PSI-7409
Based on 8 publication(s) in Google Scholar
PSI-7409 is the active 5'-triphosphate metabolite of Sofosbuvir (PSI-7977). Sofosbuvir (PSI-7977) is a selective and highly active nucleotide analog inhibitor of HCV.
For research use only. We do not sell to patients.
- CAS No.: 1015073-42-3
- Formula: C10H16FN2O14P3
- Molecular Weight:500.16
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) PSI-7409
More- Cell. 2022 Nov 10;185(23):4347-4360.e17. [Abstract]
- Nat Commun. 2026 Jan 13;17(1):1601. [Abstract]
- Asian J Pharm Sci. 2021 Nov;16(6):772-783. [Abstract]
- J Med Chem. 2025 Jun 26;68(12):12414-12433. [Abstract]
- Antiviral Res. 2020 Mar;175:104708. [Abstract]
- Microbiol Spectr. 2022 Oct 26;10(5):e0272922. [Abstract]
- Biochem Biophys Res Commun. 2023 Jan 22:641:50-56. [Abstract]
- J Virol Methods. 2021 Dec:298:114283. [Abstract]
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HeLa | IC50 |
>100 μM
Compound: 2'-F,2'-C-Me-UTP
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Inhibition of RNA polymerase 2 isolated from human HeLa cell nuclear extract assessed as decrease in incorporation of P32-alpha-CTP after 2 hrs by scintillation counting method
Inhibition of RNA polymerase 2 isolated from human HeLa cell nuclear extract assessed as decrease in incorporation of P32-alpha-CTP after 2 hrs by scintillation counting method
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[PMID: 28595015] |
| HeLa | IC50 |
>100 μM
Compound: 3a-TP
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Inhibition of RNA polymerase 2 in human HeLa cell nuclear extract assessed as reduction in RNA transcription by PAGE analysis
Inhibition of RNA polymerase 2 in human HeLa cell nuclear extract assessed as reduction in RNA transcription by PAGE analysis
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[PMID: 30951311] |
PSI-7409 inhibits the enzymatic activities of these NS5BΔ21 polymerases in a dose-dependent manner. The IC50s for PSI-7409 against GT 1b, 2a, 3a, and 4a NS5B polymerases are 1.6 μM, 2.8 μM, 0.7 μM, and 2.6 μM, respectively. PSI-7409 is a weak inhibitor of DNA Pol α (IC50=550 μM). DNA Pol β and γ are not inhibited by 1 mM PSI-7409. A significant amount of RNA product is made in the presence of 500 μM PSI-7409, about 85%[1]. In clone A cells, the levels of PSI-7409 gradually increases to a maximum concentration of about 25 μm over a period of 48 h. PSI-7409 forms at a much faster rate in primary human hepatocytes, achieving a maximum intracellular concentration of ～100 μM at 4 h and remains at that concentration for 48 h[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1015073-42-3
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Appearance Solid
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Molecular Weight 500.16
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Formula C10H16FN2O14P3
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Color White to off-white
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SMILES
O[C@@H]([C@@](C)(F)[C@H](N1C(NC(C=C1)=O)=O)O2)[C@H]2COP(O)(OP(OP(O)(O)=O)(O)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (8)
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Journal Impact Factor
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Most Recent
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Cell
A mechanism for SARS-CoV-2 RNA capping and its inhibition by nucleotide analog inhibitors. [Abstract]2022 Nov 10;185(23):4347-4360.e17. PMID: 36335936 -
Nat Commun
2026 Jan 13;17(1):1601. PMID: 41530153 -
Asian J Pharm Sci
Liposomal remdesivir inhalation solution for targeted lung delivery as a novel therapeutic approach for COVID-19. [Abstract]2021 Nov;16(6):772-783. PMID: 34703490 -
J Med Chem
Identification of 4'-Thiouridine as an Orally Available Antiviral Agent Targeting Both RdRp and NiRAN Functions of SARS-CoV-2 Nsp12. [Abstract]2025 Jun 26;68(12):12414-12433. PMID: 40512093 -
Antiviral Res
Evaluation of sofosbuvir activity and resistance profile against West Nile virus in vitro. [Abstract]2020 Mar;175:104708. PMID: 31931104 -
Microbiol Spectr
RNA-Dependent RNA Polymerase of the Second Human Pegivirus Exhibits a High-Fidelity Feature. [Abstract]2022 Oct 26;10(5):e0272922. PMID: 35980196 -
Biochem Biophys Res Commun
Sofosbuvir and its tri-phosphate metabolite inhibit the RNA-dependent RNA polymerase activity of non-structural protein 5 from the Kyasanur forest disease virus. [Abstract]2023 Jan 22:641:50-56. PMID: 36521285 -
J Virol Methods
Surface plasmon resonance approach to study drug interactions with SARS-CoV-2 RNA-dependent RNA polymerase highlights treatment potential of suramin. [Abstract]2021 Dec:298:114283. PMID: 34534610
Solvent & Solubility
H2O : 50 mg/mL (99.97 mM; Need ultrasonic)
DMF : < 1 mg/mL (insoluble)
DMSO : < 1 mg/mL (insoluble or slightly soluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Lam AM, et al. PSI-7851, a pronucleotide of beta-D-2'-deoxy-2'-fluoro-2'-C-methyluridine monophosphate, is a potent and pan-genotype inhibitor of hepatitis C virus replication. Antimicrob Agents Chemother. 2010 Aug;54(8):3187-96. [Content Brief]
[2]. Murakami E, et al. Mechanism of activation of PSI-7851 and its diastereoisomer PSI-7977. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.9994 mL | 9.9968 mL | 19.9936 mL | 49.9840 mL |
| 5 mM | 0.3999 mL | 1.9994 mL | 3.9987 mL | 9.9968 mL | |
| 10 mM | 0.1999 mL | 0.9997 mL | 1.9994 mL | 4.9984 mL | |
| 15 mM | 0.1333 mL | 0.6665 mL | 1.3329 mL | 3.3323 mL | |
| 20 mM | 0.1000 mL | 0.4998 mL | 0.9997 mL | 2.4992 mL | |
| 25 mM | 0.0800 mL | 0.3999 mL | 0.7997 mL | 1.9994 mL | |
| 30 mM | 0.0666 mL | 0.3332 mL | 0.6665 mL | 1.6661 mL | |
| 40 mM | 0.0500 mL | 0.2499 mL | 0.4998 mL | 1.2496 mL | |
| 50 mM | 0.0400 mL | 0.1999 mL | 0.3999 mL | 0.9997 mL | |
| 60 mM | 0.0333 mL | 0.1666 mL | 0.3332 mL | 0.8331 mL | |
| 80 mM | 0.0250 mL | 0.1250 mL | 0.2499 mL | 0.6248 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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