1. Anti-infection
  2. HCV
  3. PSI-7409


Cat. No.: HY-15745
COA Handling Instructions

PSI-7409 is the active 5'-triphosphate metabolite of Sofosbuvir (PSI-7977). Sofosbuvir (PSI-7977) is a selective and highly active nucleotide analog inhibitor of HCV.

The free form of the compound is prone to instability, it is advisable to consider the stable salt form (PSI-7409 tetrasodium) that retains the same biological activity.

For research use only. We do not sell to patients.

PSI-7409 Chemical Structure

PSI-7409 Chemical Structure

CAS No. : 1015073-42-3

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PSI-7409 is the active 5'-triphosphate metabolite of Sofosbuvir (PSI-7977). Sofosbuvir (PSI-7977) is a selective and highly active nucleotide analog inhibitor of HCV.

In Vitro

PSI-7409 inhibits the enzymatic activities of these NS5BΔ21 polymerases in a dose-dependent manner. The IC50s for PSI-7409 against GT 1b, 2a, 3a, and 4a NS5B polymerases are 1.6 μM, 2.8 μM, 0.7 μM, and 2.6 μM, respectively. PSI-7409 is a weak inhibitor of DNA Pol α (IC50=550 μM). DNA Pol β and γ are not inhibited by 1 mM PSI-7409. A significant amount of RNA product is made in the presence of 500 μM PSI-7409, about 85%[1]. In clone A cells, the levels of PSI-7409 gradually increases to a maximum concentration of about 25 μm over a period of 48 h. PSI-7409 forms at a much faster rate in primary human hepatocytes, achieving a maximum intracellular concentration of ∼100 μM at 4 h and remains at that concentration for 48 h[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight







White to off-white




Room temperature in continental US; may vary elsewhere.

Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (99.97 mM; Need ultrasonic)

DMF : < 1 mg/mL (insoluble)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9994 mL 9.9968 mL 19.9936 mL
5 mM 0.3999 mL 1.9994 mL 3.9987 mL
10 mM 0.1999 mL 0.9997 mL 1.9994 mL
*Please refer to the solubility information to select the appropriate solvent.
Purity & Documentation
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PSI-7409 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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This equation is commonly abbreviated as: C1V1 = C2V2

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