 activity in amyloid-beta-induced neuronal cell death. J Neurochem. 2009 Mar;108(5):1116-25. [Content Brief]
[3]. Chang Yell Shin, et al. The Effects of Donepezil, an Acetylcholinesterase Inhibitor, on Impaired Learning and Memory in Rodents. Biomol Ther (Seoul). 2018 May 1;26(3):274-281. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 2.4041 mL | 12.0207 mL | 24.0414 mL | 60.1034 mL |
| 5 mM | 0.4808 mL | 2.4041 mL | 4.8083 mL | 12.0207 mL | |
| 10 mM | 0.2404 mL | 1.2021 mL | 2.4041 mL | 6.0103 mL | |
| H2O | 15 mM | 0.1603 mL | 0.8014 mL | 1.6028 mL | 4.0069 mL |
| 20 mM | 0.1202 mL | 0.6010 mL | 1.2021 mL | 3.0052 mL | |
| 25 mM | 0.0962 mL | 0.4808 mL | 0.9617 mL | 2.4041 mL | |
| 30 mM | 0.0801 mL | 0.4007 mL | 0.8014 mL | 2.0034 mL | |
| 40 mM | 0.0601 mL | 0.3005 mL | 0.6010 mL | 1.5026 mL | |
| 50 mM | 0.0481 mL | 0.2404 mL | 0.4808 mL | 1.2021 mL | |
| 60 mM | 0.0401 mL | 0.2003 mL | 0.4007 mL | 1.0017 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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