2. Autophagy Metabolic Enzyme/Protease Apoptosis
  3. Autophagy Drug Metabolite Apoptosis
  4. Nortriptyline

Nortriptyline  (Synonyms: Desmethylamitriptyline; Desitriptilina)

Cat. No.: HY-118620
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Nortriptyline (Desmethylamitriptyline), the main active metabolite of Amitriptyline, is a tricyclic antidepressant. Nortriptyline is a potent autophagy inhibitor and has anticancer effects. N

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Nortriptyline Chemical Structure

Nortriptyline Chemical Structure

CAS No. : 72-69-5

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Based on 2 publication(s) in Google Scholar

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Nortriptyline Related Antibodies

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Description

Nortriptyline (Desmethylamitriptyline), the main active metabolite of Amitriptyline, is a tricyclic antidepressant. Nortriptyline is a potent autophagy inhibitor and has anticancer effects[1][2][3]. N

In Vitro

Amitriptyline is metabolized by CYP2C19 to the active metabolite, Nortriptyline. Nortriptyline blocks the reuptake of Norepinephrine more potently than Serotonin[1].
Nortriptyline (6.25-100 μM; 24-72 h) markedly reducs the viability of TCCSUP and mouse MBT-2 bladder cancer cells in a concentration- and time-dependent manner[3].
Nortriptyline (12.55-100 μM; 24 h) induces cell cycle arrest and apoptosis in TCCSUP and MBT-2 cells[3].
Nortriptyline (12.55-100 μM; 24 h) induces both intrinsic and extrinsic apoptosis in these bladder cancer cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Nortriptyline Related Antibodies

Cell Viability Assay[3]

Cell Line: Human TCCSUP and mouse MBT-2 bladder cancer cells
Concentration: 6.25 μM, 12.5 μM, 25 μM, 50 μM and 100 μM
Incubation Time: 24, 48, or 72 h
Result: Exhibited cytotoxic effects on TCCSUP and MBT-2 cells.

Cell Cycle Analysis[3]

Cell Line: Human TCCSUP and mouse MBT-2 bladder cancer cells
Concentration: 25 μM, 50 μM, or 100 μM (TCCSUP); 12.5 μM, 25 μM, or 50 μM (MBT-2 cells)
Incubation Time: 24 h
Result: Caused cell cycle arrest in these bladder cancer cells.

Apoptosis Analysis[3]

Cell Line: Human TCCSUP and mouse MBT-2 bladder cancer cells
Concentration: 25 μM, 50 μM, or 100 μM (TCCSUP); 12.5 μM, 25 μM, or 50 μM (MBT-2 cells)
Incubation Time: 24 h
Result: Induced apoptosis in both TCCSUP and MBT-2 cells.

Western Blot Analysis[3]

Cell Line: Human TCCSUP and mouse MBT-2 bladder cancer cells
Concentration: 25 μM, 50 μM, or 100 μM (TCCSUP); 12.5 μM, 25 μM, or 50 μM (MBT-2 cells)
Incubation Time: 24 h
Result: Increased the expression of Fas, FasL, FADD, Bax, Bak, and cleaved forms of caspase-3, caspase-8, caspase-9, and poly(ADP-ribose) polymerase. Decreased the expression of Bcl-2, Bcl-xL, BH3 interacting domain death agonist, X-linked inhibitor of apoptosis protein, and survivin.
In Vivo

Nortriptyline (10-20 mg/kg; ip; every day; for three weeks) inhibits the growth of bladder tumors in mice inoculated with MBT-2 cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adult male C3H/HeN mice (25-30 g; 2-3 months of age) injected with MBT-2 cells[3]
Dosage: 10 or 20 mg/kg
Administration: Intraperitoneal injection; every day; for three weeks.
Result: Suppressed tumor growth in mice inoculated with MBT-2 cells.
Clinical Trial
Molecular Weight

263.38

Formula

C19H21N
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CNCC/C=C1C2=CC=CC=C2CCC3=CC=CC=C\13
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation

Purity: 99.64%

COA (255 KB) HNMR (118 KB) RP-HPLC (187 KB)

Handling Instructions (2659 KB)
References
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Nortriptyline Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nortriptyline72-69-5Desmethylamitriptyline DesitriptilinaAutophagyDrug MetaboliteApoptosisdepressionmetabolitetricyclic antidepressanttumorInhibitorinhibitorinhibit

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