Art. -Nr.: HY-118620

Nortriptyline

(Synonyms: Desmethylamitriptyline; Desitriptilina)

5 Cited Publications

Kundenbewertung

Based on 5 publication(s) in Google Scholar

Nortriptyline (Desmethylamitriptyline), the main active metabolite of Amitriptyline, is a tricyclic antidepressant. Nortriptyline is a potent autophagy inhibitor and has anticancer effects. N

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Reinheit: 99.94%
CAS. Nr.: 72-69-5
Formel: C₁₉H₂₁N
Molecular Weight:263.38

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Nortriptyline Chemical Structure

CAS. Nr.: 72-69-5

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (5)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Nortriptyline (Desmethylamitriptyline), the main active metabolite of Amitriptyline, is a tricyclic antidepressant. Nortriptyline is a potent autophagy inhibitor and has anticancer effects^[1]^[2]^[3]. N

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	171 μM Compound: 9c	Inhibition of mouse mGAT2 expressed in HEK293 cells assessed as inhibition of [3H]GABA uptake after 3 mins incubation by TopCount microplate scintillation counting analysis Inhibition of mouse mGAT2 expressed in HEK293 cells assessed as inhibition of [3H]GABA uptake after 3 mins incubation by TopCount microplate scintillation counting analysis	[PMID: 25882526]
HEK293	IC₅₀	249 μM Compound: 9c	Inhibition of mouse mGAT4 expressed in HEK293 cells assessed as inhibition of [3H]GABA uptake after 3 mins incubation by TopCount microplate scintillation counting analysis Inhibition of mouse mGAT4 expressed in HEK293 cells assessed as inhibition of [3H]GABA uptake after 3 mins incubation by TopCount microplate scintillation counting analysis	[PMID: 25882526]
HEK293	IC₅₀	270 μM Compound: 9c	Inhibition of mouse mGAT3 expressed in HEK293 cells assessed as inhibition of [3H]GABA uptake after 3 mins incubation by TopCount microplate scintillation counting analysis Inhibition of mouse mGAT3 expressed in HEK293 cells assessed as inhibition of [3H]GABA uptake after 3 mins incubation by TopCount microplate scintillation counting analysis	[PMID: 25882526]
HEK293	IC₅₀	389 μM Compound: 9c	Inhibition of mouse mGAT1 expressed in HEK293 cells assessed as inhibition of [3H]GABA uptake after 3 mins incubation by TopCount microplate scintillation counting analysis Inhibition of mouse mGAT1 expressed in HEK293 cells assessed as inhibition of [3H]GABA uptake after 3 mins incubation by TopCount microplate scintillation counting analysis	[PMID: 25882526]

In Vitro

Amitriptyline is metabolized by CYP2C19 to the active metabolite, Nortriptyline. Nortriptyline blocks the reuptake of Norepinephrine more potently than Serotonin^[1].
Nortriptyline (6.25-100 μM; 24-72 h) markedly reducs the viability of TCCSUP and mouse MBT-2 bladder cancer cells in a concentration- and time-dependent manner^[3].
Nortriptyline (12.55-100 μM; 24 h) induces cell cycle arrest and apoptosis in TCCSUP and MBT-2 cells^[3].
Nortriptyline (12.55-100 μM; 24 h) induces both intrinsic and extrinsic apoptosis in these bladder cancer cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:

Human TCCSUP and mouse MBT-2 bladder cancer cells
Concentration:

6.25 μM, 12.5 μM, 25 μM, 50 μM and 100 μM
Incubation Time:

24, 48, or 72 h
Result:

Exhibited cytotoxic effects on TCCSUP and MBT-2 cells.

Cell Cycle Analysis^[3]

Cell Line:

Human TCCSUP and mouse MBT-2 bladder cancer cells
Concentration:

25 μM, 50 μM, or 100 μM (TCCSUP); 12.5 μM, 25 μM, or 50 μM (MBT-2 cells)
Incubation Time:

24 h
Result:

Caused cell cycle arrest in these bladder cancer cells.

Apoptosis Analysis^[3]

Cell Line:

Human TCCSUP and mouse MBT-2 bladder cancer cells
Concentration:

25 μM, 50 μM, or 100 μM (TCCSUP); 12.5 μM, 25 μM, or 50 μM (MBT-2 cells)
Incubation Time:

24 h
Result:

Induced apoptosis in both TCCSUP and MBT-2 cells.

Western Blot Analysis^[3]

Cell Line:

Human TCCSUP and mouse MBT-2 bladder cancer cells
Concentration:

25 μM, 50 μM, or 100 μM (TCCSUP); 12.5 μM, 25 μM, or 50 μM (MBT-2 cells)
Incubation Time:

24 h
Result:

Increased the expression of Fas, FasL, FADD, Bax, Bak, and cleaved forms of caspase-3, caspase-8, caspase-9, and poly(ADP-ribose) polymerase. Decreased the expression of Bcl-2, Bcl-xL, BH3 interacting domain death agonist, X-linked inhibitor of apoptosis protein, and survivin.

In Vivo

Nortriptyline (10-20 mg/kg; ip; every day; for three weeks) inhibits the growth of bladder tumors in mice inoculated with MBT-2 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Adult male C3H/HeN mice (25-30 g; 2-3 months of age) injected with MBT-2 cells^[3]
Dosage:

10 or 20 mg/kg
Administration:

Intraperitoneal injection; every day; for three weeks.
Result:

Suppressed tumor growth in mice inoculated with MBT-2 cells.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS. Nr. 72-69-5
Appearance Solid
Molecular Weight 263.38
Formel C₁₉H₂₁N
Color White to off-white
SMILES

CNCC/C=C1C2=CC=CC=C2CCC3=CC=CC=C\13
Synonyms

Desmethylamitriptyline; Desitriptilina
Structure Classification
- Alkaloids
- Other Alkaloids
Initial Source
- Plants
- Labiatae
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (5)

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Journal Impact Factor

Cell Commun Signal

Tricyclic antidepressants induce liver inflammation by targeting NLRP3 inflammasome activation. [Abstract]

2023 May 25;21(1):123. PMID: 37231437
J Exp Med

Repurposing a tricyclic antidepressant in tumor and metabolism disease treatment through fatty acid uptake inhibition. [Abstract]

2023 Mar 6;220(3):e20221316. PMID: 36520461
Int J Biol Macromol

RNA binding motif single-stranded interacting protein 1 drives renal fibrosis by stabilizing Annexin A8 mRNA to activate PI3K/AKT signaling. [Abstract]

2026 May 24:368:152687. PMID: 42184845
EMBO Mol Med

RBMS1 orchestrates cardiac hypertrophy by facilitating CTTN splice-switching and sarcomere dynamics. [Abstract]

2025 Dec;17(12):3555-3585. PMID: 41214391
Pharmaceuticals (Basel)

Comparative Pharmacological Profiling of Psychotherapeutic Drugs Reveals a Functional Taxonomy Based on Direct Inhibition of Smooth Muscle Excitability. [Abstract]

2026 Apr 21;19(4):645. PMID: 42075900

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : ≥ 100 mg/mL (379.68 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.7968 mL

18.9840 mL

37.9680 mL

5 mM

0.7594 mL

3.7968 mL

7.5936 mL

10 mM

0.3797 mL

1.8984 mL

3.7968 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (9.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (9.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (9.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (284 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Dean L. Amitriptyline Therapy and CYP2D6 and CYP2C19 Genotype. In: Pratt VM, Scott SA, Pirmohamed M, et al., eds. Medical Genetics Summaries. Bethesda (MD): National Center for Biotechnology Information (US); March 23, 2017. [Content Brief]

[2]. Petrosyan E, et al. Repurposing Autophagy Regulators in Brain Tumors [published online ahead of print, 2022 Feb 18]. Int J Cancer. 2022;10.1002/ijc.33965. [Content Brief]

[3]. Sheau-Yun Yuan, et al. Nortriptyline induces mitochondria and death receptor-mediated apoptosis in bladder cancer cells and inhibits bladder tumor growth in vivo. Eur J Pharmacol. 2015 Aug 15:761:309-20. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.7968 mL	18.9840 mL	37.9680 mL	94.9199 mL
	5 mM	0.7594 mL	3.7968 mL	7.5936 mL	18.9840 mL
	10 mM	0.3797 mL	1.8984 mL	3.7968 mL	9.4920 mL
	15 mM	0.2531 mL	1.2656 mL	2.5312 mL	6.3280 mL
	20 mM	0.1898 mL	0.9492 mL	1.8984 mL	4.7460 mL
	25 mM	0.1519 mL	0.7594 mL	1.5187 mL	3.7968 mL
	30 mM	0.1266 mL	0.6328 mL	1.2656 mL	3.1640 mL
	40 mM	0.0949 mL	0.4746 mL	0.9492 mL	2.3730 mL
	50 mM	0.0759 mL	0.3797 mL	0.7594 mL	1.8984 mL
	60 mM	0.0633 mL	0.3164 mL	0.6328 mL	1.5820 mL
	80 mM	0.0475 mL	0.2373 mL	0.4746 mL	1.1865 mL
	100 mM	0.0380 mL	0.1898 mL	0.3797 mL	0.9492 mL

Keywords

Nortriptyline

5 Cited Publications

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Publications Citing Use of MedChemExpress (MCE) Nortriptyline

Biologische Aktivität

Chemical Information

Publications (5)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Nortriptyline Related Classifications

HY-118620 Related Classifications