Diallyl disulfide
Based on 1 publication(s) in Google Scholar
Diallyl disulfide is an orally active decomposition product of allicin and has a pungent odor. Diallyl disulfide can act as a hydrogen sulfide donor. Diallyl disulfide has inhibitory activity against squalene monooxygenase with an IC50 of 400 μM. Diallyl disulfide can alleviate the cytotoxicity and apoptosis of intestinal and liver cells induced by Ethyl Carbamate (HY-B1207). Diallyl disulfide can prevent emphysema induced by cigarette smoke. Diallyl disulfide also has anti-colon cancer and anti-inflammatory neurogenic activities, and can reverse the depressive-like behavior of mice.
For research use only. We do not sell to patients.
- Purity: 99.8%
- CAS No.: 2179-57-9
- Formula: C6H10S2
- Molecular Weight:146.27
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Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Diallyl disulfide
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
100 μM
Compound: Diallyl disulfide
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Antiproliferative activity against human A549 cells
Antiproliferative activity against human A549 cells
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[PMID: 30245394] |
| Caco-2 | IC50 |
100 μM
Compound: Diallyl disulfide
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Antiproliferative activity against human Caco2 cells
Antiproliferative activity against human Caco2 cells
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[PMID: 30245394] |
| CHO | EC50 |
7.55 μM
Compound: 3
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Agonist activity at human TRPA1 channel expressed in CHO cells assessed as increase in intracellular calcium levels
Agonist activity at human TRPA1 channel expressed in CHO cells assessed as increase in intracellular calcium levels
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[PMID: 20356305] |
| DLD-1 | IC50 |
100 μM
Compound: Diallyl disulfide
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Antiproliferative activity against human DLD1 cells
Antiproliferative activity against human DLD1 cells
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[PMID: 30245394] |
| HCT-15 | IC50 |
100 μM
Compound: Diallyl disulfide
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Antiproliferative activity against human HCT15 cells
Antiproliferative activity against human HCT15 cells
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[PMID: 30245394] |
| MDCK | IC50 |
1.82 mM
Compound: 2
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Cytotoxicity against MDCK cells assessed as decrease in cell viability after 16 hrs by tetrazolium salt WST-1 assay
Cytotoxicity against MDCK cells assessed as decrease in cell viability after 16 hrs by tetrazolium salt WST-1 assay
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[PMID: 23163425] |
| SK-MEL-2 | IC50 |
100 μM
Compound: Diallyl disulfide
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Antiproliferative activity against human SK-MEL-2 cells
Antiproliferative activity against human SK-MEL-2 cells
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[PMID: 30245394] |
Diallyl disulfide (30-240 μM; 12-48 h) suppresses HT-29 cell growth[2].
Diallyl disulfide (30-120 μM; 24 h) induces cell cycle arrest in the G2/M-phase in HT-29 cells in a concentration-independent manner[2].
Diallyl disulfide (120 μM) induces the mRNA levels of ARPC3, SPINT2, SOD1, p21 and TACSTD2[2].
Diallyl disulfide (10-120 μM; 24 h) can protect Caco-2 and HepG2 cells from the cytotoxicity induced by ethyl carbamate. Compared with the group exposed to ethyl carbamate alone, it can significantly increase the cell survival rate[5].
Diallyl disulfide (10-40 μM; 24 h) can reduce the ROS production induced by carbamate exposure for 24 hours in Caco-2 and HepG2 cells[5].
Diallyl disulfide (10-40 μM; 24 h) can protect Caco-2 and HepG2 cells from the apoptosis and necrosis induced by carbamate. Compared with the group exposed to carbamate alone, it can significantly reduce the proportion of apoptotic and necrotic cells[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HT-29 cells
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Concentration:30, 60, 120 and 240 μM
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Incubation Time:12, 24, 48 h
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Result:Suppressed HT-29 cell growth by 5.3, 23.1, 45.6 and 68.3% with 30, 60, 120 and 240 µM for 24 h, respectively.
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Cell Line:HT-29 cells
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Concentration:30, 60, 120 μM
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Incubation Time:24 h
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Result:The percentage of cells in the G2/M-phase was found to be 23.6, 25.9, 39.7 and 61.0% with 30, 60, 120 and 240 µM, respectively.
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Cell Line:human colorectal adenocarcinoma (Caco-2) and hepatocarcinoma (HepG2) cells
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Concentration:10, 20 and 40 μM
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Incubation Time:24 h
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Result:Reduced ethyl carbamate-induced apoptosis (increased by 20% vs negative control) and necrosis (increased by 5% vs negative control) in Caco-2 cells by approximately 70% vs ethyl carbamate alone. Reduced ethyl carbamate-induced apoptosis and necrosis (increased by 10% vs negative control) in HepG2 cells compared to ethyl carbamate alone.
Did not induce apoptosis or necrosis in either cell line vs solvent control when used alone.
Diallyl disulfide (30-90 mg/kg; i.g.; three times/week for the last 30 days) dose-dependently reduces cigarette smoke (CS) -induced leukocyte infiltration into the airways and improves lung histology in C57BL/6 mice were exposed to CS for 60 consecutive days[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male C57BL6/J mice (6-8 weeks)[3]
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Dosage:40, 80 mg/kg
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Administration:IP; pre-treatment 1 h before a single LPS injection (100 μg/kg)
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Result:Prevented LPS-induced increases in immobility time in the TST and FST in mice.
Did not affect the locomotor activity in mice with or without LPS treatment.
Chemical Information
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CAS No. 2179-57-9
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Appearance Liquid (Density: 1.010 g/cm3)
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Molecular Weight 146.27
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Formula C6H10S2
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Color Colorless to light yellow
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SMILES
C=CCSSCC=C
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
DMSO : 100 mg/mL (683.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (17.09 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (17.09 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (481 KB)
- English - EN (481 KB)
- Français - FR (481 KB)
- Deutsch - DE (481 KB)
- Norwegian - NO (481 KB)
- Español - ES (481 KB)
- Swedish - SV (481 KB)
- Italian - IT (481 KB)
- Korean - KR (481 KB)
- Portuguese - PT (481 KB)
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Handling Instructions (2659 KB)
References
[1]. Gupta N, et al. Garlic and garlic-derived compounds inhibit human squalene monooxygenase. J Nutr. 2001;131(6):1662-1667. [Content Brief]
[2]. You-Sheng Huang, et al. Diallyl disulfide inhibits the proliferation of HT-29 human colon cancer cells by inducing differentially expressed genes. Mol Med Rep. 2011 May-Jun;4(3):553-9. [Content Brief]
[3]. Xiaoyou Wei, et al. Acute Diallyl Disulfide Administration Prevents and Reveres Lipopolysaccharide-Induced Depression-Like Behaviors in Mice via Regulating Neuroinflammation and Oxido-Nitrosative Stress. Inflammation. 2021 Aug;44(4):1381-1395. [Content Brief]
[4]. Aline de Oliveira Pontes Cardoso, et al. Diallyl disulfide prevents cigarette smoke-induced emphysema in mice. Pulm Pharmacol Ther. 2021 Aug:69:102053. [Content Brief]
[5]. Sanchez CA, et al. Diallyl Disulfide Reduces Ethyl Carbamate-Induced Cytotoxicity and Apoptosis in Intestinal and Hepatic Cells. Chem Res Toxicol. 2025 Apr 21;38(4):623-634. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 6.8367 mL | 34.1834 mL | 68.3667 mL | 170.9168 mL |
| 5 mM | 1.3673 mL | 6.8367 mL | 13.6733 mL | 34.1834 mL | |
| 10 mM | 0.6837 mL | 3.4183 mL | 6.8367 mL | 17.0917 mL | |
| 15 mM | 0.4558 mL | 2.2789 mL | 4.5578 mL | 11.3945 mL | |
| 20 mM | 0.3418 mL | 1.7092 mL | 3.4183 mL | 8.5458 mL | |
| 25 mM | 0.2735 mL | 1.3673 mL | 2.7347 mL | 6.8367 mL | |
| 30 mM | 0.2279 mL | 1.1394 mL | 2.2789 mL | 5.6972 mL | |
| 40 mM | 0.1709 mL | 0.8546 mL | 1.7092 mL | 4.2729 mL | |
| 50 mM | 0.1367 mL | 0.6837 mL | 1.3673 mL | 3.4183 mL | |
| 60 mM | 0.1139 mL | 0.5697 mL | 1.1394 mL | 2.8486 mL | |
| 80 mM | 0.0855 mL | 0.4273 mL | 0.8546 mL | 2.1365 mL | |
| 100 mM | 0.0684 mL | 0.3418 mL | 0.6837 mL | 1.7092 mL |
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