JNK3 inhibitor-4
Based on 1 Customer Validation
JNK3 inhibitor-4 is a potent and BBB-permeable inhibitor of JNK3 (IC50=1.0 nM) based on 2-aryl-1-pyrimidinyl-1H-imidazole-5-yl acetonitrile. JNK3 inhibitor-4 shows excellent selectivity over other protein kinases including isoforms JNK1 (IC50=143.9 nM) and JNK2 (IC50=298.2 nM). JNK3 inhibitor-4 has neuroprotective effect and predicated blood-brain barrier permeability.
For research use only. We do not sell to patients.
- Purity: 99.37%
- CAS No.: 2409109-65-3
- Formula: C28H27N7O
- Molecular Weight:477.56
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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JNK3 1.0 nM (IC50) |
JNK1 143.9 nM (IC50) |
JNK2 298.2 nM (IC50) |
JNK3 inhibitor-4 (compound 15d) (1, 5, 10, 20 μM; 24 h and 48 h) inhibits Aβ1-42 induced Aβ1-42-induced cellular toxicity in primary rat cortex neuron[1].
JNK3 inhibitor-4 (10 μM and 20 μM; 24 h and 48 h) inhibits c-jun phosphorylation and APP phosphorylation induced by 10 μM Aβ1-42 or 0.5 μM Anisomycin (HY-18982) in primary rat cortex neuron[1].
JNK3 inhibitor-4 (50 μM; 4 h) shows high permeability in Caco-2 assay and is predicted as BBB permeable (CNS+) based on effective permeability coefficient (Pe) value > 4 in PAMPA assay[1].
JNK3 inhibitor-4 also shows inhibitory potency on GSK3α (h), GSK3β (h), JNK1, 2, MKK6, MOK, SAPK2a (h), SAPK2a (T106 M) (h), SAPK2b (h), MKK4, JNK1α1 (h), and JNK2α1 (h). These shows IC50 values of 5.78, 11.7, 15.1, 1.18, 3.10, 1.19, 0.280, 0.970, 0.860, and 0.340 μM, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Primary rat cortex neuron
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Concentration:10 μM and 20 μM
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Incubation Time:24 hours
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Result:Monomeric Aβ1-42 induced c-jun phosphorylation dose-dependently and that was inhibited by JNK3 inhibitor-4 in concentration-dependent manner.
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Cell Line:Primary rat cortex neuron
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Concentration:1, 5, 10, 20 μM
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Incubation Time:24 hours and 48 hours
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Result:Showed neuroprotective effect and increased cell viability under Aβ1-42 treatment.
JNK3 inhibitor-4 (30 mg/kg; p.o.; single dose) shows blood-brain barrier permeability with brain to plasma ratio of 0.02 in SD rats[1].
Pharmacokinetics in rats[1]
| Route | Dose (mg/kg) | AUC0-t (ng·h/mL) | Cmax (ng/mL) | Tmax (h) | T1/2 (h) | BA (%) |
| IV | 1 | 718.0 | 0.22 | |||
| PO | 3 | 337.5 | 377.82 | 0.63 | 0.34 | 15.67 |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2409109-65-3
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Appearance Solid
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Molecular Weight 477.56
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Formula C28H27N7O
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Color Off-white to light yellow
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SMILES
N#CCC1=CN=C(N1C2=NC(N[C@H]3CCCN(C3)C(C4CC4)=O)=NC=C2)C5=CC6=C(C=C5)C=CC=C6
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (209.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.23 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0940 mL | 10.4699 mL | 20.9398 mL | 52.3494 mL |
| 5 mM | 0.4188 mL | 2.0940 mL | 4.1880 mL | 10.4699 mL | |
| 10 mM | 0.2094 mL | 1.0470 mL | 2.0940 mL | 5.2349 mL | |
| 15 mM | 0.1396 mL | 0.6980 mL | 1.3960 mL | 3.4900 mL | |
| 20 mM | 0.1047 mL | 0.5235 mL | 1.0470 mL | 2.6175 mL | |
| 25 mM | 0.0838 mL | 0.4188 mL | 0.8376 mL | 2.0940 mL | |
| 30 mM | 0.0698 mL | 0.3490 mL | 0.6980 mL | 1.7450 mL | |
| 40 mM | 0.0523 mL | 0.2617 mL | 0.5235 mL | 1.3087 mL | |
| 50 mM | 0.0419 mL | 0.2094 mL | 0.4188 mL | 1.0470 mL | |
| 60 mM | 0.0349 mL | 0.1745 mL | 0.3490 mL | 0.8725 mL | |
| 80 mM | 0.0262 mL | 0.1309 mL | 0.2617 mL | 0.6544 mL | |
| 100 mM | 0.0209 mL | 0.1047 mL | 0.2094 mL | 0.5235 mL |
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