Benzamidine hydrochloride

Name: Benzamidine hydrochloride
Brand: MedChemExpress
SKU: HY-W018781
Rating: 5.0 (3 reviews)

Cat. No.: HY-W018781 Purity: 99.94%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Benzamidine hydrochloride is a competitive protease inhibitor that blocks the hydrolytic cleavage of glucagon by plasmin, trypsin and thrombin. Benzamidine hydrochloride effectively inhibits the degradation of glucagon by relevant proteases during the collection, storage and analysis of human plasma and blood samples. During in vivo metabolism, Benzamidine hydrochloride undergoes N-hydroxylation and produces multiple metabolites, exhibiting characteristics of delayed excretion or biphasic elimination. Benzamidine hydrochloride only induces slight single-strand DNA breaks at high concentrations and shows no significant genotoxic potential overall. Benzamidine hydrochloride may interfere with the detection of some glucagon antisera, but does not affect key antigen-antibody affinity at specific concentrations. Benzamidine hydrochloride can be used as a stabilizer in glucagon radioimmunoassays to ensure the accuracy and recovery rate of detection results.

For research use only. We do not sell to patients.

Benzamidine hydrochloride Chemical Structure

CAS No. : 1670-14-0

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	Get quote
5 g	Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Benzamidine hydrochloride:

Benzamidine In-stock
Benzamidine hydrochloride hydrate In-stock
Benzamidine hydrochloride (Standard) Get quote

3 Publications Citing Use of MCE Benzamidine hydrochloride

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Ki: 20 μM (Tryptase), 21 μM (Trypsin), 97 μM (uPA), 110 μM (Factor Xa), 320 μM (Thrombin), 750 μM (tPA)^[1]

In Vitro

Benzamidine hydrochloride (0.05-0.1 M; 6-24 h at 4°C) inhibits the degradation of [¹²⁵I]-glucagon in undiluted human plasma; the recovery rate of glucagon in undiluted human plasma treated with 0.05 M benzamidine exceeds 85%^[1].
Benzamidine hydrochloride (1-20 μmoles/tube or 5-20 μmoles/tube; 1 h) exhibits only weak, marginal genotoxicity (inducing DNA single-strand breaks) in primary rat hepatocytes, and shows no genotoxicity in CO631 cells at the tested concentrations^[2].
Benzamidine hydrochloride (1-10 μmoles/plate; 72 h) does not induce selective amplification of SV40 DNA in CO631 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Benzamidine (10 mg/kg; intravenous injection; single dose) hydrochloride undergoes N-hydroxylation to form benzamidine oxime in male Wistar rats and grey rabbits. It is also partially excreted via urine, with benzamide detected in the urine^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

156.62

Formula

C₇H₉ClN₂

CAS No.

1670-14-0

Appearance

Solid

Color

White to off-white

SMILES

[H]Cl.N=C(C1=CC=CC=C1)N

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (638.49 mM; Need ultrasonic)

DMSO : 100 mg/mL (638.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.3849 mL	31.9244 mL	63.8488 mL
5 mM	1.2770 mL	6.3849 mL	12.7698 mL
10 mM	0.6385 mL	3.1924 mL	6.3849 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (15.96 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (15.96 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (15.96 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.94%

Select Batch:

Data Sheet (277 KB) SDS (394 KB)

COA (250 KB) HNMR (243 KB) RP-HPLC (264 KB) MS (70 KB)

Handling Instructions (2659 KB)

References

[1]. ENSINCK J W, et al. Use of benzamidine as a proteolytic inhibitor in the radioimmunoassay of glucagon in plasma[J]. The Journal of Clinical Endocrinology & Metabolism, 1972, 35(3): 463-467.

[2]. Clement B, et al. Genotoxic activities of benzamidine and its N-hydroxylated metabolite benzamidoxime in Salmonella typhimurium and mammalian cells. J Cancer Res Clin Oncol. 1988;114(4):363-368. [Content Brief]

[3]. Clement B, et al. Biotransformation of benzamidine and benzamidoxime in vivo. Arch Pharm (Weinheim). 1993;326(10):807-812. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	6.3849 mL	31.9244 mL	63.8488 mL	159.6220 mL
	5 mM	1.2770 mL	6.3849 mL	12.7698 mL	31.9244 mL
	10 mM	0.6385 mL	3.1924 mL	6.3849 mL	15.9622 mL
	15 mM	0.4257 mL	2.1283 mL	4.2566 mL	10.6415 mL
	20 mM	0.3192 mL	1.5962 mL	3.1924 mL	7.9811 mL
	25 mM	0.2554 mL	1.2770 mL	2.5540 mL	6.3849 mL
	30 mM	0.2128 mL	1.0641 mL	2.1283 mL	5.3207 mL
	40 mM	0.1596 mL	0.7981 mL	1.5962 mL	3.9906 mL
	50 mM	0.1277 mL	0.6385 mL	1.2770 mL	3.1924 mL
	60 mM	0.1064 mL	0.5321 mL	1.0641 mL	2.6604 mL
	80 mM	0.0798 mL	0.3991 mL	0.7981 mL	1.9953 mL
	100 mM	0.0638 mL	0.3192 mL	0.6385 mL	1.5962 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.