Thionin acetate
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Thionin acetate (Thionine acetate) is a compound present in the seeds, stems, roots, and leaves of many plant species with antibacterial activity. Thionin acetate is a metachromatic cationic histological dye widely used in biological staining.
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- Pureté: 93.00%
- CAS No.: 78338-22-4
- Formule: C14H13N3O2S
- Masse moléculaire:287.34
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Stockage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Huh-7 | CC50 |
4.67 μg/mL
Compound: 20
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Cytotoxic concentration of compound HC40 cells determined by using MTT assay
Cytotoxic concentration of compound HC40 cells determined by using MTT assay
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[PMID: 10866369] |
| Huh-7 | IC50 |
2.86 μg/mL
Compound: 20
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Anti-Hepatitis C Viral IRES activity of compound by measured as Fluci signal in HC40 cells
Anti-Hepatitis C Viral IRES activity of compound by measured as Fluci signal in HC40 cells
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[PMID: 10866369] |
Thionin acetate can be used for tissue staining, such as staining brainstem slices. Thionin acetate can assist in observing and analyzing the brainstem structure, determining the location of the preB?tC and the characteristics of related regions[2].
The brief steps of Thionin acetate staining are as follows: First, the tissue sections are sequentially treated with 70% ethanol, 50% ethanol, and distilled water, then immersed in the Thionin acetate solution. Subsequently, they are washed successively with distilled water and ethanol of different concentrations, and finally cleared in xylene to complete the staining for observation and analysis[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 78338-22-4
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Appearance Solid
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Masse moléculaire 287.34
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Formule C14H13N3O2S
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Color Green to dark green
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SMILES
NC1=CC2=[S+]C3=C(C=CC(N)=C3)N=C2C=C1.CC([O-])=O
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Synonyms
Thionine acetate
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvant et solubilité
DMSO : 41.67 mg/mL (145.02 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Florack DE, et al. Thionins: properties, possible biological roles and mechanisms of action. Plant Mol Biol. 1994 Oct;26(1):25-37. [Content Brief]
[2]. Ruangkittisakul A, et al. Identification of the pre-Bötzinger complex inspiratory center in calibrated "sandwich" slices from newborn mice with fluorescent Dbx1 interneurons. Physiol Rep. 2014 Aug 19;2(8):e12111. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4802 mL | 17.4010 mL | 34.8020 mL | 87.0049 mL |
| 5 mM | 0.6960 mL | 3.4802 mL | 6.9604 mL | 17.4010 mL | |
| 10 mM | 0.3480 mL | 1.7401 mL | 3.4802 mL | 8.7005 mL | |
| 15 mM | 0.2320 mL | 1.1601 mL | 2.3201 mL | 5.8003 mL | |
| 20 mM | 0.1740 mL | 0.8700 mL | 1.7401 mL | 4.3502 mL | |
| 25 mM | 0.1392 mL | 0.6960 mL | 1.3921 mL | 3.4802 mL | |
| 30 mM | 0.1160 mL | 0.5800 mL | 1.1601 mL | 2.9002 mL | |
| 40 mM | 0.0870 mL | 0.4350 mL | 0.8700 mL | 2.1751 mL | |
| 50 mM | 0.0696 mL | 0.3480 mL | 0.6960 mL | 1.7401 mL | |
| 60 mM | 0.0580 mL | 0.2900 mL | 0.5800 mL | 1.4501 mL | |
| 80 mM | 0.0435 mL | 0.2175 mL | 0.4350 mL | 1.0876 mL | |
| 100 mM | 0.0348 mL | 0.1740 mL | 0.3480 mL | 0.8700 mL |
Thionin acetate Related Classifications
HY-D0955 Related Classifications
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