Art. -Nr.: HY-N10790

RA-V

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RA-V is a cyclic hexapeptide. RA-V has activity against Wnt, Myc and Notch with IC₅₀ values of 50, 75, and 93 ng/mL, respectively. RA-V can be used for the research of cancer-related signaling pathways.

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Reinheit: 99.85%
CAS. Nr.: 64725-24-2
Formel: C₄₀H₄₈N₆O₉
Molecular Weight:756.84

Speicherung:

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

RA-V Chemical Structure

CAS. Nr.: 64725-24-2

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

IC50: 50 ng/mL (Wnt); 75 ng/mL (Myc), 93 ng/mL (Notch)^[1]

Alle Wnt Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.06 μM Compound: 7	Cytotoxicity against human A549 after 48 hrs by SRB assay Cytotoxicity against human A549 after 48 hrs by SRB assay	[PMID: 21044847]
B16	IC₅₀	0.001 μM Compound: 7	Cytotoxicity against mouse B16 after 48 hrs by SRB assay Cytotoxicity against mouse B16 after 48 hrs by SRB assay	[PMID: 21044847]
Bel-7402	IC₅₀	0.08 μM Compound: 7	Cytotoxicity against human Bel7402 after 48 hrs by SRB assay Cytotoxicity against human Bel7402 after 48 hrs by SRB assay	[PMID: 21044847]
BGC-823	IC₅₀	0.01 μM Compound: 7	Cytotoxicity against human BGC823 after 48 hrs by SRB assay Cytotoxicity against human BGC823 after 48 hrs by SRB assay	[PMID: 21044847]
DU-145	IC₅₀	0.01 μM Compound: 7	Cytotoxicity against human DU145 after 48 hrs by SRB assay Cytotoxicity against human DU145 after 48 hrs by SRB assay	[PMID: 21044847]
HEK293	IC₅₀	0.03 μM Compound: 7	Inhibition of NF-kappaB activity in TNF-alpha-induced human HEK293 cells after 30 mins by luciferase assay Inhibition of NF-kappaB activity in TNF-alpha-induced human HEK293 cells after 30 mins by luciferase assay	[PMID: 21044847]
HeLa	IC₅₀	0.04 μM Compound: 7	Cytotoxicity against human HeLa after 48 hrs by SRB assay Cytotoxicity against human HeLa after 48 hrs by SRB assay	[PMID: 21044847]
HepG2	IC₅₀	0.14 μM Compound: 7	Cytotoxicity against human HepG2 after 48 hrs by SRB assay Cytotoxicity against human HepG2 after 48 hrs by SRB assay	[PMID: 21044847]
MDA-MB-231	IC₅₀	0.01 μM Compound: 7	Cytotoxicity against human MDA-MB-231 after 48 hrs by SRB assay Cytotoxicity against human MDA-MB-231 after 48 hrs by SRB assay	[PMID: 21044847]
P388	IC₅₀	0.014 μg/mL Compound: 2	Cytotoxicity against mouse P388 cells Cytotoxicity against mouse P388 cells	[PMID: 18078747]
PC-3	IC₅₀	0.05 μM Compound: 7	Cytotoxicity against human PC3 after 48 hrs by SRB assay Cytotoxicity against human PC3 after 48 hrs by SRB assay	[PMID: 21044847]
RAW264.7	IC₅₀	0.05 μM Compound: 7	Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 18 hrs Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 18 hrs	[PMID: 21044847]
RAW264.7	IC₅₀	0.05 μM Compound: 7	Inhibition of NO production in Interferon gamma-stimulated mouse RAW264.7 cells after 18 hrs Inhibition of NO production in Interferon gamma-stimulated mouse RAW264.7 cells after 18 hrs	[PMID: 21044847]
SMMC-7721	IC₅₀	0.13 μM Compound: 7	Cytotoxicity against human SMMC7721 after 48 hrs by SRB assay Cytotoxicity against human SMMC7721 after 48 hrs by SRB assay	[PMID: 21044847]
U-251	IC₅₀	0.001 μM Compound: 7	Cytotoxicity against human U251 after 48 hrs by SRB assay Cytotoxicity against human U251 after 48 hrs by SRB assay	[PMID: 21044847]

In Vivo

RA-V has activity against Wnt, Myc and Notch with IC₅₀ values of 50, 75, and 93 ng/mL, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 64725-24-2
Appearance Solid
Molecular Weight 756.84
Formel C₄₀H₄₈N₆O₉
Color White to off-white
SMILES

OC1=CC=C2C=C1OC3=CC=C(C[C@@]4(N(C([C@H](C)NC([C@@H](N(C([C@H](C)NC([C@@H](C)NC([C@]([H])(C2)N(C)C4=O)=O)=O)=O)C)CC5=CC=C(OC)C=C5)=O)=O)C)[H])C=C3
Structure Classification
- Cyclopeptides
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (132.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.3213 mL

6.6064 mL

13.2128 mL

5 mM

0.2643 mL

1.3213 mL

2.6426 mL

10 mM

0.1321 mL

0.6606 mL

1.3213 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 2% DMSO 40% PEG300 5% Tween-80 53% Saline

Solubility: ≥ 1 mg/mL (1.32 mM); Clear solution

Protocol 2

Add each solvent one by one: 2% DMSO 98% (20% SBE-β-CD in Saline)

Solubility: ≥ 1 mg/mL (1.32 mM); Clear solution

Protocol 3

Add each solvent one by one: 2% DMSO 98% Corn Oil

Solubility: 1 mg/mL (1.32 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Premalatha Balachandran, et al. Crosstalk of Cancer Signaling Pathways by Cyclic Hexapeptides and Anthraquinones from Rubia cordifolia. Molecules. 2021 Jan 31;26(3):735. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.3213 mL	6.6064 mL	13.2128 mL	33.0321 mL
	5 mM	0.2643 mL	1.3213 mL	2.6426 mL	6.6064 mL
	10 mM	0.1321 mL	0.6606 mL	1.3213 mL	3.3032 mL
	15 mM	0.0881 mL	0.4404 mL	0.8809 mL	2.2021 mL
	20 mM	0.0661 mL	0.3303 mL	0.6606 mL	1.6516 mL
	25 mM	0.0529 mL	0.2643 mL	0.5285 mL	1.3213 mL
	30 mM	0.0440 mL	0.2202 mL	0.4404 mL	1.1011 mL
	40 mM	0.0330 mL	0.1652 mL	0.3303 mL	0.8258 mL
	50 mM	0.0264 mL	0.1321 mL	0.2643 mL	0.6606 mL
	60 mM	0.0220 mL	0.1101 mL	0.2202 mL	0.5505 mL
	80 mM	0.0165 mL	0.0826 mL	0.1652 mL	0.4129 mL
	100 mM	0.0132 mL	0.0661 mL	0.1321 mL	0.3303 mL

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RA-V

MCE Bioactive Compound Library

Natural Product Library Plus

Alle Wnt Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

RA-V

MCE Bioactive Compound Library

Natural Product Library Plus

Alle Wnt Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

RA-V Related Classifications

HY-N10790 Related Classifications