(R)-3-Hydroxybutanoic acid sodium (Synonyms: (R)-(-)-3-Hydroxybutanoic acid sodium; (R)-3-Hydroxybutyric acid sodium)

Name: (R)-3-Hydroxybutanoic acid sodium
Brand: MedChemExpress
SKU: HY-W015851
Rating: 5.0 (6 reviews)

Cat. No.: HY-W015851 Purity: 99.90%: Data Sheet
COA

SDS
Handling Instructions
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(R)-3-Hydroxybutanoic acid ((R)-3-Hydroxybutyric acid) sodium is a metabolite converted from acetoacetic acid catalyzed by 3-hydroxybutyrate dehydrogenase. (R)-3-Hydroxybutanoic acid sodium can function as a nutrition source, and as a precursor for vitamins, antibiotics and pheromones.

For research use only. We do not sell to patients.

CAS No. : 13613-65-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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Based on 6 publication(s) in Google Scholar

Other Forms of (R)-3-Hydroxybutanoic acid sodium:

(R)-3-Hydroxybutanoic acid-¹³C₂ sodium In-stock
(R)-3-Hydroxybutanoic acid-¹³C₄ sodium In-stock
(R)-3-Hydroxybutanoic acid In-stock
3-Hydroxybutanoic acid-¹³C sodium Get quote
(R)-3-Hydroxybutanoic acid sodium (Standard) Get quote

6 Publications Citing Use of MCE (R)-3-Hydroxybutanoic acid sodium

Bio/Physico-chemical Assay

RT-PCR

Cell Imaging/Staining

ELISA

Histological Imaging/Staining

: (R)-3-Hydroxybutanoic acid sodium purchased from MedChemExpress. Usage Cited in: Sci Adv. 2024 May 31;10(22):eadk5011. [Abstract]; (R)-3-Hydroxybutanoic acid (3-HB) (0.5 mM; 6-24 h) significantly up-regulated the mRNA levels of Gpld1 in HepG2 cells.

: (R)-3-Hydroxybutanoic acid sodium purchased from MedChemExpress. Usage Cited in: Sci Adv. 2024 May 31;10(22):eadk5011. [Abstract]; (R)-3-Hydroxybutanoic acid (3-HB) (0.5 mM; 6-24 h) significantly up-regulated the protein level of Gpld1 in HepG2 cells.

: (R)-3-Hydroxybutanoic acid sodium purchased from MedChemExpress. Usage Cited in: Sci Adv. 2024 May 31;10(22):eadk5011. [Abstract]; Fluorescence microscopy of VSV-GFP viruses in HepG2 cells treated with PBS (Ctrl) or (R)-3-Hydroxybutanoic acid (3-HB) (5 mM) for 24 hours and then infected with VSV-GFP (MOI = 1.0; 24 hours). Scale bars, 200 μm.

: (R)-3-Hydroxybutanoic acid sodium purchased from MedChemExpress. Usage Cited in: Sci Adv. 2024 May 31;10(22):eadk5011. [Abstract]; ELISA analysis of mIFN-β protein levels in the serum of mice administrated with (R)-3-Hydroxybutanoic acid (3-HB) (3 mmol/kg, i.v.) or PBS (Ctrl) for 24 hours. The results showed that 3-HB administration up-regulated serum IFN-I levels.

: (R)-3-Hydroxybutanoic acid sodium purchased from MedChemExpress. Usage Cited in: Sci Adv. 2024 May 31;10(22):eadk5011. [Abstract]; HE staining of the livers from Gpld1^+/+ and Gpld1−/− mice was performed. Gpld1^+/+ and Gpld1−/− mice were administrated with (R)-3-Hydroxybutanoic acid (3-HB) (3 mmol/kg; i.v.) or PBS for 24 hours, and then infected with VSV (1 × 10⁸ PFU/g of body weight; intranasal) for 48 hours. Scale bar: 100 μm.

: (R)-3-Hydroxybutanoic acid sodium purchased from MedChemExpress. Usage Cited in: Am J Physiol Cell Physiol. 2024 Jun 1;326(6):C1710-C1720. [Abstract]; Exposure to 3 mM (R)-3-Hydroxybutanoic acid sodium (d-βOHB, 3 h) increased basal glucose uptake, significantly higher than in the glucose, substrate-free, and 3 mM 1-βOHB groups.

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Description

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

In Vitro

(R)-3-Hydroxybutanoic acid sodium is a metabolite converted from acetoacetic acid. Enhanced hepatic fatty acid oxidation results in the increased production of acetoacetic acid which is in turn converted to (R)-3-Hydroxybutanoic acid sodium by a reaction catalyzed by 3-hydroxybutyrate dehydrogenase^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

126.09

Formula

C₄H₇NaO₃

CAS No.

13613-65-5

Appearance

Solid

Color

White to off-white

SMILES

C[C@H](CC(O[Na])=O)O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (793.08 mM; Need ultrasonic)

DMSO : 50 mg/mL (396.54 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	7.9308 mL	39.6542 mL	79.3084 mL
5 mM	1.5862 mL	7.9308 mL	15.8617 mL
10 mM	0.7931 mL	3.9654 mL	7.9308 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (19.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (19.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (19.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (793.08 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (248 KB) HNMR (110 KB) RP-HPLC (234 KB) MS (70 KB)

Handling Instructions (2659 KB)

References

[1]. Ide T. Enzymatic-HPLC method to analyze D-3-hydroxybutyric acid in rat serum. Biosci Biotechnol Biochem. 2010;74(8):1578-82. [Content Brief]

[2]. Mateusz Biernacki, et al. Production of (R)-3-hydroxybutyric acid by Arxula adeninivorans. AMB Express. 2017 Dec;7(1):4. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	7.9308 mL	39.6542 mL	79.3084 mL	198.2711 mL
	5 mM	1.5862 mL	7.9308 mL	15.8617 mL	39.6542 mL
	10 mM	0.7931 mL	3.9654 mL	7.9308 mL	19.8271 mL
	15 mM	0.5287 mL	2.6436 mL	5.2872 mL	13.2181 mL
	20 mM	0.3965 mL	1.9827 mL	3.9654 mL	9.9136 mL
	25 mM	0.3172 mL	1.5862 mL	3.1723 mL	7.9308 mL
	30 mM	0.2644 mL	1.3218 mL	2.6436 mL	6.6090 mL
	40 mM	0.1983 mL	0.9914 mL	1.9827 mL	4.9568 mL
	50 mM	0.1586 mL	0.7931 mL	1.5862 mL	3.9654 mL
	60 mM	0.1322 mL	0.6609 mL	1.3218 mL	3.3045 mL
	80 mM	0.0991 mL	0.4957 mL	0.9914 mL	2.4784 mL
	100 mM	0.0793 mL	0.3965 mL	0.7931 mL	1.9827 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

(R)-3-Hydroxybutanoic acid sodium Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(R)-3-Hydroxybutanoic acid13613-65-5(R)-(-)-3-Hydroxybutanoic acid(R)-3-Hydroxybutyric acidEndogenous MetaboliteendogenousmetaboliteacetoaceticvitaminsantibioticspheromonesInhibitorinhibitorinhibit

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(R)-3-Hydroxybutanoic acid sodium (Synonyms: (R)-(-)-3-Hydroxybutanoic acid sodium; (R)-3-Hydroxybutyric acid sodium)

Customer Review

Other Forms of (R)-3-Hydroxybutanoic acid sodium:

(R)-3-Hydroxybutanoic acid sodium Related Antibodies

6 Publications Citing Use of MCE (R)-3-Hydroxybutanoic acid sodium

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

(R)-3-Hydroxybutanoic acid sodium Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

(R)-3-Hydroxybutanoic acid sodium Related Classifications

Keywords:

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