Dibenzothiophene
Dibenzothiophene is an orally active and a noncompetitive CYP1A inhibitor. Dibenzothiophene inhibits CYP1A-mediated EROD activity with a Km of 0.592 μM. Dibenzothiophene interacts with the AHR pathway. Dibenzothiophene enhances the embryotoxicity of β-naphthoflavone (HY-114740). Dibenzothiophene shows acute toxicity in mice. Dibenzothiophene is mainly used for the study of the mechanism of developmental toxicity in organisms.
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- Pureté: 99.92%
- CAS No.: 132-65-0
- Formule: C12H8S
- Masse moléculaire:184.26
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Stockage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
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CYP1 |
Dibenzothiophene (0-100 μM) inhibits Ethoxyresorufin-O-deethylase (EROD) activity in β-naphthoflavone-induced channel catfish microsomes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Dibenzothiophene (215-744 mg/kg; p.o., via esophageal tube, once) shows acute toxicity in male CD-1 mice[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male CD-1 mice (20-25 g, young adults)[2]
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Dosage:215, 265, 325, 400, 492, 605, 744 mg/kg (dissolved in corn oil)
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Administration:Oral gavage (p.o.), via esophageal tube, once
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Result:Caused abnormal behavior within 2 hr of ingestion, which varied from sluggishness to anesthesia.
Showed no hematological changes related to DBT.
Showed pulmonary congestion, edema, hydrothorax, intestinal hemorrhage, and liver lesions in dead mice.
Caused centrilobular liver necrosis, necrosis of thymic cortical lymphocytes, and degeneration of the pulmonary arteriolar wall.
Reduced LD50 by 29%.
Chemical Information
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CAS No. 132-65-0
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Appearance Solid
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Masse moléculaire 184.26
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Formule C12H8S
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Color White to light yellow
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SMILES
C12=CC=CC=C1C3=CC=CC=C3S2
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Synonyms
DBT; Diphenylene sulfide
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvant et solubilité
DMSO : 75 mg/mL (407.03 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 3.75 mg/mL (20.35 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 3.75 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 3.75 mg/mL (20.35 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 3.75 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (280 KB)
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SDS (597 KB)
- English - EN (597 KB)
- Français - FR (597 KB)
- Deutsch - DE (597 KB)
- Norwegian - NO (597 KB)
- Español - ES (597 KB)
- Swedish - SV (597 KB)
- Italian - IT (597 KB)
- Portuguese - PT (597 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Wassenberg DM, et al. Effects of the polycyclic aromatic hydrocarbon heterocycles, carbazole and dibenzothiophene, on in vivo and in vitro CYP1A activity and polycyclic aromatic hydrocarbon-derived embryonic deformities. Environ Toxicol Chem. 2005 Oct;24(10):2526-32. [Content Brief]
[2]. Leighton FA. Acute oral toxicity of dibenzothiophene for male CD-1 mice: LD50, lesions, and the effect of preinduction of mixed-function oxidases. Fundam Appl Toxicol. 1989 May;12(4):787-92. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.4271 mL | 27.1356 mL | 54.2711 mL | 135.6779 mL |
| 5 mM | 1.0854 mL | 5.4271 mL | 10.8542 mL | 27.1356 mL | |
| 10 mM | 0.5427 mL | 2.7136 mL | 5.4271 mL | 13.5678 mL | |
| 15 mM | 0.3618 mL | 1.8090 mL | 3.6181 mL | 9.0452 mL | |
| 20 mM | 0.2714 mL | 1.3568 mL | 2.7136 mL | 6.7839 mL | |
| 25 mM | 0.2171 mL | 1.0854 mL | 2.1708 mL | 5.4271 mL | |
| 30 mM | 0.1809 mL | 0.9045 mL | 1.8090 mL | 4.5226 mL | |
| 40 mM | 0.1357 mL | 0.6784 mL | 1.3568 mL | 3.3919 mL | |
| 50 mM | 0.1085 mL | 0.5427 mL | 1.0854 mL | 2.7136 mL | |
| 60 mM | 0.0905 mL | 0.4523 mL | 0.9045 mL | 2.2613 mL | |
| 80 mM | 0.0678 mL | 0.3392 mL | 0.6784 mL | 1.6960 mL | |
| 100 mM | 0.0543 mL | 0.2714 mL | 0.5427 mL | 1.3568 mL |
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