2. Metabolic Enzyme/Protease
  3. Phospholipase
  4. Compound 48/80 trihydrochloride

Compound 48/80 trihydrochloride  (Synonyms: C48/80 trihydrochloride)

Cat. No.: HY-130592 Purity: 99.83%
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Compound 48/80 trihydrochloride (C48/80 trihydrochloride) is a mixture of condensation products of N-methyl-p-methoxyphenethylamine with formaldehyde. Compound 48/80 trihydrochloride is also a histamine releaser and a mast cell degranulator. Compound 48/80 inhibits phosphatidylinositol-specific phospholipase C activity from human platelets .

For research use only. We do not sell to patients.

CAS No. : 848035-21-2

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO In-stock
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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Compound 48/80 trihydrochloride:

Compound 48/80 trihydrochloride Related Antibodies

Top Publications Citing Use of Products

    Compound 48/80 trihydrochloride purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2025 Mar 10;43(3):446-463.e7.  [Abstract]

    Compound 48/80 trihydrochloride (5 mg/kg). The percentage of corresponding immune cells in a mouse 4T1 mammary tumor model was analyzed by flow cytometry.

    Compound 48/80 trihydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 May 19:159:114880.  [Abstract]

    Effects of Compound 48/80 (C48/80) (0.01 mg/kg, nasally administrated, at 10 min after HI) and cromolyn on the expression of IL-6 and TNF-α at 48 h after HI. Representative western blot bands of IL-6 and TNF-α in different groups.

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    • Customer Review

    Description

    Compound 48/80 trihydrochloride (C48/80 trihydrochloride) is a mixture of condensation products of N-methyl-p-methoxyphenethylamine with formaldehyde. Compound 48/80 trihydrochloride is also a histamine releaser and a mast cell degranulator. Compound 48/80 inhibits phosphatidylinositol-specific phospholipase C activity from human platelets [1][2][3].

    In Vitro

    Compound 48/80 trihydrochloride (C48/80 trihydrochloride) inhibits both cytosolic and particulate phosphatidylinositol-specific phospholipase C activities with a similar efficiency; IC50 values are 2.1 μg/ml (supernatant) and 5.0 μg /ml (particulate fraction). The aggregation of human platelets induced by ADP and PAF-acether is inhibited by Compound 48/80[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Compound 48/80 trihydrochloride Related Antibodies
    In Vivo

    Compound 48/80 trihydrochloride (0.75 mg/kg; i.p.; Killed after 0.5, 3 or 6 h) increases serum serotonin, histamine and corticosterone levels at 0.5 h, but their increases were reduced thereafter[3].
    Compound 48/80 trihydrochloride causes oxidative stress in rat adrenal gland through mast cell degranulation[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 7-week-old Wistar male rats (fasted for 24 hours)[3]
    Dosage: 0.75 mg/kg
    Administration: Intraperitoneal injection; Killed after 0.5, 3 or 6 h
    Result: Serum histamine and serotonin concentrations significantly higher than those in untreated control rats at 0.5 h after treatment. The increased serum histamine and serotonin concentrations in rats decreased time-dependently thereafter.
    Molecular Weight

    629.10

    Formula

    C32H48Cl3N3O3
    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    CNCCC1=CC(CC2=CC(CCNC)=CC=C2OC)=C(OC)C(CC3=CC(CCNC)=CC=C3OC)=C1.[H]Cl.[H]Cl.[H]Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (158.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 100 mg/mL (158.96 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.5896 mL 7.9479 mL 15.8957 mL
    5 mM 0.3179 mL 1.5896 mL 3.1791 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 5 mg/mL (7.95 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.83%

    SDS (420 KB)

    COA (252 KB) HNMR (211 KB) RP-HPLC (253 KB) LCMS (132 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 1.5896 mL 7.9479 mL 15.8957 mL 39.7393 mL
    5 mM 0.3179 mL 1.5896 mL 3.1791 mL 7.9479 mL
    10 mM 0.1590 mL 0.7948 mL 1.5896 mL 3.9739 mL
    15 mM 0.1060 mL 0.5299 mL 1.0597 mL 2.6493 mL
    20 mM 0.0795 mL 0.3974 mL 0.7948 mL 1.9870 mL
    25 mM 0.0636 mL 0.3179 mL 0.6358 mL 1.5896 mL
    30 mM 0.0530 mL 0.2649 mL 0.5299 mL 1.3246 mL
    40 mM 0.0397 mL 0.1987 mL 0.3974 mL 0.9935 mL
    50 mM 0.0318 mL 0.1590 mL 0.3179 mL 0.7948 mL
    60 mM 0.0265 mL 0.1325 mL 0.2649 mL 0.6623 mL
    80 mM 0.0199 mL 0.0993 mL 0.1987 mL 0.4967 mL
    100 mM 0.0159 mL 0.0795 mL 0.1590 mL 0.3974 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Compound 48/80 trihydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Compound 48/80 trihydrochloride848035-21-2C48/80 trihydrochloridePhospholipasehumanplateletaggregationhistamineoxidativestressInhibitorinhibitorinhibit

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