Cat. No.: HY-N0391

L-Citrulline

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

L-Citrulline is an amino acid derived from ornithine in the catabolism of proline or glutamine and glutamate, or from l-arginine via arginine-citrulline pathway.

For research use only. We do not sell to patients.

Purity: 99.96%
CAS No.: 372-75-8
Formula: C₆H₁₃N₃O₃
Molecular Weight:175.19

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

L-Citrulline Chemical Structure

CAS No.: 372-75-8

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- Clinical Trial
Chemical Information
Publications (6)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

L-Citrulline is an amino acid derived from ornithine in the catabolism of proline or glutamine and glutamate, or from l-arginine via arginine-citrulline pathway.

IC₅₀ & Target

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

In Vitro

L-Citrulline is an amino acid derived from ornithine in the catabolism of proline or glutamine and glutamate, or from l-arginine via arginine-citrulline pathway. L-Citrulline is also obtained during the degradation of asymmetric dimethylarginine (ADMA), the process catalyzed by dimethylarginine dimethylaminohydrolase (DDAH), yielding dimethylamine (DMA) as a coproduct^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 372-75-8
Appearance Solid
Molecular Weight 175.19
Formula C₆H₁₃N₃O₃
Color White to off-white
SMILES

N[C@@H](CCCNC(N)=O)C(O)=O
Structure Classification
- Amino acids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (6)

All Cell Imaging/StainingIHCWBIFRT-PCR

Sort by:

Journal Impact Factor

Nature

Lithocholic acid phenocopies anti-ageing effects of calorie restriction. [Abstract]

2025 Jul;643(8070):192-200. PMID: 39695227
NPJ Biofilms Microbiomes

L-citrulline protects testicular Sertoli cell function by mitigating DNA damage via the gut-testis axis of sheep fed a high-concentrate diet. [Abstract]

2025 Oct 30;11(1):202. PMID: 41168195

L-Citrulline purchased from MedChemExpress. Usage Cited in: NPJ Biofilms Microbiomes. 2025 Oct 30;11(1):202. [Abstract]

L-Citrulline (Citr; 5 mM; 48 h). The representative images of ROS immunofluorescence of TM4 cell line in each group. Citr, treated with Citr; Sucrose, treated with Sucrose; Sucrose +H₂O₂, treated with Sucrose and Citr.

L-Citrulline purchased from MedChemExpress. Usage Cited in: NPJ Biofilms Microbiomes. 2025 Oct 30;11(1):202. [Abstract]

L-Citrulline (Citr; 5 mM; 48 h). Western blot of γH2AX and GAPDH in each indicated group.

L-Citrulline purchased from MedChemExpress. Usage Cited in: NPJ Biofilms Microbiomes. 2025 Oct 30;11(1):202. [Abstract]

L-Citrulline (Citr; 5 mM; 48 h). RT-qPCR analyzing the expression of Pcna and Gtf2h5 in each indicated group (n = 3).
Front Immunol

DMGV Is a Rheostat of T Cell Survival and a Potential Therapeutic for Inflammatory Diseases and Cancers. [Abstract]

2022 Aug 5:13:918241. PMID: 35990633
PLoS Biol

A high-throughput behavioral screening platform for measuring chemotaxis by C. elegans. [Abstract]

2024 Jun 27;22(6):e3002672. PMID: 38935621
Exp Ther Med

Citrulline protects against LPS‑induced acute lung injury by inhibiting ROS/NLRP3‑dependent pyroptosis and apoptosis via the Nrf2 signaling pathway. [Abstract]

2022 Aug 22;24(4):632. PMID: 36160882

L-Citrulline purchased from MedChemExpress. Usage Cited in: Exp Ther Med. 2022 Aug 22;24(4):632. [Abstract]

L-Citrulline (Cit; 1 g/kg/day; ip). Representative histological changes of lung tissue. Scale bar, 100 µm.

L-Citrulline purchased from MedChemExpress. Usage Cited in: Exp Ther Med. 2022 Aug 22;24(4):632. [Abstract]

L-Citrulline (Cit; 1 g/kg/day; ip). Representative macrophage infiltration shown as F4/80-positive cells. Sections were stained with primary antibodies against F4/80 (red), while the nucleus was stained with DAPI (blue). Scale bar, 100 µm. LPS, lipopolysaccharide; Con, control; Cit, citrulline.
bioRxiv

An efficient behavioral screening platform classifies natural products and other chemical cues according to their chemosensory valence in C. elegans. [Abstract]

2024 Apr 3:2023.06.02.542933. PMID: 37333363

Solvent & Solubility

In Vitro:

H₂O : ≥ 50 mg/mL (285.40 mM)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.7081 mL

28.5404 mL

57.0809 mL

5 mM

1.1416 mL

5.7081 mL

11.4162 mL

10 mM

0.5708 mL

2.8540 mL

5.7081 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (570.81 mM); Clear solution; Need ultrasonic

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Fleszar MG, et al. Quantitative Analysis of l-Arginine, Dimethylated Arginine Derivatives, l-Citrulline, and Dimethylamine in Human Serum Using Liquid Chromatography-Mass Spectrometric Method. Chromatographia. 2018;81(6):911-921. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	5.7081 mL	28.5404 mL	57.0809 mL	142.7022 mL
	5 mM	1.1416 mL	5.7081 mL	11.4162 mL	28.5404 mL
	10 mM	0.5708 mL	2.8540 mL	5.7081 mL	14.2702 mL
	15 mM	0.3805 mL	1.9027 mL	3.8054 mL	9.5135 mL
	20 mM	0.2854 mL	1.4270 mL	2.8540 mL	7.1351 mL
	25 mM	0.2283 mL	1.1416 mL	2.2832 mL	5.7081 mL
	30 mM	0.1903 mL	0.9513 mL	1.9027 mL	4.7567 mL
	40 mM	0.1427 mL	0.7135 mL	1.4270 mL	3.5676 mL
	50 mM	0.1142 mL	0.5708 mL	1.1416 mL	2.8540 mL
	60 mM	0.0951 mL	0.4757 mL	0.9513 mL	2.3784 mL
	80 mM	0.0714 mL	0.3568 mL	0.7135 mL	1.7838 mL
	100 mM	0.0571 mL	0.2854 mL	0.5708 mL	1.4270 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

L-Citrulline

Anti-COVID-19 Compound Library

Medicine Food Homology Compound Library

Orally Active Compound Library

FDA Approved & Pharmacopeial Drug Library

Food-Sourced Compound Library

Rare Diseases Drug Library

Pediatric Drug Library

Human Metabolite Library

Animal-Sourced Natural Product Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Drug Repurposing Compound Library Plus

Clinical Compound Library Plus

Bioactive Compound Library

RO5 Drug-like Natural Product Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Human Endogenous Metabolite Library

Mass Spectrometry Human Metabolite Library

Mouse Metabolite Compound Library

Cosmetic Ingredient Compound Library

Amino Acid Metabolite Compound Library

Mongolian Medicine Compound Library

Chinese Baijiu Compound Library

Tonifying Traditional Chinese Medicine Monomer Compound Library

Mass Spectrometry Natural Product Library

Natural Product and Natural Product-Like Compound Library

Metabolism/Protease Compound Library

Natural Product Library

Clinical Compound Library

Human Endogenous Metabolite Compound Library

Drug Repurposing Compound Library

Publications Citing Use of MedChemExpress (MCE) L-Citrulline

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (6)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

L-Citrulline Related Classifications

HY-N0391 Related Classifications