Compound 48/80 trihydrochloride
Based on 5 publication(s) in Google Scholar
Compound 48/80 trihydrochloride (C48/80 trihydrochloride) is a mixture of condensation products of N-methyl-p-methoxyphenethylamine with formaldehyde. Compound 48/80 trihydrochloride is also a histamine releaser and a mast cell degranulator. Compound 48/80 inhibits phosphatidylinositol-specific phospholipase C activity from human platelets .
For research use only. We do not sell to patients.
- Purity: 99.83%
- CAS No.: 848035-21-2
- Formula: C32H48Cl3N3O3
- Molecular Weight:629.10
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) Compound 48/80 trihydrochloride
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Customer Validation & Images
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Flow Cytometry
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All Phospholipase Isoforms
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Biological Activity
Compound 48/80 trihydrochloride (C48/80 trihydrochloride) inhibits both cytosolic and particulate phosphatidylinositol-specific phospholipase C activities with a similar efficiency; IC50 values are 2.1 μg/ml (supernatant) and 5.0 μg /ml (particulate fraction). The aggregation of human platelets induced by ADP and PAF-acether is inhibited by Compound 48/80[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Compound 48/80 trihydrochloride causes oxidative stress in rat adrenal gland through mast cell degranulation[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:7-week-old Wistar male rats (fasted for 24 hours)[3]
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Dosage:0.75 mg/kg
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Administration:Intraperitoneal injection; Killed after 0.5, 3 or 6 h
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Result:Serum histamine and serotonin concentrations significantly higher than those in untreated control rats at 0.5 h after treatment. The increased serum histamine and serotonin concentrations in rats decreased time-dependently thereafter.
Chemical Information
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CAS No. 848035-21-2
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Appearance Solid
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Molecular Weight 629.10
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Formula C32H48Cl3N3O3
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Color White to light yellow
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SMILES
CNCCC1=CC(CC2=CC(CCNC)=CC=C2OC)=C(OC)C(CC3=CC(CCNC)=CC=C3OC)=C1.[H]Cl.[H]Cl.[H]Cl
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Synonyms
C48/80 trihydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)
Publications (5)
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Journal Impact Factor
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Most Recent
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Cancer Cell
Distinct cellular mechanisms underlie chemotherapies and PD-L1 blockade combinations in triple-negative breast cancer. [Abstract]2025 Mar 10;43(3):446-463.e7. PMID: 39919737
Compound 48/80 trihydrochloride purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2025 Mar 10;43(3):446-463.e7. [Abstract]
Compound 48/80 trihydrochloride (5 mg/kg). The percentage of corresponding immune cells in a mouse 4T1 mammary tumor model was analyzed by flow cytometry.
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J Invest Dermatol
MRGPRX2-Mediated Mast Cell Activation Promotes Malignant Progression of Cutaneous Squamous Cell Carcinoma through IL-17A Release. [Abstract]2025 Nov 4:S0022-202X(25)03517-1. PMID: 41197765 -
Cells
Identification of Natural Compounds Triggering MRGPRX2-Mediated Calcium Flux and Degranulation in RBL-2H3 Cells. [Abstract]2026 Feb 3;15(3):287. PMID: 41677650 -
Int Immunopharmacol
Edaravone attenuates cerebral inflammation by inhibiting mast cells degranulation via ROS/STIM1 signaling pathway in HIE model. [Abstract]2025 May 19:159:114880. PMID: 40394799
Compound 48/80 trihydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 May 19:159:114880. [Abstract]
Effects of Compound 48/80 (C48/80) (0.01 mg/kg, nasally administrated, at 10 min after HI) and cromolyn on the expression of IL-6 and TNF-α at 48 h after HI. Representative western blot bands of IL-6 and TNF-α in different groups.
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Solvent & Solubility
DMSO : 100 mg/mL (158.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 100 mg/mL (158.96 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (7.95 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (420 KB)
- English - EN (420 KB)
- Français - FR (420 KB)
- Deutsch - DE (420 KB)
- Norwegian - NO (420 KB)
- Español - ES (420 KB)
- Swedish - SV (420 KB)
- Italian - IT (420 KB)
- Korean - KR (420 KB)
- Portuguese - PT (420 KB)
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Handling Instructions (2659 KB)
References
[1]. Bronner C, et al. Compound 48/80 is a potent inhibitor of phospholipase C and a dual modulator of phospholipase A2 from human platelet. Biochim Biophys Acta. 1987 Aug 15;920(3):301-5. [Content Brief]
[2]. Schemann M, et al. The mast cell degranulator compound 48/80 directly activates neurons. PLoS One. 2012;7(12):e52104. [Content Brief]
[3]. Kaida S, et al. Compound 48/80 causes oxidative stress in the adrenal gland of rats through mast cell degranulation. Free Radic Res. 2010 Feb;44(2):171-80. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 1.5896 mL | 7.9479 mL | 15.8957 mL | 39.7393 mL |
| 5 mM | 0.3179 mL | 1.5896 mL | 3.1791 mL | 7.9479 mL | |
| 10 mM | 0.1590 mL | 0.7948 mL | 1.5896 mL | 3.9739 mL | |
| 15 mM | 0.1060 mL | 0.5299 mL | 1.0597 mL | 2.6493 mL | |
| 20 mM | 0.0795 mL | 0.3974 mL | 0.7948 mL | 1.9870 mL | |
| 25 mM | 0.0636 mL | 0.3179 mL | 0.6358 mL | 1.5896 mL | |
| 30 mM | 0.0530 mL | 0.2649 mL | 0.5299 mL | 1.3246 mL | |
| 40 mM | 0.0397 mL | 0.1987 mL | 0.3974 mL | 0.9935 mL | |
| 50 mM | 0.0318 mL | 0.1590 mL | 0.3179 mL | 0.7948 mL | |
| 60 mM | 0.0265 mL | 0.1325 mL | 0.2649 mL | 0.6623 mL | |
| 80 mM | 0.0199 mL | 0.0993 mL | 0.1987 mL | 0.4967 mL | |
| 100 mM | 0.0159 mL | 0.0795 mL | 0.1590 mL | 0.3974 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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