Finasteride
Based on 4 publication(s) in Google Scholar
Finasteride (MK-906) is an orally active and competitive 5α-reductase inhibitor, with an IC50 of 4.2 nM for type II 5α-reductase. Finasteride has approximately a 100-fold greater affinity for type II 5α-reductase enzyme than for the type I enzyme. Finasteride can be used for the research of benign prostatic hyperplasia (BPH) and androgenic alopecia.
For research use only. We do not sell to patients.
- Purity: 99.97%
- CAS No.: 98319-26-7
- Formula: C23H36N2O2
- Molecular Weight:372.54
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
Publications Citing Use of MedChemExpress (MCE) Finasteride
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In Vivo Efficacy Study
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WB
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Cell Proliferation/Viability Assay
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In Vivo Efficacy Study
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In Vivo Efficacy Study
Biological Activity
IC50: 4.2 nM (type II 5α-reductase)[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CHO | IC50 |
911 nM
Compound: Finasteride
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Compound was evaluated for the inhibition of human Steroid 5-alpha-reductase type 1 from recombinant CHO cells
Compound was evaluated for the inhibition of human Steroid 5-alpha-reductase type 1 from recombinant CHO cells
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[PMID: 9873639] |
| DU-145 | IC50 |
0.04 μM
Compound: Finasteride
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Evaluated for the inhibitory activity against human steroid 5-alpha-reductase type I in human DU-145 cell assay at 5 nM of androstenedione
Evaluated for the inhibitory activity against human steroid 5-alpha-reductase type I in human DU-145 cell assay at 5 nM of androstenedione
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[PMID: 11063622] |
| DU-145 | IC50 |
60 nM
Compound: Finasteride
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Inhibition of [1-beta-3H]-androstenedione binding to human steroid 5-alpha-reductase type I expressed in DU-145 cells at 10 uM
Inhibition of [1-beta-3H]-androstenedione binding to human steroid 5-alpha-reductase type I expressed in DU-145 cells at 10 uM
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[PMID: 15828836] |
| DU-145 | IC50 |
3.9 μM
Compound: Finasteride
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Antiproliferative activity against human DU145 cells assessed as growth inhibition after 48 hrs by MTT assay
Antiproliferative activity against human DU145 cells assessed as growth inhibition after 48 hrs by MTT assay
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[PMID: 20171759] |
| DU-145 | IC50 |
548 μM
Compound: Finasteride
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Cytotoxicity against human DU145 cells by rapid colorimetric assay
Cytotoxicity against human DU145 cells by rapid colorimetric assay
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[PMID: 26780831] |
| DU-145 | IC50 |
13.53 μM
Compound: Finasteride
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Cytotoxicity against human DU145 cells assessed as growth inhibition after 24 hrs by CCK8 assay
Cytotoxicity against human DU145 cells assessed as growth inhibition after 24 hrs by CCK8 assay
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[PMID: 29615343] |
| DU-145 | IC50 |
14.53 μM
Compound: Finasteride
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Antiproliferative activity against human DU145 cells after 24 hrs by CCK-8 assay
Antiproliferative activity against human DU145 cells after 24 hrs by CCK-8 assay
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[PMID: 30482547] |
| DU-145 | IC50 |
26.3 nM
Compound: MK-906
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In vitro inhibition of human steroid 5-alpha-reductase type I in Du-145 cells
In vitro inhibition of human steroid 5-alpha-reductase type I in Du-145 cells
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[PMID: 7707319] |
| DU-145 | IC50 |
42 nM
Compound: Finasteride
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Inhibitory activity against human 5-alpha Reductase-1 expressed in DU-145 cells
Inhibitory activity against human 5-alpha Reductase-1 expressed in DU-145 cells
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[PMID: 9089333] |
| DU-145 | IC50 |
3.9 μM
Compound: Finasteride
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Cytotoxicity against Homo sapiens (human) DU145 cells after 48 hr by MTT assay
Cytotoxicity against Homo sapiens (human) DU145 cells after 48 hr by MTT assay
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10.1007/s00044-010-9393-3 |
| Fibroblast | IC50 |
62 nM
Compound: 1
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Compound was tested for inhibition of Type I 5-alpha-reductase in Human genital skin (Hs68) foreskin fibroblast cells.
Compound was tested for inhibition of Type I 5-alpha-reductase in Human genital skin (Hs68) foreskin fibroblast cells.
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[PMID: 8381185] |
| HEK293 | IC50 |
0.06 μM
Compound: Finasteride
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Inhibition of Human steroid 5-alpha-reductase type II expressed in HEK293 cells
Inhibition of Human steroid 5-alpha-reductase type II expressed in HEK293 cells
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[PMID: 11063622] |
| HEK293 | IC50 |
0.54 μM
Compound: Finasteride
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Inhibition of Human steroid 5-alpha-reductase type I expressed in HEK293 cells
Inhibition of Human steroid 5-alpha-reductase type I expressed in HEK293 cells
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[PMID: 11063622] |
| HEK293 | IC50 |
25 nM
Compound: 9f
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Inhibition of steroid 5-alpha reductase type 2 expressed in HEK 293 cells
Inhibition of steroid 5-alpha reductase type 2 expressed in HEK 293 cells
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[PMID: 17490876] |
| HEK293 | IC50 |
453 nM
Compound: 9f
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Inhibition of steroid 5-alpha reductase type 1 expressed in HEK 293 cells
Inhibition of steroid 5-alpha reductase type 1 expressed in HEK 293 cells
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[PMID: 17490876] |
| HEK293 | IC50 |
30.3 nM
Compound: 3
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Inhibition of human type-2 5-alpha reductase expressed in HEK293 cells using [3H]-androstenedione as substrate after 30 mins by HPLC analysis
Inhibition of human type-2 5-alpha reductase expressed in HEK293 cells using [3H]-androstenedione as substrate after 30 mins by HPLC analysis
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[PMID: 22776417] |
| HEK293 | IC50 |
453 nM
Compound: 3
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Inhibition of human type-1 5-alpha reductase expressed in HEK293 cells using [3H]-androstenedione as substrate after 30 mins by HPLC analysis
Inhibition of human type-1 5-alpha reductase expressed in HEK293 cells using [3H]-androstenedione as substrate after 30 mins by HPLC analysis
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[PMID: 22776417] |
| HEK293 | IC50 |
40 nM
Compound: Finasteride
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Inhibition of human 5alpha-2 reductase expressed in HEK293 cells assessed as suppression of conversion of [3]androstenedione incubated for 30 mins by radioactivity-based HPLC analysis
Inhibition of human 5alpha-2 reductase expressed in HEK293 cells assessed as suppression of conversion of [3]androstenedione incubated for 30 mins by radioactivity-based HPLC analysis
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[PMID: 26780831] |
| HEK293 | IC50 |
453 nM
Compound: Finasteride
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Inhibition of human 5alpha-1 reductase expressed in HEK293 cells assessed as suppression of conversion of [3]androstenedione incubated for 30 mins by radioactivity-based HPLC analysis
Inhibition of human 5alpha-1 reductase expressed in HEK293 cells assessed as suppression of conversion of [3]androstenedione incubated for 30 mins by radioactivity-based HPLC analysis
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[PMID: 26780831] |
| HEK293 | IC50 |
>50 μM
Compound: 1
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Cytotoxicity against HEK293 cells after 24 hrs by MTT assay
Cytotoxicity against HEK293 cells after 24 hrs by MTT assay
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[PMID: 28693914] |
| HEK293 | IC50 |
1.2 nM
Compound: 18
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Inhibition of human type 2 5alpha reductase expressed in HEK293 cells
Inhibition of human type 2 5alpha reductase expressed in HEK293 cells
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[PMID: 29400967] |
| HEK293 | IC50 |
650 nM
Compound: 18
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Inhibition of human type 1 5alpha reductase expressed in HEK293 cells
Inhibition of human type 1 5alpha reductase expressed in HEK293 cells
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[PMID: 29400967] |
| HEK293 | IC50 |
218 nM
Compound: 2a
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In vitro inhibition of human Steroid 5-alpha-reductase type I in transfected 293 cells using [3H]- delta4-Androstenedione as substrate
In vitro inhibition of human Steroid 5-alpha-reductase type I in transfected 293 cells using [3H]- delta4-Androstenedione as substrate
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[PMID: 7739004] |
| LNCaP | IC50 |
19.8 μg/mL
Compound: Finasteride
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Inhibitory activity against 5-alpha-reductase type 1 in cell culture system using LNCaP cells (androgen-sensitive human prostatic cancer cell line)
Inhibitory activity against 5-alpha-reductase type 1 in cell culture system using LNCaP cells (androgen-sensitive human prostatic cancer cell line)
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[PMID: 11527731] |
| LNCaP | IC50 |
53 μM
Compound: Finasteride
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Inhibitory activity against 5-alpha-reductase type 1 in cell culture system using LNCaP cells (androgen-sensitive human prostatic cancer cell line)
Inhibitory activity against 5-alpha-reductase type 1 in cell culture system using LNCaP cells (androgen-sensitive human prostatic cancer cell line)
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[PMID: 11527731] |
| LNCaP | IC50 |
14.53 μM
Compound: Finasteride
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Cytotoxicity against human LNCAP cells assessed as growth inhibition after 24 hrs by CCK8 assay
Cytotoxicity against human LNCAP cells assessed as growth inhibition after 24 hrs by CCK8 assay
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[PMID: 29615343] |
| LNCaP | IC50 |
13.53 μM
Compound: Finasteride
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Antiproliferative activity against human LNCAP cells after 24 hrs by CCK-8 assay
Antiproliferative activity against human LNCAP cells after 24 hrs by CCK-8 assay
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[PMID: 30482547] |
| LNCaP | IC50 |
0.76 μM
Compound: Finasteride
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Inhibition of steroid 5-alpha-reductase in human LNCAP cells assessed as reduction in 5- dihydrotestosterone formation incubated for 60 mins by LC-MS analysis
Inhibition of steroid 5-alpha-reductase in human LNCAP cells assessed as reduction in 5- dihydrotestosterone formation incubated for 60 mins by LC-MS analysis
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[PMID: 32040314] |
| Macrophage | IC50 |
28.2 μM
Compound: Finasteride
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Cytotoxicity against Balb/C mouse macrophages assessed as cell viability after 24 hrs by MTT assay
Cytotoxicity against Balb/C mouse macrophages assessed as cell viability after 24 hrs by MTT assay
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[PMID: 20171759] |
| MCF7 | IC50 |
44.14 μM
Compound: 1
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Cytotoxicity against human MCF7 cells after 24 hrs by MTT assay
Cytotoxicity against human MCF7 cells after 24 hrs by MTT assay
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[PMID: 28693914] |
| MDA-MB-231 | IC50 |
>50 μM
Compound: 1
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Cytotoxicity against human MDA-MB-231 cells after 24 hrs by MTT assay
Cytotoxicity against human MDA-MB-231 cells after 24 hrs by MTT assay
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[PMID: 28693914] |
| PC-3 | IC50 |
>80 μM
Compound: 1
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Cytotoxicity against human PC3 cells after 48 hrs by MTT assay
Cytotoxicity against human PC3 cells after 48 hrs by MTT assay
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[PMID: 28757062] |
| PC-3 | IC50 |
17.83 μM
Compound: Finasteride
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Cytotoxicity against human PC3 cells assessed as growth inhibition after 24 hrs by CCK8 assay
Cytotoxicity against human PC3 cells assessed as growth inhibition after 24 hrs by CCK8 assay
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[PMID: 29615343] |
| PC-3 | IC50 |
17.8 μM
Compound: Finasteride
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Antiproliferative activity against human PC3 cells after 24 hrs by CCK-8 assay
Antiproliferative activity against human PC3 cells after 24 hrs by CCK-8 assay
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[PMID: 30482547] |
| PC-3 | IC50 |
17.8 μM
Compound: Finasteride
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Antiproliferative activity against AR-deficient human PC3 cells assessed as reduction in cell viability after 24 hrs by CCK-8 assay
Antiproliferative activity against AR-deficient human PC3 cells assessed as reduction in cell viability after 24 hrs by CCK-8 assay
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[PMID: 31493989] |
Finasteride (10 μM; 6-24 h) induces the expression of HO-1 and Nrf2 proteins in PC-3 cells[2].
Finasteride decreases the conversion of [3H]testosterone (T) to [3H]dihydrotestosterone (DHT) in P. crustosum[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:PC-3, DU-145, and LNCaP cells
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Concentration:10 μM
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Incubation Time:6, 12, 24 h
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Result:Increased the expression of HO-1 protein in a time-dependent manner in PC-3 cells.
Induced the expression of Nrf2 protein in DU-145 and PC-3 cells, but not in LNCaP cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male dogs with spontaneous BPH (2.7-11 years old; 10.3-49 kg)[3]
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Dosage:0.1-0.5 mg/kg
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Administration:P.o. once daily for 16 weeks
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Result:Decreased prostatic diameter (20%), prostatic volume (43%), and serum DHT concentration (58%).
Decreased semen volume but did not adversely effect on semen quality or serum testosterone concentration.
No adverse effects on dogs.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 98319-26-7
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Appearance Solid
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Molecular Weight 372.54
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Formula C23H36N2O2
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Color White to off-white
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SMILES
O=C([C@H]1CC[C@]2([H])[C@]1(C)CC[C@]3([H])[C@@]4(C)C=CC(N[C@]4([H])CC[C@]32[H])=O)NC(C)(C)C
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Synonyms
MK-906
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (4)
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Journal Impact Factor
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Most Recent
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Phytomedicine
Bushen Tongluo formula ameliorated testosterone propionate-induced benign prostatic hyperplasia in rats. [Abstract]2023 Nov:120:155048. PMID: 37651753
Finasteride purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2023 Nov:120:155048. [Abstract]
Finasteride (Proscar) (0.5 mg/kg; i.g.; once daily for 28 d) significantly decreased the prostate volume, wet weight, and prostate index of TP-induced BPH rats.
Finasteride purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2023 Nov:120:155048. [Abstract]
Protein levels of TGF-β1, vimentin, and E-cadherin were detected by western blot analysis. Finasteride (Proscar) (0.5 mg/kg; i.g.; once daily for 28 d) significantly decreased TGF-β1 expression in the prostate tissues of BPH rats.
Finasteride purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2023 Nov:120:155048. [Abstract]
Finasteride (Proscar) (0.5 mg/kg; i.g.; once daily for 28 d)-spiked serum significantly inhibited the activities of BPH-1 cells at 48, 72, and 96 h compared to the serum of the model group.
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J Transl Med
The soluble epoxide hydrolase inhibitor TPPU improves comorbidity of chronic pain and depression via the AHR and TSPO signaling. [Abstract]2023 Feb 2;21(1):71. PMID: 36732752
Finasteride purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Feb 2;21(1):71. [Abstract]
Finasteride (Fina) (10 mg/kg; p.o.; once daily for 7 d) attenuated the analgesic effects of TPPU in anhedonia-susceptible mice.
Finasteride purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Feb 2;21(1):71. [Abstract]
TSPO antagonist Finasteride (Fina) (10 mg/kg; p.o.; once daily for 7 d) attenuated the antidepressant effects of TPPU in anhedonia-susceptible mice.
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J Pain
Alleviation of Mechanical Allodynia by 14,15-Epoxyeicosatrienoic Acid in a Central Poststroke Pain Model: Possible Role of Allopregnanolone and δ-Subunit-Containing Gamma-Aminobutyric Acid A Receptors. [Abstract]2019 May;20(5):577-591. PMID: 30500366 -
Sci Rep
Pao Pereira Extract Attenuates Testosterone-Induced Benign Prostatic Hyperplasia in Rats by inhibiting 5α-Reductase. [Abstract]2019 Dec 23;9(1):19703. PMID: 31873149
Solvent & Solubility
DMSO : 100 mg/mL (268.43 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.71 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 2 mg/mL (5.37 mM); Suspended solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (281 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Flores E, et, al. Steroid 5alpha-reductase inhibitors. Mini Rev Med Chem. 2003 May;3(3):225-37. [Content Brief]
[2]. Yun DK, et, al. Finasteride Increases the Expression of Hemoxygenase-1 (HO-1) and NF-E2-Related Factor-2 (Nrf2) Proteins in PC-3 Cells: Implication of Finasteride-Mediated High-Grade Prostate Tumor Occurrence. Biomol Ther (Seoul). 2013 Jan;21(1):49-53. [Content Brief]
[3]. Sirinarumitr K, et, al. Effects of finasteride on size of the prostate gland and semen quality in dogs with benign prostatic hypertrophy. J Am Vet Med Assoc. 2001 Apr 15;218(8):1275-80. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6843 mL | 13.4214 mL | 26.8428 mL | 67.1069 mL |
| 5 mM | 0.5369 mL | 2.6843 mL | 5.3686 mL | 13.4214 mL | |
| 10 mM | 0.2684 mL | 1.3421 mL | 2.6843 mL | 6.7107 mL | |
| 15 mM | 0.1790 mL | 0.8948 mL | 1.7895 mL | 4.4738 mL | |
| 20 mM | 0.1342 mL | 0.6711 mL | 1.3421 mL | 3.3553 mL | |
| 25 mM | 0.1074 mL | 0.5369 mL | 1.0737 mL | 2.6843 mL | |
| 30 mM | 0.0895 mL | 0.4474 mL | 0.8948 mL | 2.2369 mL | |
| 40 mM | 0.0671 mL | 0.3355 mL | 0.6711 mL | 1.6777 mL | |
| 50 mM | 0.0537 mL | 0.2684 mL | 0.5369 mL | 1.3421 mL | |
| 60 mM | 0.0447 mL | 0.2237 mL | 0.4474 mL | 1.1184 mL | |
| 80 mM | 0.0336 mL | 0.1678 mL | 0.3355 mL | 0.8388 mL | |
| 100 mM | 0.0268 mL | 0.1342 mL | 0.2684 mL | 0.6711 mL |