1. Epigenetics
    PI3K/Akt/mTOR
  2. AMPK
  3. COH-SR4

COH-SR4 

Cat. No.: HY-124822 Purity: 99.73%
Handling Instructions

COH-SR4 is an AMPK activator. COH-SR4 shows potent anti-proliferative activities against leukemia, melanoma, breast and lung cancers. COH-SR4 inhibits adipocyte differentiation via AMPK activation, and can be used for the research of obesity and related metabolic disorders.

For research use only. We do not sell to patients.

COH-SR4 Chemical Structure

COH-SR4 Chemical Structure

CAS No. : 73439-19-7

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25 mg USD 50 In-stock
Estimated Time of Arrival: December 31
50 mg USD 70 In-stock
Estimated Time of Arrival: December 31
100 mg USD 120 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

COH-SR4 is an AMPK activator. COH-SR4 shows potent anti-proliferative activities against leukemia, melanoma, breast and lung cancers. COH-SR4 inhibits adipocyte differentiation via AMPK activation, and can be used for the research of obesity and related metabolic disorders[1].

IC50 & Target

AMPK[1]

In Vitro

COH-SR4 (1-5 μM; 24 hours) results in a dose-dependent increase in the phosphorylation of AMPK and its substrate ACC in 3T3-L1 preadipocytes, as well as in cancer cells such as HL-60, HeLa, MCF-7[1].
COH-SR4 (3-5 µM; 7 days) significantly inhibits 3T3-L1 adipocyte differentiation in a dose-dependent manner[1].
COH-SR4 (1-5 μM; 24 hours) promotes cell G1 cycle arrest[1].
COH-SR4 significantly reduces intracellular lipid accumulation and downregulated the expression of key adipogenesis-related transcription factors and lipogenic proteins[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: 3T3-L1 preadipocytes, HL-60 cells, HeLa cells, MCF-7 cells
Concentration: 1 μM, 3 μM, 5 μM
Incubation Time: 24 hours
Result: Indirectly activated AMPK.

Cell Cycle Analysis[1]

Cell Line: 3T3-L1 cells
Concentration: 1 μM, 3 μM, 5 μM
Incubation Time: 24 hours
Result: Modulated the level of proteins active during S and G2 phases of the cell cycle.
In Vivo

COH-SR4 (5 mg/kg; i.g.; 3x/week; for 6 weeks) reduces body weight and fat mass in high fat diet (HFD) obese mice without affecting food intake[2].
COH-SR4 improves glycemic control and dyslipidemia in HFD obese mice[2].
COH-SR4 decreases adipose tissue hypertrophy and affects circulating adipokine levels in HFD obese mice[2].
COH-SR4 prevents hepatic lipid accumulation and fatty liver in HFD obese mice[2].
COH-SR4 regulates expression of genes involved in lipid and glucose metabolism[2].
COH-SR4 inhibits hepatic lipogenic and gluconeogenic pathways[2].
COH-SR4 indirectly activates AMPK independent of calcium signaling and LKB1 in vitro[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Nine-week old male C57BL/6J mice[2]
Dosage: 5 mg/kg
Administration: Oral gavage, three times a week, for 6 weeks
Result: Decreased body weight and fat mass in HFD obese mice.
Molecular Weight

350.03

Formula

C₁₃H₈Cl₄N₂O

CAS No.

73439-19-7

SMILES

O=C(NC1=CC(Cl)=CC(Cl)=C1)NC2=CC(Cl)=CC(Cl)=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (357.11 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8569 mL 14.2845 mL 28.5690 mL
5 mM 0.5714 mL 2.8569 mL 5.7138 mL
10 mM 0.2857 mL 1.4284 mL 2.8569 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 2.08 mg/mL (5.94 mM); Suspended solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (5.94 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

COH-SR4AMPKAMP-activated protein kinaseanticanceranti-proliferativeleukemiamelanomabreastlungcancersAMP-activatedproteinkinaseanti-adipogenicInhibitorinhibitorinhibit

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COH-SR4
Cat. No.:
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