Ractopamine hydrochloride
Based on 1 Customer Validation
Ractopamine hydrochloride (LY031537) is a potent and orally active β-adrenergic receptor (βAR) agonist with Kd value of ~25 nM for pig β1AR and β2AR. Ractopamine hydrochloride also is a mTAAR1 agonist with an EC50 of 16 μM. Ractopamine hydrochloride promotes muscle mass development, limits fat deposition, reduces feed consumption, increases total cellular protein synthesis, and improves growth rate and feed efficiency in finishing swine. Ractopamine hydrochloride can be used for researching to increase lean tissue growth and improve production efficiency in pigs.
For research use only. We do not sell to patients.
- Purity: 99.2%
- CAS No.: 90274-24-1
- Formula: C18H24ClNO3
- Molecular Weight:337.84
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
All Adrenergic Receptor Isoforms
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Biological Activity
Ractopamine (50 nM-100 μM) hydrochloride acts as a full agonist of mouse trace amine-associated receptor 1 (mTAAR1) in Xenopus laevis oocytes coexpressing hCFTR and mTAAR1, with an EC50 of 16 μM, via a cAMP-dependent pathway[1].
Ractopamine hydrochloride increases protein accumulation and synthesis in postfusion ELC5 myotubes in a concentration-dependent manner[3].
Ractopamine hydrochloride shows Kd value of ~25 nM for pig β1AR and β2AR[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Ractopamine (5-20 mg/kg; p.o.; daily; 48 days) hydrochloride administered in the diet of finishing swine linearly improves average daily gain, increases carcass weight and loin eye are[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:crossbred Camborough 25 × AGPIC 337 (170 barrows, 170 gilts, average initial body weight 107.3 kg)[2]
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Dosage:5 mg/kg; 10 mg/kg
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Administration:oral; divided into 3 daily portions; 28 days
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Result:Increased the average percentage of pigs nosing pen mates to 6.73% (vs control 5.62%) at 5 mg/kg.
Reduced the average percentage of pigs playing to 0.06% (vs control 0.26%) at 5 mg/kg.
Increased the average percentage of pigs drinking water to 2.06% (vs control 1.58%) at 5 mg/kg.
Increased the average percentage of pigs standing to 1.39% (vs control 0.85%) at 10 mg/kg.
Increased serum CK-MB concentration to 4361 U/L (vs control 2341 U/L) and serum CK-total concentration to 12,436 U/L (vs control 5811 U/L) at 5 mg/kg.
Increased serum CK-MB concentration to 4510 U/L (vs control 2341 U/L) and serum CK-total concentration to 10,707 U/L (vs control 5811 U/L) at 10 mg/kg.
Showed no significant effect on overall pig behavior classification, skin/carcass lesions, blood cortisol or lactate concentrations.
Chemical Information
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CAS No. 90274-24-1
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Appearance Solid
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Molecular Weight 337.84
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Formula C18H24ClNO3
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Color White to off-white
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SMILES
OC(CNC(C)CCC1=CC=C(O)C=C1)C2=CC=C(O)C=C2.Cl
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Synonyms
LY031537
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (296.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.40 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.40 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Liu X, et al. Ractopamine, a livestock feed additive, is a full agonist at trace amine-associated receptor 1. J Pharmacol Exp Ther. 2014;350(1):124-129. [Content Brief]
[2]. Athayde NB, et al. Stress susceptibility in pigs supplemented with ractopamine. J Anim Sci. 2013;91(9):4180-4187. [Content Brief]
[3]. Anderson PT, et al. Ractopamine increases total and myofibrillar protein synthesis in cultured rat myotubes. J Nutr. 1990;120(12):1677-1683. [Content Brief]
[5]. Stites CR, et al. The effect of ractopamine hydrochloride on the carcass cutting yields of finishing swine. J Anim Sci. 1991;69(8):3094-3101. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9600 mL | 14.7999 mL | 29.5998 mL | 73.9995 mL |
| 5 mM | 0.5920 mL | 2.9600 mL | 5.9200 mL | 14.7999 mL | |
| 10 mM | 0.2960 mL | 1.4800 mL | 2.9600 mL | 7.4000 mL | |
| 15 mM | 0.1973 mL | 0.9867 mL | 1.9733 mL | 4.9333 mL | |
| 20 mM | 0.1480 mL | 0.7400 mL | 1.4800 mL | 3.7000 mL | |
| 25 mM | 0.1184 mL | 0.5920 mL | 1.1840 mL | 2.9600 mL | |
| 30 mM | 0.0987 mL | 0.4933 mL | 0.9867 mL | 2.4667 mL | |
| 40 mM | 0.0740 mL | 0.3700 mL | 0.7400 mL | 1.8500 mL | |
| 50 mM | 0.0592 mL | 0.2960 mL | 0.5920 mL | 1.4800 mL | |
| 60 mM | 0.0493 mL | 0.2467 mL | 0.4933 mL | 1.2333 mL | |
| 80 mM | 0.0370 mL | 0.1850 mL | 0.3700 mL | 0.9250 mL | |
| 100 mM | 0.0296 mL | 0.1480 mL | 0.2960 mL | 0.7400 mL |