NPPM 6748-481

NPPM 6748-481 (30 min) potently and selectively inhibits purified yeast Sec14-catalyzed [³H]PtdIns transfer in vitro with an IC₅₀ of 211 nM, and does not inhibit related yeast Sfh1-Sfh5 PITPs at concentrations up to 40 μM^[1].
NPPM 6748-481 (10-16 h) inhibits growth of WT haploid Saccharomyces cerevisiae with an IC₅₀ of 2.87 μM, and sensitivity to the compound directly correlates with cellular Sec14 levels^[1].
NPPM 6748-481 (30 min) has drastically reduced inhibitory sensitivity against purified yeast Sec14 with mutations in specific residues (Y111, Y151, S201, S173) in vitro, with Sec14^S173C showing >736-fold resistance^[1].
NPPM 6748-481 (20-40 μM; 48 h) does not inhibit growth of Saccharomyces cerevisiae expressing NPPM-resistant Sec14^S173C, and "bypass Sec14" cki1Δ and kes1Δ mutants are also resistant to the compound, confirming Sec14 as the sole essential cellular target^[1].
NPPM 6748-481 (20 μM; 3 h) selectively disrupts TGN-endosomal PtdIns-4-P pools in WT Saccharomyces cerevisiae without affecting plasma membrane PtdIns-4-P pools, phenocopying genetic Sec14 inactivation^[1].
NPPM 6748-481 (20 μM) does not interfere with Sfh4-dependent PtdSer decarboxylation to PtdEtn in Saccharomyces cerevisiae psd1Δ mutants, demonstrating selective inhibition of Sec14-dependent versus Sfh4-dependent PtdIns-4-P signaling in TGN-endosomes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.