RAF1 Protein, Human (His)
Based on 1 Customer Validation
RAF1 is a kinase that links Ras GTPases to the MAPK/ERK cascade, influencing cell fate decisions. Activated RAF1 initiates the MAPK cascade and phosphorylates MEK1/2 and ERK1/2. RAF1, Human (His) is the recombinant human-derived RAF1 protein, expressed by E. coli, with labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
RAF1 is a kinase that links Ras GTPases to the MAPK/ERK cascade, influencing cell fate decisions. Activated RAF1 initiates the MAPK cascade and phosphorylates MEK1/2 and ERK1/2. RAF1, Human (His) is the recombinant human-derived RAF1 protein, expressed by E. coli, with labeled tag.
RAF1, a serine/threonine-protein kinase, serves as a pivotal regulatory link connecting membrane-associated Ras GTPases to the MAPK/ERK cascade, playing a decisive role in determining cellular fate decisions such as proliferation, differentiation, apoptosis, survival, and oncogenic transformation. Upon activation, RAF1 initiates a MAPK cascade involving the sequential phosphorylation of dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1, facilitated by PAK1, phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75' and adenylyl cyclases (ADCY2, ADCY5, and ADCY6), activating them. Additionally, RAF1 phosphorylates PPP1R12A, inhibiting its phosphatase activity. Beyond its role in the MAPK cascade, RAF1 can activate NF-kB and inhibit key signaling molecules involved in motility, apoptosis, proliferation, and angiogenesis. It exerts anti-apoptotic effects by translocating to the mitochondria, binding BCL2, and displacing BAD/Bcl2-antagonist of cell death. Furthermore, RAF1 regulates Rho signaling, migration, wound healing, and is implicated in oncogenic transformation by repressing the tight junction protein occludin (OCLN). Notably, RAF1 restrains caspase activation in response to specific stimuli, including Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
The kinase activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species Human
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Source E. coli
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Tag C-6*His
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Accession
P04049-1 (M1-F648)
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Gene ID5894
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Protein Length
Full Length
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Synonyms
RAF1; Raf Proto-Oncogene Serine/Threonine Protein Kinase; Raf-1 Proto-Oncogene, Serine/Threonine Kinase; Non-Specific Serine/Threonine Protein Kinase; Raf-1; Eosinophil Cationic Protein; RAF Proto-Oncogene Serine/Threonine-Protein Kinase; Ribonuclease 3;
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AA Sequence
MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF
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Predicted Molecular Mass
80 kDa
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Molecular Weight
Approximately 80 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0.
2.Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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