1. Phenylpropanoids

Praeruptorin B (Synonyms: Praeruptorin D)

Cat. No.: HY-N0082
Handling Instructions

Praeruptorin B is an inhibitor of sterol regulatory element-binding proteins (SREBPs).

For research use only. We do not sell to patients.

Praeruptorin B Chemical Structure

Praeruptorin B Chemical Structure

CAS No. : 73069-28-0

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 165 In-stock
Estimated Time of Arrival: December 31
5 mg USD 150 In-stock
Estimated Time of Arrival: December 31
10 mg USD 240 In-stock
Estimated Time of Arrival: December 31
25 mg USD 480 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Praeruptorin B is an inhibitor of sterol regulatory element-binding proteins (SREBPs).

IC50 & Target

SREBP[1].

In Vitro

Praeruptorin B inhibits the SREBPs activity and decreases intracellular lipid levels. Praeruptorin B is found to powerfully decrease the SRE-luciferase activity, and this effect is dose dependent. Praeruptorin B shows negligible cytotoxicity, even at the higher concentration. Praeruptorin B also significantlytly down-regulates the expression of SREBP-1c and SREBP-2[1]. Praeruptorin B also exhibits significant inhibition on the activity of UGT1A9[2].

In Vivo

The mice treated with Praeruptorin B (50 mg/kg) are significantly lighter than the vehicle treated mice, although they are still heavier than the chow diet-fed mice, suggesting that Praeruptorin B can ameliorate diet-induced obesity (DIO). More importantly, the fat/lean and fat/body-weight ratios are obviously dropped at the same dosage of Praeruptorin B treated mice. It is also showed that the serum TC and TG levels of Praeruptorin B treated mice are significantly lower than those of the HFD-fed mice. Praeruptorin B increases HDL-c and decreases LDL-c similar as lovastatin. In addition, compared with vehicle treated mice, Praeruptorin B significantly lowers the level of TC and TG in liver, comparable to lovastatin. The staining results reveal that Praeruptorin B-treated mice exhibit less lipid accumulation than that of vehicle treated mice. The elevated fasting blood glucose and insulin in HFD-fed mice are significantly reduced by Praeruptorin B[1].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3449 mL 11.7244 mL 23.4489 mL
5 mM 0.4690 mL 2.3449 mL 4.6898 mL
10 mM 0.2345 mL 1.1724 mL 2.3449 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Praeruptorin B is dissolved in 0.5% CMC-Na for 6 weeks[1].

References
Cell Assay
[1]

HepG2 cells and HL-7702 cells are used in the study. Cell proliferation is determined by the MTT assays. The HepG2 cells are seeded in 96-well plates with 2.0×104 cells per well in DMEM containing 10% FBS for 24 h. Cells are further treated with Praeruptorin B (0, 2.5, 5, 10, 20, 40, 80 μM) for 18 h[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Mice[1]
Sixweek-old male C57BL/6J mice are housed in colony cages and maintained on a light/dark cycle. On a caloric basis, the HFD contains 60% fat, 20.6% carbohydrate and 19.4% protein, whereas the normal diet contains 13% fat, 60% carbohydrate and 27% protein. The mice are randomly divided into the following four groups (n=6 per group): vehicle-treated chow group, vehicle-treated HFD group, lovastatin-treated HFD group (30 mg per kg per day) and Praeruptorin B-treated HFD group (25 or 50 mg per kg per day). HFD-fed mice are gavaged with Praeruptorin B or lovastatin dissolved in 0.5% CMC-Na for 6 weeks[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

426.46

Formula

C₂₄H₂₆O₇

CAS No.

73069-28-0

SMILES

O=C1C=CC2=CC=C3C([[email protected]](OC(/C(C)=C\C)=O)[[email protected]](OC(/C(C)=C\C)=O)C(C)(C)O3)=C2O1

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
Praeruptorin B
Cat. No.:
HY-N0082
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Praeruptorin B

Cat. No.: HY-N0082