Compound 48/80
Based on 5 publication(s) in Google Scholar
Compound 48/80 (Poly-p-methoxyphenethylmethylamine) is widely used in animal and tissue models as a "selective" mast cell activator. Compound 48/80 acts at the mast cell membrane to stimulate trimeric G-proteins and induces degranulation via phospholipase C and D pathways.
For research use only. We do not sell to patients.
- Purity: 99.98%
- CAS No.: 94724-12-6
- Formula: C32H45N3O3
- Molecular Weight:519.72
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Compound 48/80
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Customer Validation & Images
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Flow Cytometry
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WB
All Phospholipase Isoforms
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Biological Activity
Mast Cell Activator[1]
Compound 48/80 (poly-p-methoxyphenethylmethylamine), an agent commonly used to trigger degranulation of mast cells, at concentrations of 5-20 μg/ml suppresses the proliferation of L1210 and Friend leukemic cells in vitro, inducing the formation of giant cells, which are polykaryons[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 94724-12-6
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Appearance Solid
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Molecular Weight 519.72
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Formula C32H45N3O3
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Color White to light yellow
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SMILES
CNCCC1=CC(CC2=CC(CCNC)=CC=C2OC)=C(OC)C(CC3=CC(CCNC)=CC=C3OC)=C1
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Synonyms
Poly-p-methoxyphenethylmethylamine
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (5)
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Journal Impact Factor
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Most Recent
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Cancer Cell
Distinct cellular mechanisms underlie chemotherapies and PD-L1 blockade combinations in triple-negative breast cancer. [Abstract]2025 Mar 10;43(3):446-463.e7. PMID: 39919737
Compound 48/80 purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2025 Mar 10;43(3):446-463.e7. [Abstract]
Compound 48/80 trihydrochloride (5 mg/kg). The percentage of corresponding immune cells in a mouse 4T1 mammary tumor model was analyzed by flow cytometry.
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J Invest Dermatol
MRGPRX2-Mediated Mast Cell Activation Promotes Malignant Progression of Cutaneous Squamous Cell Carcinoma through IL-17A Release. [Abstract]2025 Nov 4:S0022-202X(25)03517-1. PMID: 41197765 -
Cells
Identification of Natural Compounds Triggering MRGPRX2-Mediated Calcium Flux and Degranulation in RBL-2H3 Cells. [Abstract]2026 Feb 3;15(3):287. PMID: 41677650 -
Int Immunopharmacol
Edaravone attenuates cerebral inflammation by inhibiting mast cells degranulation via ROS/STIM1 signaling pathway in HIE model. [Abstract]2025 May 19:159:114880. PMID: 40394799
Compound 48/80 purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 May 19:159:114880. [Abstract]
Effects of Compound 48/80 (C48/80) (0.01 mg/kg, nasally administrated, at 10 min after HI) and cromolyn on the expression of IL-6 and TNF-α at 48 h after HI. Representative western blot bands of IL-6 and TNF-α in different groups.
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Solvent & Solubility
DMSO : 50 mg/mL (96.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (4.81 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (4.81 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Schemann M, et al. The mast cell degranulator compound 48/80 directly activates neurons. PLoS One. 2012;7(12):e52104. [Content Brief]
[2]. Wang YH, Taché Y, Harris AG, Kreutner W, Daly AF, Wei JY. Desloratadine prevents compound 48/80-induced mast cell degranulation: visualization using a vital fluorescent dye technique. Allergy. 2005;60(1):117-124. [Content Brief]
[3]. Darzynkiewicz Z, Carter S. Compound 48/80 impairs cytokinesis in murine leukemic cells. J Cell Physiol. 1984;119(1):1-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.9241 mL | 9.6206 mL | 19.2411 mL | 48.1028 mL |
| 5 mM | 0.3848 mL | 1.9241 mL | 3.8482 mL | 9.6206 mL | |
| 10 mM | 0.1924 mL | 0.9621 mL | 1.9241 mL | 4.8103 mL | |
| 15 mM | 0.1283 mL | 0.6414 mL | 1.2827 mL | 3.2069 mL | |
| 20 mM | 0.0962 mL | 0.4810 mL | 0.9621 mL | 2.4051 mL | |
| 25 mM | 0.0770 mL | 0.3848 mL | 0.7696 mL | 1.9241 mL | |
| 30 mM | 0.0641 mL | 0.3207 mL | 0.6414 mL | 1.6034 mL | |
| 40 mM | 0.0481 mL | 0.2405 mL | 0.4810 mL | 1.2026 mL | |
| 50 mM | 0.0385 mL | 0.1924 mL | 0.3848 mL | 0.9621 mL | |
| 60 mM | 0.0321 mL | 0.1603 mL | 0.3207 mL | 0.8017 mL | |
| 80 mM | 0.0241 mL | 0.1203 mL | 0.2405 mL | 0.6013 mL |
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