Ametantrone (Synonyms: NSC 196473; NSC 290813)

Cat. No.: HY-13550 Purity: 97.28%: Data Sheet
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Ametantrone (NSC 196473) is an antitumor agent that intercalates into DNA and induces topoisomerase II (TOP2)-mediated DNA break.

For research use only. We do not sell to patients.

Ametantrone Chemical Structure

CAS No. : 64862-96-0

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
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50 mg	Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Ametantrone (NSC 196473) is an antitumor agent that intercalates into DNA and induces topoisomerase II (TOP2)-mediated DNA break^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	1108 nM Compound: Ametantrone	In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line A2780/Dx(RI) In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line A2780/Dx(RI)	[PMID: 10479282]
A2780	IC₅₀	180 nM Compound: Ametantrone	In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line A2780 In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line A2780	[PMID: 10479282]
DU-145	IC₅₀	1020 nM Compound: Ametantrone	In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line DU145 In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line DU145	[PMID: 10479282]
HL-60	ED₅₀	0.88 mM Compound: AM	Effective concentration against cell growth of HL60 cell line by 50% Effective concentration against cell growth of HL60 cell line by 50%	[PMID: 8759632]
HL-60	GI₅₀	29 nM Compound: AT, ametantrone	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	[PMID: 17962028]
HL-60	IC₅₀	15.9 nM Compound: Ametantrone	Compound was tested in vitro for cytotoxicity against HL60 human leukemia cell line (72 hr exposure to compound) Compound was tested in vitro for cytotoxicity against HL60 human leukemia cell line (72 hr exposure to compound)	[PMID: 10479282]
HL60/MX2	GI₅₀	430 nM Compound: AT, ametantrone	Cytotoxicity against DNA topoisomerase-2 deficient human HL60/MX2 cells by MTT assay Cytotoxicity against DNA topoisomerase-2 deficient human HL60/MX2 cells by MTT assay	[PMID: 17962028]
HeLa	EC₅₀	0.17 μg/mL Compound: 2	Effective concentration required to inhibit by 50% the growth of HeLa S3 cells Effective concentration required to inhibit by 50% the growth of HeLa S3 cells	[PMID: 3172129]
HeLa	ED₅₀	3.1 mM Compound: AM	Effective concentration against cell growth of HeLa cell line by 50% Effective concentration against cell growth of HeLa cell line by 50%	[PMID: 8759632]
HeLa	IC₅₀	0.17 μg/mL Compound: ametantrone	In vitro cytotoxicity against HeLa S3 cell lines In vitro cytotoxicity against HeLa S3 cell lines	[PMID: 2213837]
HeLa	IC₅₀	0.9 μM Compound: Ametantrone	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 27521587]
HeLa	IC₅₀	0.9 μM Compound: Ametantrone	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 28342691]
K562	IC₅₀	12392 nM Compound: Ametantrone	Compound was tested in vitro for cytotoxicity against MDR cell line K562R (RI) (72 hr exposure to compound) Compound was tested in vitro for cytotoxicity against MDR cell line K562R (RI) (72 hr exposure to compound)	[PMID: 10479282]
K562	IC₅₀	181 nM Compound: Ametantrone	Compound was tested in vitro for cytotoxicity against MDR cell line K562R (72 hr exposure to compound) Compound was tested in vitro for cytotoxicity against MDR cell line K562R (72 hr exposure to compound)	[PMID: 10479282]
L1210	EC₅₀	0.21 g/mL Compound: ametantrone	Effective concentration against inhibition of L1210 mouse leukemia cells in tissue culture Effective concentration against inhibition of L1210 mouse leukemia cells in tissue culture	[PMID: 1995874]
L1210	EC₅₀	1.77 μg/mL Compound: 2	Effective concentration required to inhibit by 50% the growth of L1210 cells Effective concentration required to inhibit by 50% the growth of L1210 cells	[PMID: 3172129]
L1210	EC₅₀	275 nM Compound: AME	Concentration of compound required to inhibit the cellular protein biosynthesis by 50% Concentration of compound required to inhibit the cellular protein biosynthesis by 50%	[PMID: 8421288]
L1210	ED₅₀	0.1 mM Compound: AM	Effective concentration against cell growth of L1210 cell line by 50% Effective concentration against cell growth of L1210 cell line by 50%	[PMID: 8759632]
L1210	ED₅₀	2.54 μg/mL Compound: 1 (ametantrone)	Concentration required to inhibit the growth of L1210 leukemia cells Concentration required to inhibit the growth of L1210 leukemia cells	[PMID: 2754697]
L1210	IC₅₀	0.03 μg/mL Compound: 1a (Ametantrone)	Tested in vitro for inhibitory activity against L1210 murine leukemia Tested in vitro for inhibitory activity against L1210 murine leukemia	[PMID: 8145234]
L1210	IC₅₀	1.77 μg/mL Compound: ametantrone	In vitro inhibitory activity against murine L1210 leukemia In vitro inhibitory activity against murine L1210 leukemia	[PMID: 1732555]
L1210	IC₅₀	146 nM Compound: Ametantrone	Compound was tested in vitro for cytotoxicity against L1210 murine cell line (48 hr exposure to compound) Compound was tested in vitro for cytotoxicity against L1210 murine cell line (48 hr exposure to compound)	[PMID: 10479282]
L1210	IC₅₀	3.65 μM Compound: ametantrone	In vitro inhibitory activity against murine L1210 leukemia In vitro inhibitory activity against murine L1210 leukemia	[PMID: 1732555]
LoVo	IC₅₀	0.4 μg/mL Compound: 1a (Ametantrone)	Tested in vitro for cytotoxic activity against Human colon Adenocarcinoma sensitive cell line (LoVo) Tested in vitro for cytotoxic activity against Human colon Adenocarcinoma sensitive cell line (LoVo)	[PMID: 8145234]
LoVo	IC₅₀	34.7 μg/mL Compound: 1a (Ametantrone)	Tested in vitro for cytotoxic activity against Doxorubicin resistant Human colon Adenocarcinoma sensitive cell line Tested in vitro for cytotoxic activity against Doxorubicin resistant Human colon Adenocarcinoma sensitive cell line	[PMID: 8145234]
LoVo	IC₅₀	36802 nM Compound: Ametantrone	In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line LoVo/DX(RI) In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line LoVo/DX(RI)	[PMID: 10479282]
LoVo	IC₅₀	641 nM Compound: Ametantrone	In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line LoVo In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line LoVo	[PMID: 10479282]
LoVo	IC₅₀	5.8 x 10^-7 M Compound: Ametantrone	Inhibitory activity against human colon adenocarcinoma cell line LoVo. Inhibitory activity against human colon adenocarcinoma cell line LoVo.	[PMID: 7853345]
LoVo	IC₅₀	9.2 x 10^-5 M Compound: Ametantrone	Inhibitory activity against human colon adenocarcinoma cell line LoVo/Dx. Inhibitory activity against human colon adenocarcinoma cell line LoVo/Dx.	[PMID: 7853345]
MCF7	GI₅₀	4.97 μM Compound: AT, ametantrone	Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay	[PMID: 17962028]
MCF7	IC₅₀	0.51 μM Compound: Ametantrone	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 27521587]
MCF7	IC₅₀	0.51 μM Compound: Ametantrone	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 28342691]
NCI-H460	GI₅₀	2.55 μM Compound: AT, ametantrone	Cytotoxicity against human NCI-H460 cells after 48 hrs by SRB assay Cytotoxicity against human NCI-H460 cells after 48 hrs by SRB assay	[PMID: 17962028]
PC-3	GI₅₀	8.52 μM Compound: AT, ametantrone	Cytotoxicity against human PC3 cells after 48 hrs by SRB assay Cytotoxicity against human PC3 cells after 48 hrs by SRB assay	[PMID: 17962028]
PC-3	IC₅₀	1848 nM Compound: Ametantrone	In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line PC3 In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line PC3	[PMID: 10479282]
SF-268	GI₅₀	2.45 μM Compound: AT, ametantrone	Cytotoxicity against human SF268 cells after 48 hrs by SRB assay Cytotoxicity against human SF268 cells after 48 hrs by SRB assay	[PMID: 17962028]

Molecular Weight

412.48

Formula

C₂₂H₂₈N₄O₄

CAS No.

64862-96-0

Appearance

Solid

Color

Brown to black

SMILES

O=C1C2=C(C=CC=C2)C(C3=C(NCCNCCO)C=CC(NCCNCCO)=C13)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 1.67 mg/mL (4.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4243 mL	12.1217 mL	24.2435 mL
5 mM	---	---	---
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 97.28%

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Data Sheet (266 KB) SDS (393 KB)

COA (246 KB) HNMR (286 KB) LCMS (90 KB)

Handling Instructions (2659 KB)

References

[1]. Lee CH, et al. Anthracenedione-methionine conjugates are novel topoisomerase II-targeting anticancer agents with favorable drug resistance profiles. Biochem Pharmacol. 2012;83(9):1208-1216. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO

1 mM

2.4243 mL

12.1217 mL

24.2435 mL

60.6087 mL

Ametantrone Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ametantrone64862-96-0NSC 196473 NSC 290813NSC196473NSC-196473NSC290813NSC 290813NSC-290813DNA/RNA SynthesisDNA breakantitumor agentanthracenedioneDNA bindingInhibitorinhibitorinhibit

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Ametantrone (Synonyms: NSC 196473; NSC 290813)

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Ametantrone Related Antibodies

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Biological Activity

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References

Customer Review

Molarity Calculator

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Complete Stock Solution Preparation Table

Ametantrone Related Classifications

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