2. Vitamin D Related/Nuclear Receptor
  3. Estrogen Receptor/ERR
  4. FLTX1

FLTX1 is a fluorescent Tamoxifen derivative that can specifically label intracellular Tamoxifen-binding sites (estrogen receptors) under permeabilized and non-permeabilized conditions. FLTX1 exhibits the potent antiestrogenic properties of Tamoxifen in breast cancer cells. FLTX1 is devoid of the estrogenic agonistic effect on the uterus.

For research use only. We do not sell to patients.

FLTX1 Chemical Structure

FLTX1 Chemical Structure

CAS No. : 1481401-71-1

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 352 In-stock
Solution
10 mM * 1 mL in DMSO USD 352 In-stock
Solid
5 mg USD 320 In-stock
10 mg USD 500 In-stock
25 mg USD 1050 In-stock
50 mg USD 1650 In-stock
100 mg USD 2600 In-stock
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500 mg   Get quote  

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Based on 2 publication(s) in Google Scholar

FLTX1 Related Antibodies

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  • Biological Activity

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Description

FLTX1 is a fluorescent Tamoxifen derivative that can specifically label intracellular Tamoxifen-binding sites (estrogen receptors) under permeabilized and non-permeabilized conditions. FLTX1 exhibits the potent antiestrogenic properties of Tamoxifen in breast cancer cells. FLTX1 is devoid of the estrogenic agonistic effect on the uterus[1][2].

IC50 & Target

ERα

87.5 nM (IC50)

In Vitro

FLTX1 (0.01-10 μM; 6 d) reduces MCF7 cell proliferation in a dose-dependent manner. FLTX1 (pretreated 24 h) counteracts the increase in cell growth induced by E2 down to the vehicle level[1].
FLTX1 (50 μM; 2 h) exhibits a dose-dependent competition with Tamoxifen (Tx) in MCF7 cells[1].
FLTX1 (0.1 nM-100 μM; 18 h) competitively displaces the [3H] E2 binding to rat uterine estrogen receptors (ER) rat uterus cytosol, with an IC50 of 87.5 nM[1].
FLTX1 (0.1 nM-10 μM; pretreated 8 h) reduces the estradiol-induced luciferase expression activity in a dose-dependent manner. FLTX1 (15-16 h) is devoid of the potent estrogenic agonist activity in both transiently transfected MCF7 cells and stably transfected T47D-KBluc cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

FLTX1 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: MCF7 cells
Concentration: 0.01, 0.1, 1, 5, 10 μM
Incubation Time: 6 days
Result: Inhibited MCF7 cell proliferation in a dose-dependent manner, being significantly more effective than Tx already at 0.1 μM.
In Vivo

FLTX1 (0.01-1 mg/kg/d; s.c. for 3 d) is lacked of the estrogenic uterotrophic (and also cervical and vaginal), hyperplasic and hypertrophic effects, and failed to alter basal proliferating cell nuclear antigen immunoreactivity in mice and rats[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

520.59

Formula

C31H28N4O4
CAS No.
Appearance

Solid

Color

Orange to red

SMILES

O=[N+](C1=CC=C(N(CCOC2=CC=C(/C(C3=CC=CC=C3)=C(C4=CC=CC=C4)/CC)C=C2)C)C5=NON=C51)[O-].[(Z)]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (48.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9209 mL 9.6045 mL 19.2090 mL
5 mM 0.3842 mL 1.9209 mL 3.8418 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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mg/kg

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g

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(per animal)

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.78%

COA (250 KB) RP-HPLC (241 KB) MS (70 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9209 mL 9.6045 mL 19.2090 mL 48.0224 mL
5 mM 0.3842 mL 1.9209 mL 3.8418 mL 9.6045 mL
10 mM 0.1921 mL 0.9604 mL 1.9209 mL 4.8022 mL
15 mM 0.1281 mL 0.6403 mL 1.2806 mL 3.2015 mL
20 mM 0.0960 mL 0.4802 mL 0.9604 mL 2.4011 mL
25 mM 0.0768 mL 0.3842 mL 0.7684 mL 1.9209 mL
30 mM 0.0640 mL 0.3201 mL 0.6403 mL 1.6007 mL
40 mM 0.0480 mL 0.2401 mL 0.4802 mL 1.2006 mL
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FLTX1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

FLTX11481401-71-1FLTX 1FLTX-1Estrogen Receptor/ERRfluorescentTamoxifenestrogenreceptorERpermeabilizednon-permeabilizedantiestrogenicbreastcanceruterusInhibitorinhibitorinhibit

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