1. PI3K/Akt/mTOR
  2. mTOR

WAY-600 

Cat. No.: HY-15272 Purity: 95.41%
Data Sheet SDS Handling Instructions

WAY-600 is a potent, ATP-competitive, and selective mTOR inhibitor with an IC50 of 9 nM for recombinant mTOR enzyme.

For research use only. We do not sell to patients.
WAY-600 Chemical Structure

WAY-600 Chemical Structure

CAS No. : 1062159-35-6

Size Price Stock Quantity
10 mM * 1 mL in DMSO $207 In-stock
2 mg $110 In-stock
5 mg $190 In-stock
10 mg $260 In-stock
50 mg $690 In-stock
100 mg $1100 In-stock
200 mg   Get quote  
500 mg   Get quote  

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

WAY-600 is a potent, ATP-competitive, and selective mTOR inhibitor with an IC50 of 9 nM for recombinant mTOR enzyme.

IC50 & Target

IC50: 9 nM (mTOR)[1]

In Vitro

WAY-600 exhibits a concentration-dependent and time-dependent inhibition of f HepG2 and Huh-7 cells viability. Following WAY-600 (1-1000 nM) treatment, the number of HepG2 cell colonies is dramatically decreased. Meanwhile, BrdU incorporation in HepG2 cells is also inhibited with WAY-600 treatment. WAY-600 dose-dependently increases the activity of caspase-3 and caspase-9 in HepG2 cells. WAY-600 disrupts assemble of mTORC1 (mTOR-Raptor association) and mTORC2 (mTOR-Rictor association). Activation of mTORC1 (indicated by p-S6K1 and p-4E-BP1) and mTORC2 is almost blocked by WAY-600 (100 nM)[2].

In Vivo

Administration of WAY-600 (10 mg/kg, daily) inhibits HepG2 tumor growth in nude mice. Daily HepG2 tumor growth of WAY-600-administrated mice is significantly lower than that of vehicle control mice. Importantly, the in vivo anti-cancer activity by WAY-600 is further potentiated with the co-administration of MEK-162 (2.5 mg/kg, p.o. daily)[2].

References
Preparing Stock Solutions
Concentration Volume (DMSO) Mass 1 mg 5 mg 10 mg
1 mM 2.0219 mL 10.1094 mL 20.2188 mL
5 mM 0.4044 mL 2.0219 mL 4.0438 mL
10 mM 0.2022 mL 1.0109 mL 2.0219 mL
Cell Assay
[2]

WAY-600 is prepared in DMSO.

Established HCC cells (HepG2 and Huh-7), primary HCC cells (Pnt-1/-2/-3/-4), or THLE-2 liver cells are cultured in WAY-600 (1-1000 nM)-containing medium for 24, 48, 72, 96 hours, cell viability is tested by MTT assay[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2]

WAY-600 is prepared in 5% ethanol, 5% polysorbate 80, 5% polyethylene glycol-400.

Mouse: Mice tumor xenografts are randomly divided into four groups (10 mice per group): vehicle ( intraperitoneal or i.p.), WAY-600 (10 mg/kg, i.p. injection), MEK-162 (2.5 mg/kg, oral gavage) or WAY-600 plus MEK-162 combination. The mice are monitored for activity and physical condition on daily basis, and mice body weights and tumor mass are measured weekly[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

494.59

Formula

C₂₈H₃₀N₈O

CAS No.

1062159-35-6

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: 10 mM

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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Product Name:
WAY-600
Cat. No.:
HY-15272
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