Finasteride (Synonyms: MK-906)

Name: Finasteride
Brand: MedChemExpress
SKU: HY-13635
Rating: 5.0 (4 reviews)

Cat. No.: HY-13635 Purity: 99.97%: Data Sheet
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Finasteride (MK-906) is an orally active and competitive 5α-reductase inhibitor, with an IC₅₀ of 4.2 nM for type II 5α-reductase. Finasteride has approximately a 100-fold greater affinity for type II 5α-reductase enzyme than for the type I enzyme. Finasteride can be used for the research of benign prostatic hyperplasia (BPH) and androgenic alopecia.

For research use only. We do not sell to patients.

CAS No. : 98319-26-7

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	Get quote
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Based on 4 publication(s) in Google Scholar

Other Forms of Finasteride:

4 Publications Citing Use of MCE Finasteride

In Vivo Efficacy Study

Cell Proliferation/Viability Assay

: Finasteride purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2023 Nov:120:155048.; Finasteride (Proscar) (0.5 mg/kg; i.g.; once daily for 28 d) significantly decreased the prostate volume, wet weight, and prostate index of TP-induced BPH rats.

: Finasteride purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2023 Nov:120:155048.; Protein levels of TGF-β1, vimentin, and E-cadherin were detected by western blot analysis. Finasteride (Proscar) (0.5 mg/kg; i.g.; once daily for 28 d) significantly decreased TGF-β1 expression in the prostate tissues of BPH rats.

: Finasteride purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2023 Nov:120:155048.; Finasteride (Proscar) (0.5 mg/kg; i.g.; once daily for 28 d)-spiked serum significantly inhibited the activities of BPH-1 cells at 48, 72, and 96 h compared to the serum of the model group.

: Finasteride purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Feb 2;21(1):71. [Abstract]; Finasteride (Fina) (10 mg/kg; p.o.; once daily for 7 d) attenuated the analgesic effects of TPPU in anhedonia-susceptible mice.

: Finasteride purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Feb 2;21(1):71. [Abstract]; TSPO antagonist Finasteride (Fina) (10 mg/kg; p.o.; once daily for 7 d) attenuated the antidepressant effects of TPPU in anhedonia-susceptible mice.

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Description

IC₅₀ & Target

IC50: 4.2 nM (type II 5α-reductase)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	911 nM Compound: Finasteride	Compound was evaluated for the inhibition of human Steroid 5-alpha-reductase type 1 from recombinant CHO cells Compound was evaluated for the inhibition of human Steroid 5-alpha-reductase type 1 from recombinant CHO cells	[PMID: 9873639]
DU-145	IC₅₀	0.04 μM Compound: Finasteride	Evaluated for the inhibitory activity against human steroid 5-alpha-reductase type I in human DU-145 cell assay at 5 nM of androstenedione Evaluated for the inhibitory activity against human steroid 5-alpha-reductase type I in human DU-145 cell assay at 5 nM of androstenedione	[PMID: 11063622]
DU-145	IC₅₀	13.53 μM Compound: Finasteride	Cytotoxicity against human DU145 cells assessed as growth inhibition after 24 hrs by CCK8 assay Cytotoxicity against human DU145 cells assessed as growth inhibition after 24 hrs by CCK8 assay	[PMID: 29615343]
DU-145	IC₅₀	14.53 μM Compound: Finasteride	Antiproliferative activity against human DU145 cells after 24 hrs by CCK-8 assay Antiproliferative activity against human DU145 cells after 24 hrs by CCK-8 assay	[PMID: 30482547]
DU-145	IC₅₀	26.3 nM Compound: MK-906	In vitro inhibition of human steroid 5-alpha-reductase type I in Du-145 cells In vitro inhibition of human steroid 5-alpha-reductase type I in Du-145 cells	[PMID: 7707319]
DU-145	IC₅₀	3.9 μM Compound: Finasteride	Cytotoxicity against Homo sapiens (human) DU145 cells after 48 hr by MTT assay Cytotoxicity against Homo sapiens (human) DU145 cells after 48 hr by MTT assay	10.1007/s00044-010-9393-3
DU-145	IC₅₀	3.9 μM Compound: Finasteride	Antiproliferative activity against human DU145 cells assessed as growth inhibition after 48 hrs by MTT assay Antiproliferative activity against human DU145 cells assessed as growth inhibition after 48 hrs by MTT assay	[PMID: 20171759]
DU-145	IC₅₀	42 nM Compound: Finasteride	Inhibitory activity against human 5-alpha Reductase-1 expressed in DU-145 cells Inhibitory activity against human 5-alpha Reductase-1 expressed in DU-145 cells	[PMID: 9089333]
DU-145	IC₅₀	548 μM Compound: Finasteride	Cytotoxicity against human DU145 cells by rapid colorimetric assay Cytotoxicity against human DU145 cells by rapid colorimetric assay	[PMID: 26780831]
DU-145	IC₅₀	60 nM Compound: Finasteride	Inhibition of [1-beta-3H]-androstenedione binding to human steroid 5-alpha-reductase type I expressed in DU-145 cells at 10 uM Inhibition of [1-beta-3H]-androstenedione binding to human steroid 5-alpha-reductase type I expressed in DU-145 cells at 10 uM	[PMID: 15828836]
Fibroblast	IC₅₀	62 nM Compound: 1	Compound was tested for inhibition of Type I 5-alpha-reductase in Human genital skin (Hs68) foreskin fibroblast cells. Compound was tested for inhibition of Type I 5-alpha-reductase in Human genital skin (Hs68) foreskin fibroblast cells.	[PMID: 8381185]
HEK293	IC₅₀	0.06 μM Compound: Finasteride	Inhibition of Human steroid 5-alpha-reductase type II expressed in HEK293 cells Inhibition of Human steroid 5-alpha-reductase type II expressed in HEK293 cells	[PMID: 11063622]
HEK293	IC₅₀	0.54 μM Compound: Finasteride	Inhibition of Human steroid 5-alpha-reductase type I expressed in HEK293 cells Inhibition of Human steroid 5-alpha-reductase type I expressed in HEK293 cells	[PMID: 11063622]
HEK293	IC₅₀	1.2 nM Compound: 18	Inhibition of human type 2 5alpha reductase expressed in HEK293 cells Inhibition of human type 2 5alpha reductase expressed in HEK293 cells	[PMID: 29400967]
HEK293	IC₅₀	218 nM Compound: 2a	In vitro inhibition of human Steroid 5-alpha-reductase type I in transfected 293 cells using [3H]- delta4-Androstenedione as substrate In vitro inhibition of human Steroid 5-alpha-reductase type I in transfected 293 cells using [3H]- delta4-Androstenedione as substrate	[PMID: 7739004]
HEK293	IC₅₀	25 nM Compound: 9f	Inhibition of steroid 5-alpha reductase type 2 expressed in HEK 293 cells Inhibition of steroid 5-alpha reductase type 2 expressed in HEK 293 cells	[PMID: 17490876]
HEK293	IC₅₀	30.3 nM Compound: 3	Inhibition of human type-2 5-alpha reductase expressed in HEK293 cells using [3H]-androstenedione as substrate after 30 mins by HPLC analysis Inhibition of human type-2 5-alpha reductase expressed in HEK293 cells using [3H]-androstenedione as substrate after 30 mins by HPLC analysis	[PMID: 22776417]
HEK293	IC₅₀	40 nM Compound: Finasteride	Inhibition of human 5alpha-2 reductase expressed in HEK293 cells assessed as suppression of conversion of [3]androstenedione incubated for 30 mins by radioactivity-based HPLC analysis Inhibition of human 5alpha-2 reductase expressed in HEK293 cells assessed as suppression of conversion of [3]androstenedione incubated for 30 mins by radioactivity-based HPLC analysis	[PMID: 26780831]
HEK293	IC₅₀	453 nM Compound: 3	Inhibition of human type-1 5-alpha reductase expressed in HEK293 cells using [3H]-androstenedione as substrate after 30 mins by HPLC analysis Inhibition of human type-1 5-alpha reductase expressed in HEK293 cells using [3H]-androstenedione as substrate after 30 mins by HPLC analysis	[PMID: 22776417]
HEK293	IC₅₀	453 nM Compound: 9f	Inhibition of steroid 5-alpha reductase type 1 expressed in HEK 293 cells Inhibition of steroid 5-alpha reductase type 1 expressed in HEK 293 cells	[PMID: 17490876]
HEK293	IC₅₀	453 nM Compound: Finasteride	Inhibition of human 5alpha-1 reductase expressed in HEK293 cells assessed as suppression of conversion of [3]androstenedione incubated for 30 mins by radioactivity-based HPLC analysis Inhibition of human 5alpha-1 reductase expressed in HEK293 cells assessed as suppression of conversion of [3]androstenedione incubated for 30 mins by radioactivity-based HPLC analysis	[PMID: 26780831]
HEK293	IC₅₀	650 nM Compound: 18	Inhibition of human type 1 5alpha reductase expressed in HEK293 cells Inhibition of human type 1 5alpha reductase expressed in HEK293 cells	[PMID: 29400967]
HEK293	IC₅₀	> 50 μM Compound: 1	Cytotoxicity against HEK293 cells after 24 hrs by MTT assay Cytotoxicity against HEK293 cells after 24 hrs by MTT assay	[PMID: 28693914]
LNCaP	IC₅₀	0.76 μM Compound: Finasteride	Inhibition of steroid 5-alpha-reductase in human LNCAP cells assessed as reduction in 5- dihydrotestosterone formation incubated for 60 mins by LC-MS analysis Inhibition of steroid 5-alpha-reductase in human LNCAP cells assessed as reduction in 5- dihydrotestosterone formation incubated for 60 mins by LC-MS analysis	[PMID: 32040314]
LNCaP	IC₅₀	13.53 μM Compound: Finasteride	Antiproliferative activity against human LNCAP cells after 24 hrs by CCK-8 assay Antiproliferative activity against human LNCAP cells after 24 hrs by CCK-8 assay	[PMID: 30482547]
LNCaP	IC₅₀	14.53 μM Compound: Finasteride	Cytotoxicity against human LNCAP cells assessed as growth inhibition after 24 hrs by CCK8 assay Cytotoxicity against human LNCAP cells assessed as growth inhibition after 24 hrs by CCK8 assay	[PMID: 29615343]
LNCaP	IC₅₀	19.8 μg/mL Compound: Finasteride	Inhibitory activity against 5-alpha-reductase type 1 in cell culture system using LNCaP cells (androgen-sensitive human prostatic cancer cell line) Inhibitory activity against 5-alpha-reductase type 1 in cell culture system using LNCaP cells (androgen-sensitive human prostatic cancer cell line)	[PMID: 11527731]
LNCaP	IC₅₀	53 μM Compound: Finasteride	Inhibitory activity against 5-alpha-reductase type 1 in cell culture system using LNCaP cells (androgen-sensitive human prostatic cancer cell line) Inhibitory activity against 5-alpha-reductase type 1 in cell culture system using LNCaP cells (androgen-sensitive human prostatic cancer cell line)	[PMID: 11527731]
MCF7	IC₅₀	44.14 μM Compound: 1	Cytotoxicity against human MCF7 cells after 24 hrs by MTT assay Cytotoxicity against human MCF7 cells after 24 hrs by MTT assay	[PMID: 28693914]
MDA-MB-231	IC₅₀	> 50 μM Compound: 1	Cytotoxicity against human MDA-MB-231 cells after 24 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells after 24 hrs by MTT assay	[PMID: 28693914]
Macrophage	IC₅₀	28.2 μM Compound: Finasteride	Cytotoxicity against Balb/C mouse macrophages assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against Balb/C mouse macrophages assessed as cell viability after 24 hrs by MTT assay	[PMID: 20171759]
PC-3	IC₅₀	17.8 μM Compound: Finasteride	Antiproliferative activity against human PC3 cells after 24 hrs by CCK-8 assay Antiproliferative activity against human PC3 cells after 24 hrs by CCK-8 assay	[PMID: 30482547]
PC-3	IC₅₀	17.8 μM Compound: Finasteride	Antiproliferative activity against AR-deficient human PC3 cells assessed as reduction in cell viability after 24 hrs by CCK-8 assay Antiproliferative activity against AR-deficient human PC3 cells assessed as reduction in cell viability after 24 hrs by CCK-8 assay	[PMID: 31493989]
PC-3	IC₅₀	17.83 μM Compound: Finasteride	Cytotoxicity against human PC3 cells assessed as growth inhibition after 24 hrs by CCK8 assay Cytotoxicity against human PC3 cells assessed as growth inhibition after 24 hrs by CCK8 assay	[PMID: 29615343]
PC-3	IC₅₀	> 80 μM Compound: 1	Cytotoxicity against human PC3 cells after 48 hrs by MTT assay Cytotoxicity against human PC3 cells after 48 hrs by MTT assay	[PMID: 28757062]

In Vitro

Finasteride (10 μM; 6-24 h) induces the expression of HO-1 and Nrf2 proteins in PC-3 cells^[2].
Finasteride decreases the conversion of [³H]testosterone (T) to [³H]dihydrotestosterone (DHT) in P. crustosum^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:	PC-3, DU-145, and LNCaP cells
Concentration:	10 μM
Incubation Time:	6, 12, 24 h
Result:	Increased the expression of HO-1 protein in a time-dependent manner in PC-3 cells. Induced the expression of Nrf2 protein in DU-145 and PC-3 cells, but not in LNCaP cells.

In Vivo

Finasteride (0.1-0.5 mg/kg; p.o. once daily for 16 weeks) reduces prostatic size in dogs with BPH without adversely affecting semen quality or serum testosterone concentration^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male dogs with spontaneous BPH (2.7-11 years old; 10.3-49 kg)^[3]
Dosage:	0.1-0.5 mg/kg
Administration:	P.o. once daily for 16 weeks
Result:	Decreased prostatic diameter (20%), prostatic volume (43%), and serum DHT concentration (58%). Decreased semen volume but did not adversely effect on semen quality or serum testosterone concentration. No adverse effects on dogs.

Clinical Trial

Molecular Weight

372.54

Formula

C₂₃H₃₆N₂O₂

CAS No.

98319-26-7

Appearance

Solid

Color

White to off-white

SMILES

O=C([C@H]1CC[C@]2([H])[C@]1(C)CC[C@]3([H])[C@@]4(C)C=CC(N[C@]4([H])CC[C@]32[H])=O)NC(C)(C)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (268.43 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6843 mL	13.4214 mL	26.8428 mL
5 mM	0.5369 mL	2.6843 mL	5.3686 mL
10 mM	0.2684 mL	1.3421 mL	2.6843 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.71 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.71 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 2 mg/mL (5.37 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.97%

Select Batch:

Data Sheet (281 KB) SDS (393 KB)

COA (254 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Flores E, et, al. Steroid 5alpha-reductase inhibitors. Mini Rev Med Chem. 2003 May;3(3):225-37. [Content Brief]

[2]. Yun DK, et, al. Finasteride Increases the Expression of Hemoxygenase-1 (HO-1) and NF-E2-Related Factor-2 (Nrf2) Proteins in PC-3 Cells: Implication of Finasteride-Mediated High-Grade Prostate Tumor Occurrence. Biomol Ther (Seoul). 2013 Jan;21(1):49-53. [Content Brief]

[3]. Sirinarumitr K, et, al. Effects of finasteride on size of the prostate gland and semen quality in dogs with benign prostatic hypertrophy. J Am Vet Med Assoc. 2001 Apr 15;218(8):1275-80. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6843 mL	13.4214 mL	26.8428 mL	67.1069 mL
	5 mM	0.5369 mL	2.6843 mL	5.3686 mL	13.4214 mL
	10 mM	0.2684 mL	1.3421 mL	2.6843 mL	6.7107 mL
	15 mM	0.1790 mL	0.8948 mL	1.7895 mL	4.4738 mL
	20 mM	0.1342 mL	0.6711 mL	1.3421 mL	3.3553 mL
	25 mM	0.1074 mL	0.5369 mL	1.0737 mL	2.6843 mL
	30 mM	0.0895 mL	0.4474 mL	0.8948 mL	2.2369 mL
	40 mM	0.0671 mL	0.3355 mL	0.6711 mL	1.6777 mL
	50 mM	0.0537 mL	0.2684 mL	0.5369 mL	1.3421 mL
	60 mM	0.0447 mL	0.2237 mL	0.4474 mL	1.1184 mL
	80 mM	0.0336 mL	0.1678 mL	0.3355 mL	0.8388 mL
	100 mM	0.0268 mL	0.1342 mL	0.2684 mL	0.6711 mL

Finasteride Related Classifications

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Finasteride (Synonyms: MK-906)

Customer Review

Other Forms of Finasteride:

4 Publications Citing Use of MCE Finasteride

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Finasteride (Synonyms: MK-906)

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Finasteride Related Antibodies

4 Publications Citing Use of MCE Finasteride

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Biological Activity

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References

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