Cycloleucine
Based on 11 publication(s) in Google Scholar
Cycloleucine is a specific inhibitor of S-adenosyl-methionine mediated methylation. Cycloleucine is antagonist of NMDA receptor associated glycine receptor, with a Ki of 600 μM. Cycloleucine is also a competitive inhibitor of ATP: L-methionine-S-adenosyl transferase in vitro. Cycloleucine has anxiolytic and cytostatic effects.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 52-52-8
- Formula: C6H11NO2
- Molecular Weight:129.16
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Cycloleucine
More- Cancer Commun (Lond). 2025 Dec 25.
- Neuron. 2025 Nov 4:S0896-6273(25)00755-X. [Abstract]
- Int J Oral Sci.2024 May 23;16(1):42. [Abstract]
- Life Sci. 2024 Aug 12:122976. [Abstract]
- Int Immunopharmacol. 2026 Jun 15:179:116659. [Abstract]
- Poult Sci. 2022 Dec;101(12):102219. [Abstract]
- Cell Signal. 2023 Sep:109:110751. [Abstract]
- Acta Biochim Biophys Sin (Shanghai). 2025 Mar 19. [Abstract]
- Stem Cells Int. 2023 Jun 7:2023:8992284. [Abstract]
- Hum Cell. 2025 Nov 10;39(1):3. [Abstract]
- Int J Chron Obstruct Pulmon Dis. 2023 May 30:18:1007-1017. [Abstract]
Customer Validation & Images
-
RT-PCR
-
WB
-
In Vivo Efficacy Study
-
Cell Imaging/Staining
-
WB
All iGluR Isoforms
More
Biological Activity
|
NMDA Receptor |
Cycloleucine (4-40 mM; 3 h) blocks internal methylation of viral RNA in B77 transformed chick embryo fibroblasts[5].
Cycloleucine (40 mM; 24 h) blocks the formation of both m6A and the penultimate Gm in B77 38S RNA subunits by greater than 90%[5].
Cytostatic (10 μg/mL) inhibits the viability human KB and mouse L1210s leukemia cell lines[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Male rats bilaterally cannulated into the nucleus accumbens septi (NAS)[3]
-
Dosage:1 µL of 0.5, 1.0, 2.0, 4 µg/µL
-
Administration:Intracerebroventrical injection
-
Result:Increased time spent in the open arms and extreme arrivals at all doses.
Increased open arm entries at the dose of 4 μg.
Chemical Information
-
CAS No. 52-52-8
-
Appearance Solid
-
Molecular Weight 129.16
-
Formula C6H11NO2
-
Color White to off-white
-
SMILES
O=C(O)C1(CCCC1)N
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (11)
-
Journal Impact Factor
-
Most Recent
-
Cycloleucine purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 Dec 25.
The ratio of GAC/KGA in human HCC cell lines was detected using RT-qPCR after treatment with Cycloleucine (40 μM; 24 h).
Cycloleucine purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 Dec 25.
Cycloleucine (40 μM; 24 h) treatment promoted KGA expression and reduced GAC expression in human HCC cell lines as measured by Western blotting analysis.
-
Neuron
METTL14 integrates tumor-derived SAM to drive parabrachial epigenetic rewiring in pancreatic cancer. [Abstract]2025 Nov 4:S0896-6273(25)00755-X. PMID: 41192426
Cycloleucine purchased from MedChemExpress. Usage Cited in: Neuron. 2025 Nov 4:S0896-6273(25)00755-X. [Abstract]
Cycloleucine (cLEU) (4 μg/μL; 120 nL per side; i.c.) significantly alleviated abdominal pain, hunching behaviors, and depression-like behaviors in PDAC mice.
-
Int J Oral Sci
METTL7A-mediated m6A modification of corin reverses bisphosphonates-impaired osteogenic differentiation of orofacial BMSCs. [Abstract]2024 May 23;16(1):42. PMID: 38782892
Cycloleucine purchased from MedChemExpress. Usage Cited in: Int J Oral Sci.2024 May 23;16(1):42. [Abstract]
Treated with Cycloleucine (500 µg/mL), representative images and quantitative analyses of ALP and ARS staining of BMSCs from Vector/oe-CORIN Group.
Cycloleucine purchased from MedChemExpress. Usage Cited in: Int J Oral Sci.2024 May 23;16(1):42. [Abstract]
Cycloleucine (500 µg/mL) markedly decreased Runx2/OCN expression in BMSCs from the Vector/oe-CORIN group.
-
Life Sci
2024 Aug 12:122976. PMID: 39142507 -
Int Immunopharmacol
Keratinocyte-derived exosomal miR-31-5p reduces vemurafenib sensitivity in melanoma cells. [Abstract]2026 Jun 15:179:116659. PMID: 41997057 -
Poult Sci
2022 Dec;101(12):102219. PMID: 36308813 -
Cell Signal
FTO alleviates cerebral ischemia/reperfusion-induced neuroinflammation by decreasing cGAS mRNA stability in an m6A-dependent manner. [Abstract]2023 Sep:109:110751. PMID: 37321527 -
Acta Biochim Biophys Sin (Shanghai)
METTL3-mediated m 6A modification of pri-miRNA-31 promotes hypertrophic scar progression. [Abstract]2025 Mar 19. PMID: 40109092 -
Stem Cells Int
METTL3 Promotes Osteo/Odontogenic Differentiation of Stem Cells by Inhibiting miR-196b-5p Maturation. [Abstract]2023 Jun 7:2023:8992284. PMID: 37323630 -
Hum Cell
WTAP-mediated m6A modification of PTPN1 contributes to ulcerative colitis pathogenesis through regulating colonic epithelial cell phenotypes. [Abstract]2025 Nov 10;39(1):3. PMID: 41212293 -
Int J Chron Obstruct Pulmon Dis
Mettl3 Mediated m6A Methylation Involved in Epithelial-Mesenchymal Transition by Targeting SOCS3/STAT3/SNAI1 in Cigarette Smoking-Induced COPD. [Abstract]2023 May 30:18:1007-1017. PMID: 37275442
Solvent & Solubility
H2O : 20 mg/mL (154.85 mM; Need ultrasonic)
DMSO : < 1 mg/mL (insoluble or slightly soluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (774.23 mM); Clear solution; Need ultrasonic
Purity & Documentation
-
Data Sheet (278 KB)
-
SDS (479 KB)
- English - EN (479 KB)
- Français - FR (479 KB)
- Deutsch - DE (479 KB)
- Norwegian - NO (479 KB)
- Español - ES (479 KB)
- Swedish - SV (479 KB)
- Italian - IT (479 KB)
- Korean - KR (479 KB)
- Portuguese - PT (479 KB)
-
Handling Instructions (2659 KB)
References
[1]. Hood WF, et, al. 1-Aminocyclobutane-1-carboxylate (ACBC): a specific antagonist of the N-methyl-D-aspartate receptor coupled glycine receptor. Eur J Pharmacol. 1989 Feb 28;161(2-3):281-2. [Content Brief]
[2]. Caboche M, et, al. RNA methylation and control of eukaryotic RNA biosynthesis. Effects of cycloleucine, a specific inhibitor of methylation, on ribosomal RNA maturation. Eur J Biochem. 1977 Mar 15;74(1):19-29. [Content Brief]
[3]. Gargiulo API, et, al. Effects of Cycloleucine in the Nucleus Accumbens Septi on the Elevated plus Maze Test in Rats. Neuropsychobiology. 2020;79(3):191-197. [Content Brief]
[4]. Duś D, et, al. Cytostatic activity in vitro of cycloleucine, aspartic acid and glutamic acid phosphonic analogues. Arch Immunol Ther Exp (Warsz). 1980;28(3):433-8. [Content Brief]
[5]. Dimock K, et, al. Cycloleucine blocks 5'-terminal and internal methylations of avian sarcoma virus genome RNA. Biochemistry. 1978 Aug 22;17(17):3627-32. [Content Brief]
[6]. S Amor, et al. The effect of cycloleucine on SFV A7(74) infection in mice. Br J Exp Pathol. 1987 Apr;68(2):225-35. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 7.7423 mL | 38.7117 mL | 77.4233 mL | 193.5584 mL |
| 5 mM | 1.5485 mL | 7.7423 mL | 15.4847 mL | 38.7117 mL | |
| 10 mM | 0.7742 mL | 3.8712 mL | 7.7423 mL | 19.3558 mL | |
| 15 mM | 0.5162 mL | 2.5808 mL | 5.1616 mL | 12.9039 mL | |
| 20 mM | 0.3871 mL | 1.9356 mL | 3.8712 mL | 9.6779 mL | |
| 25 mM | 0.3097 mL | 1.5485 mL | 3.0969 mL | 7.7423 mL | |
| 30 mM | 0.2581 mL | 1.2904 mL | 2.5808 mL | 6.4519 mL | |
| 40 mM | 0.1936 mL | 0.9678 mL | 1.9356 mL | 4.8390 mL | |
| 50 mM | 0.1548 mL | 0.7742 mL | 1.5485 mL | 3.8712 mL | |
| 60 mM | 0.1290 mL | 0.6452 mL | 1.2904 mL | 3.2260 mL | |
| 80 mM | 0.0968 mL | 0.4839 mL | 0.9678 mL | 2.4195 mL | |
| 100 mM | 0.0774 mL | 0.3871 mL | 0.7742 mL | 1.9356 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Cycloleucine Related Classifications
HY-30008 Related Classifications
Powered by Bioz