1. PI3K/Akt/mTOR
  2. mTOR

WAY-600 

Cat. No.: HY-15272 Purity: 95.41%
Data Sheet SDS Handling Instructions

WAY-600 is a potent, ATP-competitive, and selective mTOR inhibitor with an IC50 of 9 nM for recombinant mTOR enzyme.

For research use only. We do not sell to patients.
WAY-600 Chemical Structure

WAY-600 Chemical Structure

CAS No. : 1062159-35-6

Size Price Stock Quantity
10 mM * 1 mL in DMSO $207 In-stock
2 mg $110 In-stock
5 mg $190 In-stock
10 mg $260 In-stock
50 mg $690 In-stock
100 mg $1100 In-stock
200 mg   Get quote  
500 mg   Get quote  

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    WAY-600 purchased from MCE. Usage Cited in: Biochem Biophys Res Commun. 2015 Oct 23;466(3):547-53.

    DNA-PKcs Thr-2609 phosphorylation is important for WAY-600 resistance in HT-29 cells. Expression of listed proteins in stable HT-29 cells expressing empty vector (pSV2 neo), Flag-tagged wild-type DNA-PKcs (wt-DNA-PKcs-Flag), Flag-tagged dominant negative DNA-PKcs (dn-DNA-PKcs-Flag, T2609A), or Flag-tagged wild-type PP5 (PP5-Flag), is tested by Western blots.

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    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    WAY-600 is a potent, ATP-competitive, and selective mTOR inhibitor with an IC50 of 9 nM for recombinant mTOR enzyme.

    IC50 & Target

    IC50: 9 nM (mTOR)[1]

    In Vitro

    WAY-600 exhibits a concentration-dependent and time-dependent inhibition of f HepG2 and Huh-7 cells viability. Following WAY-600 (1-1000 nM) treatment, the number of HepG2 cell colonies is dramatically decreased. Meanwhile, BrdU incorporation in HepG2 cells is also inhibited with WAY-600 treatment. WAY-600 dose-dependently increases the activity of caspase-3 and caspase-9 in HepG2 cells. WAY-600 disrupts assemble of mTORC1 (mTOR-Raptor association) and mTORC2 (mTOR-Rictor association). Activation of mTORC1 (indicated by p-S6K1 and p-4E-BP1) and mTORC2 is almost blocked by WAY-600 (100 nM)[2].

    In Vivo

    Administration of WAY-600 (10 mg/kg, daily) inhibits HepG2 tumor growth in nude mice. Daily HepG2 tumor growth of WAY-600-administrated mice is significantly lower than that of vehicle control mice. Importantly, the in vivo anti-cancer activity by WAY-600 is further potentiated with the co-administration of MEK-162 (2.5 mg/kg, p.o. daily)[2].

    References
    Preparing Stock Solutions
    Concentration Volume Mass 1 mg 5 mg 10 mg
    1 mM 2.0219 mL 10.1094 mL 20.2188 mL
    5 mM 0.4044 mL 2.0219 mL 4.0438 mL
    10 mM 0.2022 mL 1.0109 mL 2.0219 mL
    Please refer to the solubility information to select the appropriate solvent.
    Cell Assay
    [2]

    WAY-600 is prepared in DMSO.

    Established HCC cells (HepG2 and Huh-7), primary HCC cells (Pnt-1/-2/-3/-4), or THLE-2 liver cells are cultured in WAY-600 (1-1000 nM)-containing medium for 24, 48, 72, 96 hours, cell viability is tested by MTT assay[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [2]

    WAY-600 is prepared in 5% ethanol, 5% polysorbate 80, 5% polyethylene glycol-400.

    Mouse: Mice tumor xenografts are randomly divided into four groups (10 mice per group): vehicle ( intraperitoneal or i.p.), WAY-600 (10 mg/kg, i.p. injection), MEK-162 (2.5 mg/kg, oral gavage) or WAY-600 plus MEK-162 combination. The mice are monitored for activity and physical condition on daily basis, and mice body weights and tumor mass are measured weekly[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    494.59

    Formula

    C₂₈H₃₀N₈O

    CAS No.

    1062159-35-6

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    DMSO: 10 mM

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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    Product Name:
    WAY-600
    Cat. No.:
    HY-15272
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