PSI-7409
Based on 8 publication(s) in Google Scholar
PSI-7409 is the active 5'-triphosphate metabolite of Sofosbuvir (PSI-7977). Sofosbuvir (PSI-7977) is a selective and highly active nucleotide analog inhibitor of HCV.
For research use only. We do not sell to patients.
- CAS No.: 1015073-42-3
- Formula: C10H16FN2O14P3
- Molecular Weight:500.16
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) PSI-7409
More- Cell. 2022 Nov 10;185(23):4347-4360.e17. [Abstract]
- Nat Commun. 2026 Jan 13;17(1):1601. [Abstract]
- Asian J Pharm Sci. 2021 Nov;16(6):772-783. [Abstract]
- J Med Chem. 2025 Jun 26;68(12):12414-12433. [Abstract]
- Antiviral Res. 2020 Mar:175:104708. [Abstract]
- Microbiol Spectr. 2022 Oct 26;10(5):e0272922. [Abstract]
- Biochem Biophys Res Commun. 2023 Jan 22:641:50-56. [Abstract]
- J Virol Methods. 2021 Dec:298:114283. [Abstract]
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HeLa | IC50 |
>100 μM
Compound: 2'-F,2'-C-Me-UTP
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Inhibition of RNA polymerase 2 isolated from human HeLa cell nuclear extract assessed as decrease in incorporation of P32-alpha-CTP after 2 hrs by scintillation counting method
Inhibition of RNA polymerase 2 isolated from human HeLa cell nuclear extract assessed as decrease in incorporation of P32-alpha-CTP after 2 hrs by scintillation counting method
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[PMID: 28595015] |
| HeLa | IC50 |
>100 μM
Compound: 3a-TP
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Inhibition of RNA polymerase 2 in human HeLa cell nuclear extract assessed as reduction in RNA transcription by PAGE analysis
Inhibition of RNA polymerase 2 in human HeLa cell nuclear extract assessed as reduction in RNA transcription by PAGE analysis
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[PMID: 30951311] |
In Vitro
PSI-7409 inhibits the enzymatic activities of these NS5BΔ21 polymerases in a dose-dependent manner. The IC50s for PSI-7409 against GT 1b, 2a, 3a, and 4a NS5B polymerases are 1.6 μM, 2.8 μM, 0.7 μM, and 2.6 μM, respectively. PSI-7409 is a weak inhibitor of DNA Pol α (IC50=550 μM). DNA Pol β and γ are not inhibited by 1 mM PSI-7409. A significant amount of RNA product is made in the presence of 500 μM PSI-7409, about 85%[1]. In clone A cells, the levels of PSI-7409 gradually increases to a maximum concentration of about 25 μm over a period of 48 h. PSI-7409 forms at a much faster rate in primary human hepatocytes, achieving a maximum intracellular concentration of ～100 μM at 4 h and remains at that concentration for 48 h[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1015073-42-3
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Appearance Solid
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Molecular Weight 500.16
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Formula C10H16FN2O14P3
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Color White to off-white
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SMILES
O[C@@H]([C@@](C)(F)[C@H](N1C(NC(C=C1)=O)=O)O2)[C@H]2COP(O)(OP(OP(O)(O)=O)(O)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (8)
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Journal Impact Factor
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Most Recent
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Cell
A mechanism for SARS-CoV-2 RNA capping and its inhibition by nucleotide analog inhibitors. [Abstract]2022 Nov 10;185(23):4347-4360.e17. PMID: 36335936 -
Nat Commun
2026 Jan 13;17(1):1601. PMID: 41530153 -
Asian J Pharm Sci
Liposomal remdesivir inhalation solution for targeted lung delivery as a novel therapeutic approach for COVID-19. [Abstract]2021 Nov;16(6):772-783. PMID: 34703490 -
J Med Chem
Identification of 4'-Thiouridine as an Orally Available Antiviral Agent Targeting Both RdRp and NiRAN Functions of SARS-CoV-2 Nsp12. [Abstract]2025 Jun 26;68(12):12414-12433. PMID: 40512093 -
Antiviral Res
Evaluation of sofosbuvir activity and resistance profile against West Nile virus in vitro. [Abstract]2020 Mar:175:104708. PMID: 31931104 -
Microbiol Spectr
RNA-Dependent RNA Polymerase of the Second Human Pegivirus Exhibits a High-Fidelity Feature. [Abstract]2022 Oct 26;10(5):e0272922. PMID: 35980196 -
Biochem Biophys Res Commun
Sofosbuvir and its tri-phosphate metabolite inhibit the RNA-dependent RNA polymerase activity of non-structural protein 5 from the Kyasanur forest disease virus. [Abstract]2023 Jan 22:641:50-56. PMID: 36521285 -
J Virol Methods
Surface plasmon resonance approach to study drug interactions with SARS-CoV-2 RNA-dependent RNA polymerase highlights treatment potential of suramin. [Abstract]2021 Dec:298:114283. PMID: 34534610
Solvent & Solubility
In Vitro:
H2O : 50 mg/mL (99.97 mM; Need ultrasonic)
DMF : < 1 mg/mL (insoluble)
DMSO : < 1 mg/mL (insoluble or slightly soluble)
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Lam AM, et al. PSI-7851, a pronucleotide of beta-D-2'-deoxy-2'-fluoro-2'-C-methyluridine monophosphate, is a potent and pan-genotype inhibitor of hepatitis C virus replication. Antimicrob Agents Chemother. 2010 Aug;54(8):3187-96. [Content Brief]
[2]. Murakami E, et al. Mechanism of activation of PSI-7851 and its diastereoisomer PSI-7977. [Content Brief]
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.9994 mL | 9.9968 mL | 19.9936 mL | 49.9840 mL |
| 5 mM | 0.3999 mL | 1.9994 mL | 3.9987 mL | 9.9968 mL | |
| 10 mM | 0.1999 mL | 0.9997 mL | 1.9994 mL | 4.9984 mL | |
| 15 mM | 0.1333 mL | 0.6665 mL | 1.3329 mL | 3.3323 mL | |
| 20 mM | 0.1000 mL | 0.4998 mL | 0.9997 mL | 2.4992 mL | |
| 25 mM | 0.0800 mL | 0.3999 mL | 0.7997 mL | 1.9994 mL | |
| 30 mM | 0.0666 mL | 0.3332 mL | 0.6665 mL | 1.6661 mL | |
| 40 mM | 0.0500 mL | 0.2499 mL | 0.4998 mL | 1.2496 mL | |
| 50 mM | 0.0400 mL | 0.1999 mL | 0.3999 mL | 0.9997 mL | |
| 60 mM | 0.0333 mL | 0.1666 mL | 0.3332 mL | 0.8331 mL | |
| 80 mM | 0.0250 mL | 0.1250 mL | 0.2499 mL | 0.6248 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
PSI-7409 Related Classifications
HY-15745 Related Classifications
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