RFRP-3(human)
Based on 1 Customer Validation
RFRP-3 (Neuropeptide VF(124-131))(human), a human GnIH peptide homolog, is a potent inhibitor of gonadotropin secretion by inhibiting Ca2+ mobilization. RFRP-3(human) is a NPFF1 receptor agonist, it inhibits forskolin-induced production of cAMP with an IC50 of 0.7 nM.
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- Reinheit: 99.43%
- CAS. Nr.: 311309-27-0
- Formel: C45H72N14O10
- Molecular Weight:969.14
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Speicherung:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Biologische Aktivität
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| COS-1 | EC50 |
1.7 nM
Compound: NPVF, VPNLPQRF-NH2
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Agonist activity at human NPFF1 receptor expressed in COS1 cells assessed as [3H]inositol phosphate accumulation after 2 hrs by liquid scintillation counting
Agonist activity at human NPFF1 receptor expressed in COS1 cells assessed as [3H]inositol phosphate accumulation after 2 hrs by liquid scintillation counting
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[PMID: 22708927] |
| COS-1 | EC50 |
1.7 nM
Compound: NPVF, VPNLPQRF-NH2
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Agonist activity at human NPFF2 receptor expressed in COS1 cells assessed as [3H]inositol phosphate accumulation after 2 hrs by liquid scintillation counting
Agonist activity at human NPFF2 receptor expressed in COS1 cells assessed as [3H]inositol phosphate accumulation after 2 hrs by liquid scintillation counting
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[PMID: 22708927] |
RFRP-3 efficiently inhibits forskolin-induced production of cAMP with an IC50 of 0.7 nM[1]. Scatchard-plot analysis shows that 125I-labelled hRFRP-3 has a single class of high-affinity binding sites for the membrane fractions of CHO cells expressing rat OT7T022, the Kd value and the Bmax values are 0.19 nM and 1.3 pM, respectively.RFRP-3 specifically stimulate cells transfected with a new orphan 7TMR, OT7T022, it binds to OT7T022 as a specific ligand with high affinity (Kd= 0.19 nM)[1].RFRP-3 (10-8 to 10-14M) has no effect on LH and FSH levels alone, but when it combines with GnRH, LH and FSH secretion is significantly reduced by the combination[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 311309-27-0
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Appearance Solid
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Molecular Weight 969.14
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Formel C45H72N14O10
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Color White to off-white
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Synonyms
Neuropeptide VF(124-131)(human); Neuropeptide NPVF (human)
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Sequence Shortening
VPNLPQRF-NH2
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Lösungsmittel & Löslichkeit
DMSO : ≥ 100 mg/mL (103.18 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : ≥ 50 mg/mL (51.59 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: ≥ 50 mg/mL (51.59 mM); Clear solution
Reinheit & Dokumentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.0318 mL | 5.1592 mL | 10.3184 mL | 25.7961 mL |
| 5 mM | 0.2064 mL | 1.0318 mL | 2.0637 mL | 5.1592 mL | |
| 10 mM | 0.1032 mL | 0.5159 mL | 1.0318 mL | 2.5796 mL | |
| 15 mM | 0.0688 mL | 0.3439 mL | 0.6879 mL | 1.7197 mL | |
| 20 mM | 0.0516 mL | 0.2580 mL | 0.5159 mL | 1.2898 mL | |
| 25 mM | 0.0413 mL | 0.2064 mL | 0.4127 mL | 1.0318 mL | |
| 30 mM | 0.0344 mL | 0.1720 mL | 0.3439 mL | 0.8599 mL | |
| 40 mM | 0.0258 mL | 0.1290 mL | 0.2580 mL | 0.6449 mL | |
| 50 mM | 0.0206 mL | 0.1032 mL | 0.2064 mL | 0.5159 mL | |
| DMSO | 60 mM | 0.0172 mL | 0.0860 mL | 0.1720 mL | 0.4299 mL |
| 80 mM | 0.0129 mL | 0.0645 mL | 0.1290 mL | 0.3225 mL | |
| 100 mM | 0.0103 mL | 0.0516 mL | 0.1032 mL | 0.2580 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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