1. Apoptosis
  2. Apoptosis
  3. INH1

INH1 (Synonyms: IBT13131)

Cat. No.: HY-16660 Purity: 99.62%
Handling Instructions

INH1 specifically disrupts the Hec1/Nek2 interaction via direct Hec1 binding. INH1 shows promising cancer inhibition activity both in vitro and in vivo.

For research use only. We do not sell to patients.

INH1 Chemical Structure

INH1 Chemical Structure

CAS No. : 313553-47-8

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 73 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 73 In-stock
Estimated Time of Arrival: December 31
Solid
10 mg USD 66 In-stock
Estimated Time of Arrival: December 31
50 mg USD 220 In-stock
Estimated Time of Arrival: December 31
100 mg USD 385 In-stock
Estimated Time of Arrival: December 31
500 mg USD 1540 In-stock
Estimated Time of Arrival: December 31
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5 g   Get quote  

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Description

INH1 specifically disrupts the Hec1/Nek2 interaction via direct Hec1 binding. INH1 shows promising cancer inhibition activity both in vitro and in vivo[1].

In Vitro

INH1 (25 μM, 24 h) treatment resulted in reduced association of Hec1 with kinetochore and decrease of global Nek2 protein level[1].
INH1 exhibits GI50 values of 10.5 μM (in MDA-MB-468 cells), 15 μM (in SKBR3 cells), 10.5 μM (in T47D cells), 20.5 μM (in MDA-MB-361 cells), 15 μM (in ZR-75-1 cells), 15 μM (in HBL 100 cells), 15.5 μM (in MDA-MB-435 cells), 11 μM (in HS578T cells) and 41 μM (in MCF10A cells), respectively[1].
INH1 (5k) has an IC50 value of 176 nM in the dose-dependent transwell migration assays in MDA-MB-231 cells. INH1 (5k) substantially reduces cellular f-actin and prevented localization of fascin to actin-rich membrane protrusions[2].
INH1 induces abnormal mitotic processes, as well as cell apoptosis[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay[1]

Cell Line: MCF10A cells.
Concentration: 10 μM.
Incubation Time: 12 days.
Result: Effectively inhibits the proliferation of human breast cancer lines.

Western Blot Analysis[1]

Cell Line: MCF10A cells.
Concentration: 25 μM.
Incubation Time: 24 h.
Result: Nek2 reduction in INH1-treated cells may be independent of Hec1.
In Vivo

INH1 (50 or 100 mg/kg, i.p., every other day/25 cycles) inhibits tumor outgrowth in a xenografted breast cancer model in nude mice[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Xenografted nude mice breast cancer model[1].
Dosage: 50 or 100 mg/kg.
Administration: I.P., every other day/25 cycles.
Result: Inhibited tumor growth.
Molecular Weight

308.40

Formula

C18H16N2OS

CAS No.
SMILES

O=C(NC1=NC(C2=CC=C(C)C=C2C)=CS1)C3=CC=CC=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (324.25 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2425 mL 16.2127 mL 32.4254 mL
5 mM 0.6485 mL 3.2425 mL 6.4851 mL
10 mM 0.3243 mL 1.6213 mL 3.2425 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (8.11 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (8.11 mM); Clear solution

*All of the co-solvents are available by MCE.
References
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Product Name:
INH1
Cat. No.:
HY-16660
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