Compound 48/80 (Synonyms: Poly-p-methoxyphenethylmethylamine)

Name: Compound 48/80
Brand: MedChemExpress
SKU: HY-115768
Rating: 5.0 (5 reviews)

Cat. No.: HY-115768 Purity: 99.98%: Data Sheet
COA

SDS
Handling Instructions
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Technical Support

Compound 48/80 (Poly-p-methoxyphenethylmethylamine) is widely used in animal and tissue models as a "selective" mast cell activator. Compound 48/80 acts at the mast cell membrane to stimulate trimeric G-proteins and induces degranulation via phospholipase C and D pathways.

For research use only. We do not sell to patients.

CAS No. : 94724-12-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Compound 48/80:

Compound 48/80 (trimer) In-stock
Compound 48/80 trihydrochloride In-stock

5 Publications Citing Use of MCE Compound 48/80

Flow Cytometry

: Compound 48/80 purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2025 Mar 10;43(3):446-463.e7. [Abstract]; Compound 48/80 trihydrochloride (5 mg/kg). The percentage of corresponding immune cells in a mouse 4T1 mammary tumor model was analyzed by flow cytometry.

: Compound 48/80 purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 May 19:159:114880. [Abstract]; Effects of Compound 48/80 (C48/80) (0.01 mg/kg, nasally administrated, at 10 min after HI) and cromolyn on the expression of IL-6 and TNF-α at 48 h after HI. Representative western blot bands of IL-6 and TNF-α in different groups.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Mast Cell Activator^[1]

In Vitro

Compound 48/80 (poly-p-methoxyphenethylmethylamine), an agent commonly used to trigger degranulation of mast cells, at concentrations of 5-20 μg/ml suppresses the proliferation of L1210 and Friend leukemic cells in vitro, inducing the formation of giant cells, which are polykaryons^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

519.72

Formula

C₃₂H₄₅N₃O₃

CAS No.

94724-12-6

Appearance

Solid

Color

White to light yellow

SMILES

CNCCC1=CC(CC2=CC(CCNC)=CC=C2OC)=C(OC)C(CC3=CC(CCNC)=CC=C3OC)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (96.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9241 mL	9.6206 mL	19.2411 mL
5 mM	0.3848 mL	1.9241 mL	3.8482 mL
10 mM	0.1924 mL	0.9621 mL	1.9241 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (4.81 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (4.81 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (4.81 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.98%

Select Batch:

Data Sheet (277 KB) SDS (252 KB)

COA (253 KB) HNMR (285 KB) LCMS (104 KB)

Handling Instructions (2659 KB)

References

[1]. Schemann M, et al. The mast cell degranulator compound 48/80 directly activates neurons. PLoS One. 2012;7(12):e52104. [Content Brief]

[2]. Wang YH, Taché Y, Harris AG, Kreutner W, Daly AF, Wei JY. Desloratadine prevents compound 48/80-induced mast cell degranulation: visualization using a vital fluorescent dye technique. Allergy. 2005;60(1):117-124. [Content Brief]

[3]. Darzynkiewicz Z, Carter S. Compound 48/80 impairs cytokinesis in murine leukemic cells. J Cell Physiol. 1984;119(1):1-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9241 mL	9.6206 mL	19.2411 mL	48.1028 mL
	5 mM	0.3848 mL	1.9241 mL	3.8482 mL	9.6206 mL
	10 mM	0.1924 mL	0.9621 mL	1.9241 mL	4.8103 mL
	15 mM	0.1283 mL	0.6414 mL	1.2827 mL	3.2069 mL
	20 mM	0.0962 mL	0.4810 mL	0.9621 mL	2.4051 mL
	25 mM	0.0770 mL	0.3848 mL	0.7696 mL	1.9241 mL
	30 mM	0.0641 mL	0.3207 mL	0.6414 mL	1.6034 mL
	40 mM	0.0481 mL	0.2405 mL	0.4810 mL	1.2026 mL
	50 mM	0.0385 mL	0.1924 mL	0.3848 mL	0.9621 mL
	60 mM	0.0321 mL	0.1603 mL	0.3207 mL	0.8017 mL
	80 mM	0.0241 mL	0.1203 mL	0.2405 mL	0.6013 mL

Compound 48/80 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Compound 48/8094724-12-6Poly-p-methoxyphenethylmethylaminePhospholipasemastcellactivatorG-proteinspolykaryonsproliferationleukemiccellsInhibitorinhibitorinhibit

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Compound 48/80 (Synonyms: Poly-p-methoxyphenethylmethylamine)

Customer Review

Other Forms of Compound 48/80:

Compound 48/80 Related Antibodies

5 Publications Citing Use of MCE Compound 48/80

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Phospholipase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Compound 48/80 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Compound 48/80 Related Classifications

Keywords:

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Customer Review

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