2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. NO Synthase Reactive Oxygen Species (ROS)
  4. BN80933

BN80933 is a selective neuronal nitric oxide synthase (nNOS) inhibitor with a rat Ki of 0.92 μM. BN80933 inhibits lipid peroxidation, and blocks hypoxia-induced lactate dehydrogenase elevation and delayed 8-epiprostaglandin F2α elevation. BN80933 can be used for the research of stroke, and traumatic brain injury.

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BN80933

BN80933 Chemical Structure

CAS No. : 214348-10-4

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Description

BN80933 is a selective neuronal nitric oxide synthase (nNOS) inhibitor with a rat Ki of 0.92 μM. BN80933 inhibits lipid peroxidation, and blocks hypoxia-induced lactate dehydrogenase elevation and delayed 8-epiprostaglandin F2α elevation. BN80933 can be used for the research of stroke, and traumatic brain injury[1][2][3].

IC50 & Target[1]

nNOS

 

In Vitro

BN80933 (0.01-3 μM; 24 h) potently protects primary rat cortical neuron-glia cultures from hypoxia-induced cell death with an IC50 of 0.15 μM[1].
BN80933 (24 h) potently protects mouse hippocampal HT-22 cells from BSO (HY-106376)-induced oxidative cell death with an IC50 of 0.06 μM[1].
BN80933 (24 h) potently protects mouse hippocampal HT-22 cells from Glutamate-induced oxidative cell death with an IC50 of 0.13 μM, and maintains protective efficacy when administered 6 h after glutamate exposure[1].
BN80933 potently inhibits iron-dependent lipid peroxidation in rat cerebral cortex membrane homogenate with an IC50 of 0.31 μM, a potency that correlates strongly with its neuroprotective activity[1].
BN80933 (3 μM; 24 h) prevents glutamate-induced 8-isoprostane production but does not reverse GSH depletion in mouse hippocampal HT-22 cells, indicating it acts downstream of GSH loss to inhibit oxidative lipid peroxidation[1].
BN80933 (15 min) potently and competitively inhibits rat cerebellum nNOS with a Ki of 0.92 μM[2].
BN80933 (45 min) potently inhibits iron-dependent lipid peroxidation in rat cerebral cortex membrane homogenate with an IC50 of 0.29 μM[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BN80933 Related Antibodies

Cell Cytotoxicity Assay[1]

Cell Line: primary rat cortical neuron-glia cultures (12-14 days in vitro)
Concentration: 0.01 μM; 0.03 μM; 0.1 μM; 0.3 μM; 1 μM; 3 μM
Incubation Time: 24 h
Result: Completely prevented hypoxia-induced morphological neuronal injury and elevation of LDH activity at 3 μM.
Exhibited concentration-dependent effect on LDH release in the submicromolar range, with near-maximal protection at 0.3 μM and an IC50 value of 0.15 μM.

ELISA Assay[1]

Cell Line: mouse hippocampal HT-22 cells
Concentration: 3 μM
Incubation Time: 6 h (GSH measurement); 24 h (8-isoprostane measurement)
Result: Did not modify the glutamate-induced depletion of intracellular GSH levels measured 6 h after exposure.
Reduced glutamate-induced elevation of 8-isoprostane levels (from 807 pg/mL to 296 pg/mL) measured 24 h after exposure.
Did not affect GSH or 8-isoprostane levels in untreated control cells.
In Vivo

BN80933 (3-10 mg/kg; i.v.; single dose) provides significant neuroprotection in mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Swiss mice (male, 22 to 25 g, transient focal cerebral ischemia induced by 15-minute left middle cerebral artery occlusion via intraluminal filament technique followed by reperfusion)[3]
Dosage: 3 mg/kg; 10 mg/kg
Administration: i.v.; single dose
Result: Improved grip score to 26.8 s, reduced infarct volume by 31% to 62 mm3, reduced Evans blue extravasation by 75% to 1.7 μg/g tissue, reduced brain water content by 37% to 83.8%, and reduced myeloperoxidase activity by 56% to 0.064 U/g tissue at 24 hours post-ischemia (3 mg/kg).
Improved grip score to 23.1 s, reduced infarct volume by 36% to 58 mm3, reduced Evans blue extravasation by 42% to 4.0 μg/g tissue, and reduced myeloperoxidase activity by 45% to 0.078 U/g tissue at 24 hours post-ischemia (10 mg/kg).
Improved grip score to 21.9 s and reduced infarct volume by 31% to 51 mm3 at 48 hours post-ischemia (3 mg/kg).
Improved grip score to 22.4 s and reduced infarct volume by 26% to 55 mm3 at 48 hours post-ischemia (10 mg/kg).
Molecular Weight

518.67

Formula

C29H34N4O3S
CAS No.
SMILES

CC1=C2C(O[C@@](C)(CC2)C(N3CCN(C4=CC=C(C=C4)NC(C5=CC=CS5)=N)CC3)=O)=C(C(C)=C1O)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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BN80933 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BN80933214348-10-4BN 80933BN-80933NO SynthaseReactive Oxygen Species (ROS)Nitric oxide synthasesNOSneuronal nitric oxide synthasehypoxiaratmouse hippocampal HT-22 cellsmiddle cerebral artery occlusioncerebral cortex membrane homogenateprimary rat cortical neuron-glia culturesbuthionine sulfoximineglutamatelipid peroxidationInhibitorinhibitorinhibit

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