2. Metabolic Enzyme/Protease
  3. Ser/Thr Protease
  4. WNK1-IN-1

WNK1-IN-1 is a selective inhibitor of WNK1 with an IC50 value of 1.6 μM. WNK1-IN-1 inhibits OSR1 phosphorylation with an IC50 value of 4.3 μM. WNK1-IN-1 can be used for the research of blood pressure regulation and cancer.

For research use only. We do not sell to patients.

WNK1-IN-1 Chemical Structure

WNK1-IN-1 Chemical Structure

CAS No. : 324022-39-1

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10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

WNK1-IN-1 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

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Description

WNK1-IN-1 is a selective inhibitor of WNK1 with an IC50 value of 1.6 μM. WNK1-IN-1 inhibits OSR1 phosphorylation with an IC50 value of 4.3 μM. WNK1-IN-1 can be used for the research of blood pressure regulation and cancer[1].

IC50 & Target

IC50: 1.6 μM (WNK1), 4.3 μM (OSR1 phosphorylation)[1]
In Vitro

WNK1-IN-1 (0-1000 μM; 30 min) shows high potency to WNK1 with an IC50 value of 1.6 μM[1].
WNK1-IN-1 (0.2-12.5 μM; 24 h) inhibits endogenous OSR1 phosphorylation with an IC50 value of 4.3 μM in MDAMB231 breast-cancer cells[1].
WNK1-IN-1 (0-100 mM) is 10-fold more potent to WNK1 than WNK3 in Kinase-Glo assay[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

WNK1-IN-1 Related Antibodies
Molecular Weight

446.14

Formula

C13H15BrCl2N2O4S
CAS No.
Appearance

Solid

Color

Light yellow to green yellow

SMILES

O=[N+](C1=CC(S(=O)(C(Cl)(Br)Cl)=O)=CC=C1NC2CCCCC2)[O-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (224.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2414 mL 11.2072 mL 22.4145 mL
5 mM 0.4483 mL 2.2414 mL 4.4829 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (5.60 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (5.60 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2414 mL 11.2072 mL 22.4145 mL 56.0362 mL
5 mM 0.4483 mL 2.2414 mL 4.4829 mL 11.2072 mL
10 mM 0.2241 mL 1.1207 mL 2.2414 mL 5.6036 mL
15 mM 0.1494 mL 0.7471 mL 1.4943 mL 3.7357 mL
20 mM 0.1121 mL 0.5604 mL 1.1207 mL 2.8018 mL
25 mM 0.0897 mL 0.4483 mL 0.8966 mL 2.2414 mL
30 mM 0.0747 mL 0.3736 mL 0.7471 mL 1.8679 mL
40 mM 0.0560 mL 0.2802 mL 0.5604 mL 1.4009 mL
50 mM 0.0448 mL 0.2241 mL 0.4483 mL 1.1207 mL
60 mM 0.0374 mL 0.1868 mL 0.3736 mL 0.9339 mL
80 mM 0.0280 mL 0.1401 mL 0.2802 mL 0.7005 mL
100 mM 0.0224 mL 0.1121 mL 0.2241 mL 0.5604 mL
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WNK1-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

WNK1-IN-1324022-39-1Ser/Thr ProteaseSerine proteasesSerine endopeptidasesThreonine proteasesTrihalo-sulfonesmall-moleculehalogen bondATP binding pocketOSR1MDAMB231phosphorylationInhibitorinhibitorinhibit

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