Cyanamide 

Cyanamide is a cell division and plant growth inhibitor, as well as an allelochemical derived from Vicia villosa. Cyanamide inhibits root growth and biomass accumulation in a dose-dependent manner by disrupting the formation of mitotic spindles and phragmoplast complexes, reducing the number of mitotic cells and blocking the cell cycle. The effects of Cyanamide are partially reversible after removal from low-concentration environments. Cyanamide is also a specific inhibitor of aldehyde dehydrogenase (ALDH). Although Cyanamide has no direct effect on tumor growth, it can significantly enhance the anti-tumor efficacy of Cyclophosphamide (HY-17420) at non-toxic doses by inhibiting the inactivation of Cyclophosphamide. Cyanamide enables Cyclophosphamide to exert equivalent therapeutic effects at lower doses, effectively inhibiting the growth of primary and metastatic tumors and prolonging the lifespan of tumor-bearing mice. Cyanamide is commonly used in studies related to ha-1 hepatoma and rls lymphosarcoma^[1][2].

Cyanamide (2-10 mM; 1-6 d) dose-dependently inhibits root growth, fresh weight accumulation, dry weight accumulation and RGR of bulb roots of onion (Allium cepa L.). Complete growth recovery is only observed after treatment with the low concentration (2 mM), while only partial or no recovery is observed after treatment with high concentrations^[1].
Cyanamide (2-10 mM; 1, 6 d) increases H₂O₂ levels in a dose- and time-dependent manner, inducing oxidative stress in bulb roots of onion (Allium cepa L.), with the highest concentration (10 mM) triggering a sharp rise in H₂O₂ after 1 day of treatment^[1].
Cyanamide (2-10 mM; 1-6 d) alters the accumulation of superoxide anions in the root of onion bulbs. Specifically, treatment with 10 mM cyanamide for 1 d induces extensive accumulation of superoxide anions, which subsequently triggers tissue degradation^[1].
Cyanamide (2-10 mM; 1-6 d) alters the mitotic index of onion root tip cells, disrupts chromosome morphology and metaphase plate formation, and all mitotic activity and chromosome structures recover completely after removal of this compound^[1].
Cyanamide (2-10 mM; 1-6 d) alters the cell cycle distribution of onion root tip cells, increases the proportion of 4C nuclei after short-term treatment, and elevates the G₂/G₁ ratio during the recovery phase^[1].
Cyanamide (2-10 mM; 1 d) disrupts the arrangement of microtubule cytoskeletons in onion root tip meristematic cells at concentrations of 6 mM and 10 mM, while no visible effect on microtubule structure is observed at the 2 mM concentration^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cyanamide (70 mg/kg; i.p.; 15 minutes prior to cyclophosphamide (HY-17420)) enhances cyclophosphamide's antitumor efficacy in murine hepatocellular carcinoma and lymphosarcoma models, increasing animal lifespan by at least 80% in the hepatocellular carcinoma model and allowing a 2/3 reduction in cyclophosphamide dose while maintaining equivalent effect in the lymphosarcoma model^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

42.04

CH₂N₂

420-04-2

Liquid (Density: 1.282 g/cm³)

N#CN

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

• [1]. Soltys D, et al. Cyanamide mode of action during inhibition of onion (Allium cepa L.) root growth involves disturbances in cell division and cytoskeleton formation. Planta. 2011;234(3):609-621.

  [2]. Kaledin V I, et al. Cyanamide promotes the antitumor effect of cyclophosphamide more than its toxic effect[C]//Doklady Biological Sciences. Springer Nature BV, 2008, 420(1): 195.