2. Membrane Transporter/Ion Channel
  3. WNK Kinase
  4. WNK463

WNK463 is an orally bioavailable pan-With-No-Lysine (K) (WNK)-kinase inhibitor with IC50s of 5 nM, 1 nM, 6 nM, and 9 nM for WNK1, WNK2, WNK3, and WNK4, respectively.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

CAS No. : 2012607-27-9

사이즈 가격 재고 수량
무료 샘플 (0.1 - 0.2 mg)   지금 신청하기  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 해외재고보유
Solution
10 mM * 1 mL in DMSO 해외재고보유
Solid
5 mg 해외재고보유
10 mg 해외재고보유
25 mg 해외재고보유
50 mg 해외재고보유
100 mg 해외재고보유
200 mg   견적 받기  
500 mg   견적 받기  

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 19 publication(s) in Google Scholar

WNK463 Related Antibodies

Top Publications Citing Use of Products

    WNK463 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 May 27;16(1):4920.  [Abstract]

    WNK463 (0.01, 0.1, 1, 10, 100 μM) produced a dose-dependent inhibition of WNK1 activity as measured by the reduction of phosphorylation of the WNK kinase substrates OXSR1 and STK39.

    WNK463 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 May 27;16(1):4920.  [Abstract]

    WNK463 (1, 3, 10 μM) treatment significantly induced apoptosis in primary AML samples.

    WNK463 purchased from MedChemExpress. Usage Cited in: Sci Signal. 2024 Aug 27;17(851):eade1119.  [Abstract]

    2PK-3 cells pretreated with DMSO, or the indicated concentrations of WNK463 (0.3, 1, 3, 5 μM) or WNK-IN-11 were subjected to a transwell migration assay with CXCL13 (1 μg/mL).

    WNK463 purchased from MedChemExpress. Usage Cited in: Sci Signal. 2024 Aug 27;17(851):eade1119.  [Abstract]

    LifeAct B cells were treated with WNK463 (5 μM) or not and subjected to under agarose migration assay on glass-bottom culture dishes coated with ICAM-1/Fc, CXCL12, and CXCL13.

    WNK463 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2021 Jul 27;12(1):4546.  [Abstract]

    An increase in lactate dehydrogenase (LDH) release in cells treated with ATP, Nigericin, or MSU in the presence of WNK463 (1 μM) compared with controls.

    View All WNK Kinase Isoform Specific Products:

    View All Isoforms
    WNK2 WNK3 WNK4 WNK1

    • Biological Activity

    • 순도&문서

    • References

    • 고객리뷰

    제품 설명

    WNK463 is an orally bioavailable pan-With-No-Lysine (K) (WNK)-kinase inhibitor with IC50s of 5 nM, 1 nM, 6 nM, and 9 nM for WNK1, WNK2, WNK3, and WNK4, respectively[1].

    IC50 & Target

    WNK2

    1 nM (IC50)

    WNK3

    6 nM (IC50)

    WNK4

    9 nM (IC50)

    WNK1

    5 nM (IC50)

    In Vitro

    WNK463 (50 nM, 1 μΜ, 10 μΜ; 6 days; Human tissue-engineered corneas (hTECs)) treatment reduces phosphorylation of the WNK1 downstream targets SPAK/OSR1 in wounded hTECs[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    WNK463 Related Antibodies

    Western Blot Analysis[2]

    Cell Line: Human tissue-engineered corneas (hTECs)
    Concentration: 50 nM, 1 μΜ, 10 μΜ
    Incubation Time: 6 days
    Result: Reduced phosphorylation of the WNK1 downstream targets SPAK/OSR1 in wounded hTECs.
    In Vivo

    WNK463 (1-10 mg/kg; oral administration; 4 hours; Spontaneously hypertensive Sprague Dawley rats) treatment produces dose-dependent decreases in blood pressure and simultaneous increases in heart rate in conscious SHRs. WNK463 produces significant and dose-dependent increases in urine output as well as urinary sodium and potassium excretion rates.
    WNK463 is orally bioavailable in Sprague Dawley rats with a half-life of 2.1 hours[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Spontaneously hypertensive Sprague Dawley rats (34-42 weeks of age) [1]
    Dosage: 1 mg/kg, 3 mg/kg, or 10 mg/kg (Pharmacokinetic study)
    Administration: Oral administration; 4 hours
    Result: Decreased in blood pressure and simultaneous increases in heart rate. WNK463 produced significant and dose-dependent increased in urine output as well as urinary sodium and potassium excretion rates.
    분자량

    463.46

    화학식

    C21H24F3N7O2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    FC(F)(F)C1=NN=C(O1)C2=CN=C(N3CCC(N4C=NC=C4C(NC(C)(C)C)=O)CC3)C=C2
    선적

    Room temperature in continental US; may vary elsewhere.
    보관
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    용액&용해도
    In Vitro: 

    DMSO : ≥ 30 mg/mL (64.73 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1577 mL 10.7884 mL 21.5768 mL
    5 mM 0.4315 mL 2.1577 mL 4.3154 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • 몰농도 계산기

    • 농도 희석 계산기

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    순도&문서

    Purity: 99.75%

    SDS (393 KB)

    COA (251 KB) LCMS (94 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.1577 mL 10.7884 mL 21.5768 mL 53.9421 mL
    5 mM 0.4315 mL 2.1577 mL 4.3154 mL 10.7884 mL
    10 mM 0.2158 mL 1.0788 mL 2.1577 mL 5.3942 mL
    15 mM 0.1438 mL 0.7192 mL 1.4385 mL 3.5961 mL
    20 mM 0.1079 mL 0.5394 mL 1.0788 mL 2.6971 mL
    25 mM 0.0863 mL 0.4315 mL 0.8631 mL 2.1577 mL
    30 mM 0.0719 mL 0.3596 mL 0.7192 mL 1.7981 mL
    40 mM 0.0539 mL 0.2697 mL 0.5394 mL 1.3486 mL
    50 mM 0.0432 mL 0.2158 mL 0.4315 mL 1.0788 mL
    60 mM 0.0360 mL 0.1798 mL 0.3596 mL 0.8990 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    WNK463 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    WNK4632012607-27-9WNK 463WNK-463WNK KinaseWith-no-lysine (WNK) kinaseInhibitorinhibitorinhibit

    최근 본 상품:

    온라인 문의

    Your information is safe with us. * Required Fields.

    상품명

    		  

    Requested Quantity *

    고객명 *

    		 

    호칭

    메일주소 *

    		 

    전화번호 *

    Department

    		 

    회사명 *

    City

    도

    Country or Region *

    		      

    비고

    대량구매 문의

    Inquiry Information

    상품명:
    WNK463
    Cat. No.:
    HY-100626
    수량:
    MCE Japan Authorized Agent:

    고객리뷰

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    상품명

    		  

    Lot #

    고객명 *

    		 

    메일주소 *

    전화번호

    		 

    Department *

    회사명 *

    		 

    Country or Region *

    비고

    SAFETY DATA SHEET (SDS)

    English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB) Italian - IT (393 KB) Korean - KR (393 KB) Portuguese - PT (393 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.