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Cat. No.: HY-W612175

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H-Ala-pNA

H-Ala-pNA is an L-amino acid p-nitroaniline (pNA) derivative and a specific substrate for leukotriene A4 hydrolase. The D-enantiomer of H-Ala-pNA shows no activity toward leukotriene A4 hydrolase. H-Ala-pNA can be catalytically hydrolyzed by leukotriene A4 hydrolase, and the p-nitroaniline produced during the reaction is monitored spectrophotometrically at 405 nm to enable quantitative detection of enzyme activity. H-Ala-pNA is used to evaluate the potency of inhibitors targeting the amidase activity of leukotriene A4 hydrolase.

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CAS No.: 1668-13-9
Formule: C₉H₁₁N₃O₃
Masse moléculaire:209.20

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Please store the product under the recommended conditions in the Certificate of Analysis.

H-Ala-pNA Chemical Structure

CAS No.: 1668-13-9

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Activité biologique

Description

Voir tous les produits spécifiques à Isoform Leukotriene Receptor :

In Vitro

H-Ala-pNA (1.5 mM) is hydrolyzed by purified human leukocyte leukotriene A₄ hydrolase with a K_m of 500 μM, kcat of 6.0×10^-1 s^-1, Vmax of 530 nmol/min per mg, and kcat/K_m of 1.2×10³ M^-1s^-1^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 1668-13-9
Masse moléculaire 209.20
Formule C₉H₁₁N₃O₃
SMILES

C[C@H](N)C(NC1=CC=C(C=C1)N(=O)=O)=O
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureté et documentation

Instruction de manipulation (2659 KB)

Références

[1]. Yuan W, et al. Development of selective tight-binding inhibitors of leukotriene A4 hydrolase. J Med Chem. 1993;36(2):211-220. [Content Brief]

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How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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H-Ala-pNA

Voir tous les produits spécifiques à Isoform Leukotriene Receptor

Activité biologique

Chemical Information

Pureté et documentation

Références

Calculators

Help & FAQs

H-Ala-pNA Related Classifications

HY-W612175 Related Classifications