ML355
Based on 8 publication(s) in Google Scholar
ML355 is a potent and selective inhibitor of 12-Lipoxygenase (12-LOX) with an IC50 of 0.34 μM, shows excellent selectivity over related lipoxygenases and cyclooxygenases, and possesses favorable ADME properties.
For research use only. We do not sell to patients.
- Purity: 98.11%
- CAS No.: 1532593-30-8
- Formula: C21H19N3O4S2
- Molecular Weight:441.52
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) ML355
More- Nat Med. 2018 Jan;24(1):73-83. [Abstract]
- Cell Metab. 2021 Oct 5;33(10):2059-2075.e10. [Abstract]
- Nat Commun. 2024 Sep 10;15(1):7914. [Abstract]
- Cell Commun Signal. 2020 May 4;18(1):70. [Abstract]
- Phytomedicine. 2024 Jul 25:130:155757. [Abstract]
- Free Radic Biol Med. 2024 Oct:223:325-340. [Abstract]
- Int Immunopharmacol. 2024 Oct 7;143(Pt 1):113320. [Abstract]
- Inflammation. 2026 Mar 3;49(1):92. [Abstract]
Customer Validation & Images
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Others
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Flow Cytometry
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WB
Biological Activity
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12-LOX 0.34 μM (IC50) |
ML355 inhibits PAR-4 induced aggregation and calcium mobilization in human platelets and reduce 12-HETE in β-cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 mice[3]
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Dosage:1.88, 3.75, 7.5, 15, 30 mg/kg
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Administration:Oral gavage; 2 times per day for two days
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Result:The thrombus formation in mice was strongly inhibited by higher doses of ML355.
Chemical Information
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CAS No. 1532593-30-8
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Appearance Solid
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Molecular Weight 441.52
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Formula C21H19N3O4S2
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Color White to gray
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SMILES
O=S(NC1=NC2=CC=CC=C2S1)(C3=CC=C(NCC4=CC=CC(OC)=C4O)C=C3)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 1 year -20°C 6 months
Publications (8)
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Journal Impact Factor
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Most Recent
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Nat Med
An ALOX12-12-HETE-GPR31 signaling axis is a key mediator of hepatic ischemia-reperfusion injury. [Abstract]2018 Jan;24(1):73-83. PMID: 29227475
ML355 purchased from MedChemExpress. Usage Cited in: Nat Med. 2018 Jan;24(1):73-83. [Abstract]
The phosphorylated and total levels of p65, JNK, p38 and ERK in primary hepatocytes treated with ML355 (10 μM) under normoxia (Nor) or hypoxia (Hyp) conditions. For western blot analysis, GAPDH served as a loading control.
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Cell Metab
Pharmacological inhibition of arachidonate 12-lipoxygenase ameliorates myocardial ischemia-reperfusion injury in multiple species. [Abstract]2021 Oct 5;33(10):2059-2075.e10. PMID: 34536344 -
Nat Commun
A mouse protozoan boosts antigen-specific mucosal IgA responses in a specific lipid metabolism- and signaling-dependent manner. [Abstract]2024 Sep 10;15(1):7914. PMID: 39256385
ML355 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Sep 10;15(1):7914. [Abstract]
Starting from day 6, mice were administered the carrier and ML-355 (15 mg/kg) daily via oral gavage. On day 7, mice were challenged by intraperitoneal injection of OVA+CFA. On day 21, the relative levels of anti-OVA IgA in serum and cecal contents were measured.
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Cell Commun Signal
Cytochrome P450 1A1 enhances inflammatory responses and impedes phagocytosis of bacteria in macrophages during sepsis. [Abstract]2020 May 4;18(1):70. PMID: 32366266 -
Phytomedicine
Baicalein alleviates cisplatin-induced acute kidney injury by inhibiting ALOX12-dependent ferroptosis. [Abstract]2024 Jul 25:130:155757. PMID: 38805781 -
Free Radic Biol Med
CX-5461 ameliorates disease in lupus-prone mice by triggering B-cell ferroptosis via p53-SLC7A11-ALOX12 pathway. [Abstract]2024 Oct:223:325-340. PMID: 39111584
ML355 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Oct:223:325-340. [Abstract]
Selected Raji cells were treated with 1 μM CX-5461, with or without 10 μM ML355, and lipid ROS levels were analyzed by flow cytometry. Representative results and quantitative analyses are presented.
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Int Immunopharmacol
Palmitic acid inhibits macrophage-mediated chemotherapy resistance in multiple myeloma via ALOX12 signaling. [Abstract]2024 Oct 7;143(Pt 1):113320. PMID: 39378653 -
Inflammation
MAGL Inhibition Relieves Psoriasiform Inflammation and Pruritus Via Modulation of ALOX12-12-HETE Axis in Mice. [Abstract]2026 Mar 3;49(1):92. PMID: 41774275
Solvent & Solubility
DMSO : ≥ 42 mg/mL (95.13 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.66 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.66 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Luci DK, et al. Synthesis and structure-activity relationship studies of 4-((2-hydroxy-3-methoxybenzyl)amino)benzenesulfonamide derivatives as potent and selective inhibitors of 12-lipoxygenase. J Med Chem. 2014 Jan 23;57(2):495-506. [Content Brief]
[2]. Zhang XJ, et al. An ALOX12-12-HETE-GPR31 signaling axis is a key mediator of hepatic ischemia-reperfusion injury. Nat Med. 2018 Jan;24(1):73-83. [Content Brief]
[3]. Adili R, et al. First Selective 12-LOX Inhibitor, ML355, Impairs Thrombus Formation and Vessel Occlusion In Vivo With Minimal Effects on Hemostasis. Arterioscler Thromb Vasc Biol. 2017 Oct;37(10):1828-1839. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.2649 mL | 11.3245 mL | 22.6490 mL | 56.6226 mL |
| 5 mM | 0.4530 mL | 2.2649 mL | 4.5298 mL | 11.3245 mL | |
| 10 mM | 0.2265 mL | 1.1325 mL | 2.2649 mL | 5.6623 mL | |
| 15 mM | 0.1510 mL | 0.7550 mL | 1.5099 mL | 3.7748 mL | |
| 20 mM | 0.1132 mL | 0.5662 mL | 1.1325 mL | 2.8311 mL | |
| 25 mM | 0.0906 mL | 0.4530 mL | 0.9060 mL | 2.2649 mL | |
| 30 mM | 0.0755 mL | 0.3775 mL | 0.7550 mL | 1.8874 mL | |
| 40 mM | 0.0566 mL | 0.2831 mL | 0.5662 mL | 1.4156 mL | |
| 50 mM | 0.0453 mL | 0.2265 mL | 0.4530 mL | 1.1325 mL | |
| 60 mM | 0.0377 mL | 0.1887 mL | 0.3775 mL | 0.9437 mL | |
| 80 mM | 0.0283 mL | 0.1416 mL | 0.2831 mL | 0.7078 mL |
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