Carbenoxolone disodium

Name: Carbenoxolone disodium
Brand: MedChemExpress
SKU: HY-B1367
Rating: 5.0 (10 reviews)

Cat. No.: HY-B1367 Purity: 99.94%: Data Sheet
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Carbenoxolone disodium is the active metabolite of Glycyrrhizic acid (HY-N0184) and the inhibitor of human 11β-HSD and bacterial 3α, 20β-HSD. Carbenoxolone disodium is an uncoupling agent for gap junctions and a potent inhibitor of Vaccinia virus replication. Carbenoxolone disodium is used for the study of peptic, esophageal and oral ulceration and inflammation. Carbenoxolone disodium inhibits Vaccinia virus replication.

For research use only. We do not sell to patients.

CAS No. : 7421-40-1

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 10 publication(s) in Google Scholar

Other Forms of Carbenoxolone disodium:

Carbenoxolone Get quote

10 Publications Citing Use of MCE Carbenoxolone disodium

Bio/Physico-chemical Assay

Cell Imaging/Staining

: Carbenoxolone disodium purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Feb 1:227:64-79. [Abstract]; Western blot analysis of p-FOXO3a (S253) and FOXO3a protein in BV2 cells post-Carbenoxolone disodium (CBX; 100 μM) and H₂O₂/zinc treatment.

: Carbenoxolone disodium purchased from MedChemExpress. Usage Cited in: Glia. 2025 Apr 4. [Abstract]; Evaluation of GJIC in different cell groups by assessing thespread of fluorescent dye LY in cultured astrocyte syncytia via scrape-loading. The results showed that LYdiffusion was significantly inhibited in cells treated with Carbenoxolone disodium (CBX; 50 μM), similar to the effects of CXCL12 and CXCL11 .

: Carbenoxolone disodium purchased from MedChemExpress. Usage Cited in: Nat Neurosci. 2024 Aug;27(8):1522-1533. [Abstract]; The gap-junction dependency of FLA-evoked astrocytic Ca2+ wave and ATP bursts (n = 10/9/7 slices from 4/4/3 mice for ACSF, 1-Octanol and Carbenoxolone disodium; CBX; 100 µM; 5 min).

: Carbenoxolone disodium purchased from MedChemExpress. Usage Cited in: Transl Res. 2024 May 9:271:26-39. [Abstract]; HK-2 cells were treated with PANX1 inhibitor PBN (1 mM),and Carbenoxolone disodium (CBX; 100 μM) with or without ADR. Intracellular lipid content was detected by oil red O staining.

: Carbenoxolone disodium purchased from MedChemExpress. Usage Cited in: Transl Res. 2024 May 9:271:26-39. [Abstract]; HK-2 cells were treated with PANX1 inhibitor PBN (1 mM), and Carbenoxolone disodium (CBX; 100 μM) with or without ADR. The protein expressions of Fibronectin, PANX1, PPAR α, and PANK1 were analyzed by western blotting.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Carbenoxolone disodium is the active metabolite of Glycyrrhizic acid (HY-N0184) and the inhibitor of human 11β-HSD and bacterial 3α, 20β-HSD^[1]. Carbenoxolone disodium is an uncoupling agent for gap junctions and a potent inhibitor of Vaccinia virus replication^[2]. Carbenoxolone disodium is used for the study of peptic, esophageal and oral ulceration and inflammation. Carbenoxolone disodium inhibits Vaccinia virus replication.

IC₅₀ & Target

IC50: human 11β-HSD; bacterial 3α, 20β-HSD^[1]; gap junction; Vaccinia virus^[2]

In Vitro

Carbenoxolone disodium (6-150 μM; pre-treatment 1 hour) inhibits Vaccinia virus (VACV) replication in a gap junction-independent in HaCaT cells, and it has toxicity effects on VACV-A5L-EGFP infected cells at 48 h^[2].
Carbenoxolone (30 μM; pre-treatment 1 hour) does not upregulate PP2A expression, but induces the late protein A27 expression in hacat cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	HaCaT cells
Concentration:	6 μM, 12 μM, 30 μM, 60 μM, 150 μM
Incubation Time:	Pre-treatment 1 hour
Result:	Had no toxicity until 48 hours at high dose in virus-infected cells.

Western Blot Analysis^[2]

Cell Line:	HaCaT cells
Concentration:	30 μM
Incubation Time:	Pre-treatment 1 hour
Result:	Presented an obvious upregulation of A27.

In Vivo

Carbenoxolone (intraperitoneal injection; 100, 200 and 300 mg/kg; 30, 60 and 60 min before Diazepam) does not induce a muscle relaxant activity and shows muscle relaxant activity compared to normal saline, and this effect was more than diazepam in the traction test^[3].
Carbenoxolone (intraperitoneal injection; 100, 200 and 300 mg/kg; 30, 60 and 60 min before Pentylenetetrazole) significantly increases sleeping time and decreases latency in mice as a dose-dependent manner in Pentylenetetrazole (PTZ) Seizure model. The ED₅₀ value is 83.3 mg/kg (%95 CL:556.29)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male BALB/c mice^[3]
Dosage:	100, 200 and 300 mg/kg
Administration:	Intraperitoneal injection; 30, 60 and 60 min before Pentylenetetrazole
Result:	Significantly increased the sleeping time in mice.

Molecular Weight

614.72

Formula

C₃₄H₄₈Na₂O₇

CAS No.

7421-40-1

Appearance

Solid

Color

White to off-white

SMILES

CC(C)([C@]1([H])CC[C@@]([C@@]2(CC[C@]3(CC[C@](C[C@@]3([H])C2=C4)(C)C(O[Na])=O)C)C)5C)[C@@H](OC(CCC(O[Na])=O)=O)CC[C@]1(C)[C@@]5([H])C4=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (81.34 mM; Need ultrasonic)

DMSO : 11.67 mg/mL (18.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6268 mL	8.1338 mL	16.2676 mL
5 mM	0.3254 mL	1.6268 mL	3.2535 mL
10 mM	0.1627 mL	0.8134 mL	1.6268 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.03 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (2.03 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (2.03 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 6.25 mg/mL (10.17 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.94%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (250 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. W L Duax, et al. Steroid Dehydrogenase Structures, Mechanism of Action, and Disease. Vitam Horm. 2000;58:121-48. [Content Brief]

[2]. Hossein Hosseinzadeh, et al. Anticonvulsant, Sedative and Muscle Relaxant Effects of Carbenoxolone in Mice. BMC Pharmacol. 2003 Apr 29;3:3. [Content Brief]

[3]. Ismar R Haga, et al. Carbenoxolone-mediated Cytotoxicity Inhibits Vaccinia Virus Replication in a Human Keratinocyte Cell Line. Sci Rep. 2018 Nov 16;8(1):16956. [Content Brief]

[4]. Ismar R Haga, et al. Carbenoxolone-mediated cytotoxicity inhibits Vaccinia virus replication in a human keratinocyte cell line. Sci Rep. 2018 Nov 16;8(1):16956. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	1.6268 mL	8.1338 mL	16.2676 mL	40.6689 mL
	5 mM	0.3254 mL	1.6268 mL	3.2535 mL	8.1338 mL
	10 mM	0.1627 mL	0.8134 mL	1.6268 mL	4.0669 mL
	15 mM	0.1085 mL	0.5423 mL	1.0845 mL	2.7113 mL
H₂O	20 mM	0.0813 mL	0.4067 mL	0.8134 mL	2.0334 mL
	25 mM	0.0651 mL	0.3254 mL	0.6507 mL	1.6268 mL
	30 mM	0.0542 mL	0.2711 mL	0.5423 mL	1.3556 mL
	40 mM	0.0407 mL	0.2033 mL	0.4067 mL	1.0167 mL
	50 mM	0.0325 mL	0.1627 mL	0.3254 mL	0.8134 mL
	60 mM	0.0271 mL	0.1356 mL	0.2711 mL	0.6778 mL
	80 mM	0.0203 mL	0.1017 mL	0.2033 mL	0.5084 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.