1. GPCR/G Protein
    Neuronal Signaling
  2. mAChR
  3. Ipratropium bromide hydrate

Ipratropium bromide hydrate (Synonyms: Sch 1000 bromide hydrate)

Cat. No.: HY-B1332
Handling Instructions

Ipratropium bromide (Sch 1000) hydrate is a muscarinic receptor antagonist, with IC50s of 2.9 nM, 2 nM, and 1.7 nM for M1, M2, and M3 receptors, respectively. Ipratropium bromide hydrate relaxes smooth muscle, can be used in the research for COPD (chronic obstructive pulmonary disease) and asthma.

For research use only. We do not sell to patients.

Ipratropium bromide hydrate Chemical Structure

Ipratropium bromide hydrate Chemical Structure

CAS No. : 66985-17-9

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Based on 1 publication(s) in Google Scholar

Other Forms of Ipratropium bromide hydrate:

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Description

Ipratropium bromide (Sch 1000) hydrate is a muscarinic receptor antagonist, with IC50s of 2.9 nM, 2 nM, and 1.7 nM for M1, M2, and M3 receptors, respectively. Ipratropium bromide hydrate relaxes smooth muscle, can be used in the research for COPD (chronic obstructive pulmonary disease) and asthma[1][2][3][4][5].

IC50 & Target

muscarinic M1 receptor

2.9 nM (IC50)

muscarinic M2 receptor

2 nM (IC50)

muscarinic M3 receptor

1.7 nM (IC50)

In Vitro

Ipratropium bromide hydrate (1 nM, 10 nM, 100 nM; 15 min) exerts its toxic effects via disruption of mitochondrial membrane potential[1].
Ipratropium bromide hydrate (1 nM-1 μM; 4 h) increases infarct size in isolated perfused heart ischaemia/reperfusion experiments with a dose-responsive manner (EC50=22.7 nM)[1].
Ipratropium bromide hydrate (0.001 nM-0.1 mM; 2 h) inhibits adult rat cardiac myocyte growth after 4 h hypoxia treatment[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Adult Rat Cardiac Myocyte
Concentration: 0.001 nM-0.1 mM
Incubation Time: 2 h in dark; prior to 4 h hypoxia
Result: Resulted cell viability in a dose-dependent manner, with the inhibition rate of 52.7% at 0.1 mM dose.
In Vivo

Ipratropium bromide hydrate (1.0 μg/kg; i.v.; single dose) enhances vagal nerve stimulation induing bronchoconstriction[2].
Ipratropium bromide hydrate (0.04 mg/20 mL and 0.20 mg/20 mL; inhalation for 30 min, rate=30 mL/30 min) can protect the lungs against the cadmium-induced acute neutrophilic inflammation by reducing the parenchyma inflammatory infiltration of neutrophils[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Guinea-pigs of the Dunkin Hartley strain[2]
Dosage: 0.1-1 μg/kg
Administration: Intravenous injection; single dose
Result: Resulted little blocking effect on post-junctional muscarinic receptors at 0.3 μg/kg, and inhibited ACh-induced bronchoconstriction at 0.5 μg/kg.
Animal Model: Male Sprague-Dawley rats (300-350 g)[4]
Dosage: 0.04 mg/20 mL and 0.20 mg/20 mL
Administration: Inhalation; atomization rate of 30 mL/30 min; 30 min
Result: Had no significant effects on any parameters recorded in healthy rats but exerted a protective effect against the inflammatory reaction elicited by cadmium.
Molecular Weight

430.38

Formula

C20H32BrNO4

CAS No.
SMILES

C[N+]1([[email protected]]2CC[[email protected]]1CC(OC(C(CO)C3=CC=CC=C3)=O)C2)C(C)C.O.[Br-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 62.5 mg/mL (145.22 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3235 mL 11.6176 mL 23.2353 mL
5 mM 0.4647 mL 2.3235 mL 4.6471 mL
10 mM 0.2324 mL 1.1618 mL 2.3235 mL
*Please refer to the solubility information to select the appropriate solvent.
Purity & Documentation
References
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Ipratropium bromide hydrate
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HY-B1332
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