Glycerophosphocholine (Synonyms: (S)-Glycerolphosphocholine)

Cat. No.: HY-W096638A Purity: 98.89%: Data Sheet
COA

SDS
Handling Instructions
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Glycerophosphocholine ((S)-Glycerolphosphocholine) is an orally active metabolite that crosses the blood-brain barrier. Glycerophosphocholine acts as a precursor of acetylcholine, a specific marker for phosphatidylcholine breakdown, a major water-soluble storage form of choline, and an intermediate in phosphatidylcholine metabolism. Glycerophosphocholine is specifically elevated in the cerebrospinal fluid of Alzheimer's disease models, and it also promotes growth hormone secretion and fat oxidation. Glycerophosphocholine is also a potential biomarker for breast cancer.

For research use only. We do not sell to patients.

Glycerophosphocholine Chemical Structure

CAS No. : 4217-84-9

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	Get quote
50 mg	Get quote

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Other Forms of Glycerophosphocholine:

Glycerylphosphorylcholine Get quote

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

Glycerophosphocholine can be hydrolyzed to choline by GPCPD1 both inside and outside Caco-2 cells, thereby regulating choline homeostasis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Glycerophosphocholine (500 mg/kg; oral gavage; single dose) increases circulating choline, betaine, and TMAO levels in healthy 6-week-old male C57BL/6J mice, with peak TMAO elevation occurring 3 to 6 hours post-dose^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6J (6-week-old, male)^[1]
Dosage:	500 mg/kg
Administration:	oral gavage; single dose
Result:	Increased blood glycerophosphocholine (GPC) and choline concentrations rapidly at 0.5 hours post-administration. Increased blood trimethylamine N-oxide (TMAO) levels at 3 and 6 hours post-administration. Increased blood betaine concentrations at 3 and 6 hours post-administration.

Molecular Weight

257.22

Formula

C₈H₂₀NO₆P

CAS No.

4217-84-9

Appearance

Solid

Color

White to off-white

SMILES

C[N+](C)(CCOP([O-])(OC[C@H](CO)O)=O)C

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 2.5 mg/mL (9.72 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8877 mL	19.4386 mL	38.8772 mL
5 mM	0.7775 mL	3.8877 mL	7.7754 mL
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.89%

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Data Sheet (271 KB) SDS (393 KB)

COA (244 KB)

Handling Instructions (2659 KB)

References

[1]. Chen S, et al. Role of Gpcpd1 in intestinal alpha-glycerophosphocholine metabolism and trimethylamine N-oxide production. J Biol Chem. 2024;300(12):107965. [Content Brief]

[2]. Moestue SA, et al. Glycerophosphocholine (GPC) is a poorly understood biomarker in breast cancer. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):E2506; author reply E2507. [Content Brief]

[3]. Kawamura T, et al. Glycerophosphocholine enhances growth hormone secretion and fat oxidation in young adults. Nutrition. 2012;28(11-12):1122-1126. [Content Brief]

[4]. Walter A, et al. Glycerophosphocholine is elevated in cerebrospinal fluid of Alzheimer patients. Neurobiol Aging. 2004 Nov-Dec;25(10):1299-303. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	3.8877 mL	19.4386 mL	38.8772 mL	97.1931 mL
H₂O	5 mM	0.7775 mL	3.8877 mL	7.7754 mL	19.4386 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Glycerophosphocholine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Glycerophosphocholine4217-84-9(S)-GlycerolphosphocholineEndogenous Metaboliteatherosclerosiscerebrospinal fluidC57BL/6J miceGlycerophosphodiesterase 1Alzheimer’s diseaseneurodegenerative disordershippocampal acetylcholineCaco-2 cell monolayersstrokebreast cancerInhibitorinhibitorinhibit

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