Surface plasmon resonance approach to study drug interactions with SARS-CoV-2 RNA-dependent RNA polymerase highlights treatment potential of suramin

  • J Virol Methods. 2021 Dec;298:114283. doi: 10.1016/j.jviromet.2021.114283.
Martina Mravinec  1 ,  Gregor Bajc  1 ,  Matej Butala  2
Affiliations
  • 1. Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana 1000, Slovenia.
  • 2. Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana 1000, Slovenia. Electronic address: [email protected].
PMID: 34534610 DOI: 10.1016/j.jviromet.2021.114283
Abstract

The SARS-CoV-2 RNA-dependent RNA polymerase (RdRp) is essential for virus replication, therefore it is a promising drug target. Here we present a surface plasmon resonance approach to study the interaction of RdRp with drugs in real time. We monitored the effect of favipiravir, ribavirin, sofosbuvir triphosphate PSI-7409 and suramin on RdRp binding to RNA immobilized on the chip. Suramin precluded interaction of RdRp with RNA and even displaced RdRp from RNA.

Keywords
RNA-dependent RNA polymerase; SARS-CoV-2; Suramin; Surface plasmon resonance.
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