Molnupiravir (Synonyms: EIDD-2801; MK-4482)

Name: Molnupiravir
Brand: MedChemExpress
SKU: HY-135853
Rating: 5.0 (71 reviews)

Cat. No.: HY-135853 Purity: 99.85%: Data Sheet
COA

SDS
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Molnupiravir (EIDD-2801) is an orally bioavailable proagent of the ribonucleoside analog EIDD-1931. Molnupiravir has broad spectrum antiviral activity against influenza virus and multiple coronaviruses, such as SARS-CoV-2, MERS-CoV, SARS-CoV. Molnupiravir has the potential for the research of COVID-19, and seasonal and pandemic influenza.

For research use only. We do not sell to patients.

CAS No. : 2492423-29-5

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	Get quote
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Customer Review

Based on 71 publication(s) in Google Scholar

Other Forms of Molnupiravir:

Molnupiravir-d₇ In-stock
Molnupiravir (Standard) Get quote

71 Publications Citing Use of MCE Molnupiravir

In Vivo Efficacy Study

Bio/Physico-chemical Assay

IHC

: Molnupiravir purchased from MedChemExpress. Usage Cited in: Nature. 2022 Jul;607(7917):119-127. [Abstract]; Syrian hamsters were intranasally inoculated with 10³ PFU of BA.2 (NCD1288). One day after infection, hamsters were treated with: 500 mg/kg Molnupiravir, 1,000 mg/kg nirmatrelvir or 60 mg/kg S-217622 orally twice daily for 3 days. Methylcellulose served as a control for oral treatment. Eight hamsters per group were euthanized at 4 dpi for virus titration. Viral titres in the nasal turbinates and lungs were determined by plaque assay. For comparison of the lung and nasal turbinate titres of BA.2 (NCD1288)-and D614G (HP095)-infected hamster groups, we used a Kruskal-Wallis test with Dunn's multiple comparisons and one-way ANOVA with Dunnett's multiple comparisons, respectively. Data are from one experiment.

: Molnupiravir purchased from MedChemExpress. Usage Cited in: Nature. 2022 Apr;604(7904):134-140. [Abstract]; BLISS analysis in Calu-3 cells with molnupiravir or remdesivir in combination with the DHODH inhibitors Brequinar.

: Molnupiravir purchased from MedChemExpress. Usage Cited in: Nature. 2022 Apr;604(7904):134-140. [Abstract]; Wild-type BALB/C mice were treated with Brequinar (intraperitoneal administration) and/or Molnupiravir (oral administration) daily at the indicated concentrations starting 12 h before infection. Mice (n = 5 per group over 2 independent experiments) were intranasally inoculated with 1 × 10⁵ p.f.u. per mouse of SARS-CoV-2 (B.1.351). Lungs were analysed for viral titre 2 days after infection by plaque assay.

: Molnupiravir purchased from MedChemExpress. Usage Cited in: Nature. 2022 Apr;604(7904):134-140. [Abstract]; Wild-type BALB/C mice were treated with Brequinar (intraperitoneal administration) and/or Molnupiravir (oral administration) daily at the indicated concentrations starting 12 h before infection. Mice (n = 5 per group over 2 independent experiments) were intranasally inoculated with 1 × 10⁵ p.f.u. per mouse of SARS-CoV-2 (B.1.351). Fixed in 4% paraformaldehyde for haematoxylin and eosin staining and quantified for interstitial inflammation.

: Molnupiravir purchased from MedChemExpress. Usage Cited in: Nat Microbiol. 2022 Aug;7(8):1252-1258. [Abstract]; Syrian hamsters were intranasally inoculated with 10³ PFU of BA.1 (NC928). At 24 h post-infection, hamsters were treated with: 500 mg/kg Molnupiravir orally twice daily for 3 days or 60 mg/kg S-217622 orally twice daily for 3 days. Methylcellulose served as a control for oral treatment. Hamsters were euthanized on Days 2, 3, and 4 post-infection for virus titration. Vertical bars show the mean ± s.e.m. Points indicate data from individual hamsters (n = 4/group).

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
BHK-21	CC₅₀	42.25 μM Compound: 2; MK-4482	Cytotoxicity against hamster BHK-21 cells incubated for 4 days by MTS assay Cytotoxicity against hamster BHK-21 cells incubated for 4 days by MTS assay	[PMID: 36764470]
MDCK	CC₅₀	> 100 μM Compound: 2; MK-4482	Cytotoxicity against MDCK cells assessed as reduction in cell viability measured for 48 hrs by MTT assay Cytotoxicity against MDCK cells assessed as reduction in cell viability measured for 48 hrs by MTT assay	[PMID: 36764470]
NCI-H460	CC₅₀	18.3 μM Compound: MPV	Cytotoxicity against human NCI-H460 cells incubated for 72 hrs by CCK-8 assay Cytotoxicity against human NCI-H460 cells incubated for 72 hrs by CCK-8 assay	[PMID: 38061229]
Vero	CC₅₀	48.2 μM Compound: Molnupiravir	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell growth by coulter counter method Cytotoxicity against African green monkey Vero cells assessed as reduction in cell growth by coulter counter method	[PMID: 38232463]
Vero	CC₅₀	> 100 μM Compound: 2; MK-4482	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 36764470]

In Vivo

Molnupiravir (50-500 mg/kg; p.o.; every 12 hours for 3 days) is robustly antiviral and able to prevent SARS-CoV replication and disease^[1].
Molnupiravir (7 mg/kg; p.o.; twice daily for 3.5 days) significantly reduces shed virus load and duration of fever^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 mice (intranasal infection with SARS-CoV)^[1]
Dosage:	50, 150, 500 mg/kg
Administration:	Oral; every 12 hours for 3 days
Result:	Body weight loss is significantly diminished or prevented.

Animal Model:	Ca/09-infected female ferrets^[1]
Dosage:	7 mg/kg
Administration:	Oral; twice daily for 3.5 days
Result:	Shed virus load and duration of fever were significantly reduced.

Clinical Trial

Molecular Weight

329.31

Formula

C₁₃H₁₉N₃O₇

CAS No.

2492423-29-5

Appearance

Solid

Color

White to off-white

SMILES

O=C(N([C@@]1([H])[C@H](O)[C@H](O)[C@@]([H])(COC(C(C)C)=O)O1)C=C2)NC2=NO

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (151.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 12.5 mg/mL (37.96 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0367 mL	15.1833 mL	30.3665 mL
5 mM	0.6073 mL	3.0367 mL	6.0733 mL
10 mM	0.3037 mL	1.5183 mL	3.0367 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 25 mg/mL (75.92 mM); Clear solution; Need ultrasonic
Protocol 2

Add each solvent one by one: 10% PEG400 2.5% Ethoxylated hydrogenated castor oil 87.5% Water
Solubility: 12.05 mg/mL (36.59 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.94%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (253 KB) LCMS (92 KB) Elemental Analysis Report (110 KB)

Handling Instructions (2659 KB)

References

[1]. Sheahan TP, et al. An orally bioavailable broad-spectrum antiviral inhibits SARS-CoV-2 in human airway epithelial cell cultures and multiple coronaviruses in mice. Sci Transl Med. 2020 Apr 6. pii: eabb5883. [Content Brief]

[2]. Toots M, et al. Characterization of orally efficacious influenza drug with high resistance barrier in ferrets and human airway epithelia. Sci Transl Med. 2019 Oct 23;11(515). pii: eaax5866. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.0367 mL	15.1833 mL	30.3665 mL	75.9163 mL
	5 mM	0.6073 mL	3.0367 mL	6.0733 mL	15.1833 mL
	10 mM	0.3037 mL	1.5183 mL	3.0367 mL	7.5916 mL
	15 mM	0.2024 mL	1.0122 mL	2.0244 mL	5.0611 mL
	20 mM	0.1518 mL	0.7592 mL	1.5183 mL	3.7958 mL
	25 mM	0.1215 mL	0.6073 mL	1.2147 mL	3.0367 mL
	30 mM	0.1012 mL	0.5061 mL	1.0122 mL	2.5305 mL
DMSO	40 mM	0.0759 mL	0.3796 mL	0.7592 mL	1.8979 mL
	50 mM	0.0607 mL	0.3037 mL	0.6073 mL	1.5183 mL
	60 mM	0.0506 mL	0.2531 mL	0.5061 mL	1.2653 mL
	80 mM	0.0380 mL	0.1898 mL	0.3796 mL	0.9490 mL
	100 mM	0.0304 mL	0.1518 mL	0.3037 mL	0.7592 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molnupiravir Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Molnupiravir (Synonyms: EIDD-2801; MK-4482)

Customer Review

Other Forms of Molnupiravir:

Molnupiravir Related Antibodies

71 Publications Citing Use of MCE Molnupiravir

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Molnupiravir Related Classifications

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