2. Cell Cycle/DNA Damage Immunology/Inflammation Apoptosis
  3. DNA/RNA Synthesis IFNAR Apoptosis Necroptosis
  4. ADAR1i-124

ADAR1i-124 is an A-to-I RNA editing inhibitor by inhibiting the catalytic activities of both ADAR1p150 and ADAR1p110. ADAR1i-124 activates type I interferon (IFN) and ZBP1 pathways and dose-dependently inhibits viability across different types of cancer cell lines. ADAR1i-124 can induce cells apoptosis and necroptosis. ADAR1i-124 can be used for the research of cancer, such as cutaneous melanoma and ovarian cancer.

For research use only. We do not sell to patients.

ADAR1i-124

ADAR1i-124 Chemical Structure

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg   Get quote  
200 mg   Get quote  

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ADAR1i-124 Related Antibodies

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Description

ADAR1i-124 is an A-to-I RNA editing inhibitor by inhibiting the catalytic activities of both ADAR1p150 and ADAR1p110. ADAR1i-124 activates type I interferon (IFN) and ZBP1 pathways and dose-dependently inhibits viability across different types of cancer cell lines. ADAR1i-124 can induce cells apoptosis and necroptosis. ADAR1i-124 can be used for the research of cancer, such as cutaneous melanoma and ovarian cancer[1].

In Vitro

ADAR1i-124 (0-200 μM; 2 h) potently inhibits ADAR1p150-mediated A-to-I RNA editing in vitro with IC50 values of 8.8 μM (position 2) and 6.5 μM (position 5)[1].
ADAR1i-124 (300 μM; 2 h) does not inhibit ADAR2-mediated A-to-I RNA editing in vitro[1].
ADAR1i-124 binds selectively to ADAR1p110 with higher affinity than ADAR2 and does not bind to GAPDH[1].
ADAR1i-124 (10 μM; 12 h) induces unedited dsRNA located with MDA5 within the filament structures in mouse Yumm1.7 cutaneous melanoma cells[1].
ADAR1i-124 (10 μM; 12 h) decreases A-to-I editing and prompts the transition of certain dsRNAs from A-form to Z-form in Yumm1.7 cells[1].
ADAR1i-124 (48 h) induces dose-dependent cytotoxicity against mouse (Yumm1.7 CM, HSG2 OC) and human (WM4223 AM, WM3000 CM) cancer cells with IC50 values of 2.3, 1.2, 1.1 and 6.6 μM[1].
ADAR1i-124 (10 μM; 24 h) activates IFN signaling in Yumm1.7 melanoma and HGS2 OC cells[1].
ADAR1i-124 (10 μM; 24 h) induces apoptosis in HeLa and Yumm1.7 cells and also induces necroptosis activated by the ZBP1 pathway in Yumm1.7 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ADAR1i-124 Related Antibodies

Real Time qPCR[1]

Cell Line: Yumm1.7 melanoma and HGS2 OC cells
Concentration: 10 μM
Incubation Time: 24 h
Result: Upre-gulated expression of IFN-stimulated genes such as Ifih1 (MDA5), Cxcl9, and Cxcl10.
Molecular Weight

325.75

Formula

C17H12ClN3O2
Appearance

Solid

Color

White to off-white

SMILES

OCN1C(C(NC2=CC=C(C#N)C=C2)=O)=C(Cl)C3=C1C=CC=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (306.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0698 mL 15.3492 mL 30.6984 mL
5 mM 0.6140 mL 3.0698 mL 6.1397 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.67 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (7.67 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.71%

SDS (252 KB)

COA (251 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0698 mL 15.3492 mL 30.6984 mL 76.7460 mL
5 mM 0.6140 mL 3.0698 mL 6.1397 mL 15.3492 mL
10 mM 0.3070 mL 1.5349 mL 3.0698 mL 7.6746 mL
15 mM 0.2047 mL 1.0233 mL 2.0466 mL 5.1164 mL
20 mM 0.1535 mL 0.7675 mL 1.5349 mL 3.8373 mL
25 mM 0.1228 mL 0.6140 mL 1.2279 mL 3.0698 mL
30 mM 0.1023 mL 0.5116 mL 1.0233 mL 2.5582 mL
40 mM 0.0767 mL 0.3837 mL 0.7675 mL 1.9186 mL
50 mM 0.0614 mL 0.3070 mL 0.6140 mL 1.5349 mL
60 mM 0.0512 mL 0.2558 mL 0.5116 mL 1.2791 mL
80 mM 0.0384 mL 0.1919 mL 0.3837 mL 0.9593 mL
100 mM 0.0307 mL 0.1535 mL 0.3070 mL 0.7675 mL
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ADAR1i-124 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ADAR1i-124DNA/RNA SynthesisIFNARApoptosisNecroptosisInterferon-α/β receptorInterferon-alpha/beta receptor A-to-I RNA editinginterferon signalingdsRNAMDA5ADARcutaneous melanomaADAR1p150ADAR1p110HeLaYumm1.7Inhibitorinhibitorinhibit

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