1. Protein Tyrosine Kinase/RTK
  2. FGFR

PD-166866 

Cat. No.: HY-101296 Purity: 99.68%
Handling Instructions

PD166866 is a selective FGFR1 tyrosine kinase inhibitor with an IC50 of 52.4 nM.

For research use only. We do not sell to patients.

PD-166866 Chemical Structure

PD-166866 Chemical Structure

CAS No. : 192705-79-6

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 77 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
5 mg USD 70 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
10 mg USD 110 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
25 mg USD 240 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
50 mg USD 420 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
100 mg USD 740 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

PD166866 is a selective FGFR1 tyrosine kinase inhibitor with an IC50 of 52.4 nM.

IC50 & Target[1]

FGFR1

52.4 nM (IC50)

In Vitro

PD 166866 inhibits human full-length FGFR-1 tyrosine kinase with an IC50 value of 52.4 nM and is characterized as an ATP competitive inhibitor of the FGFR-1. PD 166866 is a potent inhibitor of FGFR autophosphorylation in NIH 3T3 cells expressing endogenous FGFR-1 and in L6 cells overexpressing the human FGFR-1 tyrosine kinase. PD 166866 also inhibits bFGF-induced tyrosine phosphorylation of the 44- and 42-kDa (ERK 1/2) mitogen-activated protein kinase isoforms in L6 cells. Daily exposure of PD 166866 to L6 cells at concentrations from 1 to 100 nM results in a concentration-related inhibition of bFGF-stimulated cell growth for 8 consecutive days with an IC50 value of 24 nM[1].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 2.5224 mL 12.6122 mL 25.2245 mL
5 mM 0.5045 mL 2.5224 mL 5.0449 mL
10 mM 0.2522 mL 1.2612 mL 2.5224 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay
[1]

PD 166866 is dissolved in DMSO. PD 166866 or vehicle (0.5% DMSO, final concentration) are added every day to triplicate cultures of cells together with 25 ng/mL bFGF to stimulate FGF-driven growth. In some experiments, PD 166866 is added every day to triplicate cultures of cells together with 30 ng/mL PDGF-BB to stimulate PDGF-driven growth. Cell number is measured by Coulter counting on days 1, 3, 6 or 8 after drug exposure[1] MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

396.44

Formula

C₂₀H₂₄N₆O₃

CAS No.

192705-79-6

SMILES

O=C(NC(C)(C)C)NC1=NC2=NC(N)=NC=C2C=C1C3=CC(OC)=CC(OC)=C3

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: 10.33 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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Product Name:
PD-166866
Cat. No.:
HY-101296
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