1. Protein Tyrosine Kinase/RTK
  2. FAK
  3. Harringtonolide

Harringtonolide is a potent RACK1 inhibitor (IC50=39.66 μM in A375 cells). Harringtonolide inhibits the epithelial-mesenchymal transition (EMT) process and cell proliferation by affecting the interaction between FAK and RACK1. Harringtonolide has plant growth inhibitory, antiviral, anti-inflammatory, and antiproliferation activities.

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Harringtonolide Chemical Structure

Harringtonolide Chemical Structure

CAS No. : 64761-48-4

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Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Harringtonolide

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Description

Harringtonolide is a potent RACK1 inhibitor (IC50=39.66 μM in A375 cells). Harringtonolide inhibits the epithelial-mesenchymal transition (EMT) process and cell proliferation by affecting the interaction between FAK and RACK1. Harringtonolide has plant growth inhibitory, antiviral, anti-inflammatory, and antiproliferation activities[1].

IC50 & Target

IC50: 39.66 μM (RACK1) in A375 cells[1]

In Vitro

Harringtonolide (0-50 μM; 24 hours) exhibits good antiproliferation activity in A375 cells with IC50 of 39.66 μM[1].
Harringtonolide (0-20 μM; 1 hour) restrains the proteolysis of RACK1 by Pronase, and protects temperature-dependent degradation of RACK1[1].
Harringtonolide (0-4 μM; 24 hours) suppresses the phosphorylation of FAK dose-dependently and inhibits Src and STAT3, the downstream proteins of FAK[1].
Harringtonolide (0-4 μM; 24 hours) dose-dependently inhibits the RACK1–FAK interaction in A375 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line: A375 melanoma cells[1]
Concentration: 0-50 μM
Incubation Time: 24 hours
Result: Showed good antiproliferation activity with IC50 of 39.66 μM.

Western Blot Analysis

Cell Line: A375 melanoma cells[1]
Concentration: 0, 0.5, 1, 2, 4 μM
Incubation Time: 24 hours
Result: Suppressed the phosphorylation of FAK dose-dependently and inhibited Src and STAT3, the downstream proteins of FAK.
Molecular Weight

310.34

Formula

C19H18O4

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1C=C(CC2)C3=C([C@H]4C(C5O6)C3[C@@]2([C@H](C)[C@H]5O4)C6=O)C(C)=C1

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (16.11 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2223 mL 16.1114 mL 32.2227 mL
5 mM 0.6445 mL 3.2223 mL 6.4445 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation

Purity: ≥98.0%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2223 mL 16.1114 mL 32.2227 mL 80.5568 mL
5 mM 0.6445 mL 3.2223 mL 6.4445 mL 16.1114 mL
10 mM 0.3222 mL 1.6111 mL 3.2223 mL 8.0557 mL
15 mM 0.2148 mL 1.0741 mL 2.1482 mL 5.3705 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Harringtonolide
Cat. No.:
HY-N10335
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