MRC71 

MRC71 is a TRIMTAC degrader that recruits endogenous TRIM21 E3 ligase at one end and covalently binds to HaloTag-fusion target proteins at the other end. MRC71 selectively degrades oligomeric CAV1- and Cavin1-mEGFP-Halo, but not monomeric mEGFP-Halo, and exhibits a typical concentration-dependent degradation profile with a hook effect. MRC71 can be used to verify TRIMTAC ternary complex formation and state-selective degradation in cells^[1].

MRC71 (2.5-20 μM) drives the formation of a ternary complex between purified TRIM21 PRYSPRY and HaloTag protein in vitro, with a characteristic hook effect occurring at excessively high compound concentrations^[1].
MRC71 (0.001-100 μM; 2 h) induces the formation of a dose-dependent ternary complex between full-length TRIM21 and H₂B-mEGFP-Halo in RPE-1 cells, with a hook effect occurring at excessively high compound concentrations^[1].
MRC71 (0.01-100 μM; 48 h) is a TRIM21-dependent TRIMTAC that selectively degrades oligomerized CAV1-mEGFP-Halo and Cavin1-mEGFP-Halo in RPE-1 cells, but does not affect monomeric mEGFP-Halo^[1].
MRC71 (2.5 μM; 24 h) selectively degrades membrane-associated oligomeric Cavin1-mEGFP-Halo via the proteasome in RPE-1 cells, while preserving the cytoplasmic monomer pool and smaller Cavin1-free cleavage products^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

816.47

C₄₃H₆₆ClN₅O₈

3070832-68-4

O=C(C[C@H](N)C1=C(OC)C=CC=C1)N(C2)[C@H](C(N[C@@H](CC3=CC=NC=C3)C(NCCOCCOCCOCCOCCCCCCCl)=O)=O)C[C@H]2C4CCCCC4

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Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Luptak J, Clift D, Mukadam A, et al.. State-selective small molecule degraders that preferentially remove aggregates and oligomers. Nature communications. 2025 Nov 25;16(1):10486.  [Content Brief]