Ropinirole (Synonyms: SKF 101468)

Name: Ropinirole
Brand: MedChemExpress
SKU: HY-B0623
Rating: 5.0 (3 reviews)

Cat. No.: HY-B0623 Purity: 99.42%: Data Sheet
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Ropinirole (SKF 101468) is an orally active, potent D₃/D₂ receptor agonist with a K_i of 29 nM for D₂ receptor. Ropinirole has pEC₅₀s of 7.4, 8.4 and 6.8 for hD₂, hD₃ and hD₄ receptors, respectively. Ropinirole has no affinity for the D₁ receptors. Ropinirole has the potential for Parkinson's disease.

For research use only. We do not sell to patients.

Ropinirole Chemical Structure

CAS No. : 91374-21-9

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
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500 mg	Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Ropinirole:

3 Publications Citing Use of MCE Ropinirole

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Dopamine Receptor D₁ Receptor D₂ Receptor D₃ Receptor D₄ Receptor D₅ Receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]^[2]

D₂ Receptor

29 nM (Ki)

hD₂ Receptor

7.4 (pEC₅₀)

hD₃ Receptor

8.4 (pEC₅₀)

hD_4.4 Receptor

6.8 (pEC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
AtT20	EC₅₀	19.2 nM Compound: ropinirole	Agonist activity at human dopamine D3 receptor expressed in mouse AtT-20 cells assessed as stimulation of [35S]GTPgamma binding Agonist activity at human dopamine D3 receptor expressed in mouse AtT-20 cells assessed as stimulation of [35S]GTPgamma binding	[PMID: 20038106]
CHO	EC₅₀	10.3 nM Compound: Ropinirole	Agonist activity at human dopamine D3 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding Agonist activity at human dopamine D3 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding	[PMID: 20605099]
CHO	EC₅₀	10.3 nM Compound: ropinirole	Agonist activity at human dopamine D3 receptor expressed in CHO cells by [35S]GTPgammaS binding assay Agonist activity at human dopamine D3 receptor expressed in CHO cells by [35S]GTPgammaS binding assay	[PMID: 20146482]
CHO	EC₅₀	304 nM Compound: Ropinirole	Agonist activity at human dopamine D2 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding Agonist activity at human dopamine D2 receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding	[PMID: 20605099]
CHO	EC₅₀	304 nM Compound: Ropinirole	Agonist activity at human dopamine D2L receptor expressed in CHO cell membranes incubated for 1 hr by [35S]GTPgammaS binding assay Agonist activity at human dopamine D2L receptor expressed in CHO cell membranes incubated for 1 hr by [35S]GTPgammaS binding assay	[PMID: 25490054]
CHO	EC₅₀	304 nM Compound: ropinirole	Agonist activity at human dopamine D2L receptor expressed in CHO cells assessed as stimulation of [35S]GTPgamma binding Agonist activity at human dopamine D2L receptor expressed in CHO cells assessed as stimulation of [35S]GTPgamma binding	[PMID: 20038106]
CHO	EC₅₀	304 nM Compound: ropinirole	Agonist activity at human dopamine D2 receptor expressed in CHO cells by [35S]GTPgammaS binding assay Agonist activity at human dopamine D2 receptor expressed in CHO cells by [35S]GTPgammaS binding assay	[PMID: 20146482]
CHO	EC₅₀	4 nM Compound: ropinerole	Agonist activity at human recombinant dopamine D3 receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP accumulation Agonist activity at human recombinant dopamine D3 receptor expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP accumulation	[PMID: 17976986]
COS-7	EC₅₀	3.89 nM Compound: Ropinirole	Agonist activity at Rattus norvegicus (rat) dopamine D2/D3 receptor transfected in african green monkey COS7 cells assessed as inhibition of forskolin-stimulated adenylyl cyclase activity after 10 min Agonist activity at Rattus norvegicus (rat) dopamine D2/D3 receptor transfected in african green monkey COS7 cells assessed as inhibition of forskolin-stimulated adenylyl cyclase activity after 10 min	10.1007/s00044-004-0006-x
COS-7	EC₅₀	75.5 nM Compound: Ropinirole	Agonist activity at Rattus norvegicus (rat) dopamine D2 receptor transfected in african green monkey COS7 cells assessed as inhibition of forskolin-stimulated adenylyl cyclase activity after 10 min Agonist activity at Rattus norvegicus (rat) dopamine D2 receptor transfected in african green monkey COS7 cells assessed as inhibition of forskolin-stimulated adenylyl cyclase activity after 10 min	10.1007/s00044-004-0006-x
COS-7	IC₅₀	107 nM Compound: Ropinirole	Displacement of [3H]nemonapride from Rattus norvegicus (rat) wild type dopamine D3 receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting Displacement of [3H]nemonapride from Rattus norvegicus (rat) wild type dopamine D3 receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting	10.1007/s00044-004-0006-x
COS-7	IC₅₀	1803 nM Compound: Ropinirole	Displacement of [3H]nemonapride from Rattus norvegicus (rat) wild type dopamine D2 receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting Displacement of [3H]nemonapride from Rattus norvegicus (rat) wild type dopamine D2 receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting	10.1007/s00044-004-0006-x
COS-7	IC₅₀	56.1 nM Compound: Ropinirole	Displacement of [3H]nemonapride from Rattus norvegicus (rat) chimeric dopamine D3 trunk/D2 tail receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting Displacement of [3H]nemonapride from Rattus norvegicus (rat) chimeric dopamine D3 trunk/D2 tail receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting	10.1007/s00044-004-0006-x
COS-7	IC₅₀	985 nM Compound: Ropinirole	Displacement of [3H]nemonapride from Rattus norvegicus (rat) chimeric dopamine D2 trunk/D3 tail receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting Displacement of [3H]nemonapride from Rattus norvegicus (rat) chimeric dopamine D2 trunk/D3 tail receptor transfected in african green monkey COS7 cells after 1 hr by beta scintillation counting	10.1007/s00044-004-0006-x
HEK-293T	EC₅₀	30 nM Compound: Ropinirole	Partial agonist activity at human D2SR expressed in HEK293T cells co-expressing (EA)beta-arrestin2 and GRK2 assessed as induction of beta-arrestin2 recruitment after 5 hrs by chemiluminescence assay Partial agonist activity at human D2SR expressed in HEK293T cells co-expressing (EA)beta-arrestin2 and GRK2 assessed as induction of beta-arrestin2 recruitment after 5 hrs by chemiluminescence assay	[PMID: 28489379]
HEK-293T	EC₅₀	330 nM Compound: Ropinirole	Agonist activity at human D2S receptor expressed in HEK293T cell membranes coexpressing Galphao1 assessed as induction of nucleotide exchange preincubated for 30 mins followed by addition of [35S]GTPgammaS measured after 30 mins by [35S]GTPgammaS binding assay Agonist activity at human D2S receptor expressed in HEK293T cell membranes coexpressing Galphao1 assessed as induction of nucleotide exchange preincubated for 30 mins followed by addition of [35S]GTPgammaS measured after 30 mins by [35S]GTPgammaS binding assay	[PMID: 28489379]
HEK-293T	EC₅₀	89 nM Compound: Ropinirole	Partial agonist activity at human D2SR expressed in HEK293T cells co-expressing (EA)beta-arrestin2 assessed as induction of beta-arrestin2 recruitment after 5 hrs by chemiluminescence assay Partial agonist activity at human D2SR expressed in HEK293T cells co-expressing (EA)beta-arrestin2 assessed as induction of beta-arrestin2 recruitment after 5 hrs by chemiluminescence assay	[PMID: 28489379]
HEK293	EC₅₀	330 nM Compound: 2	Agonist activity at human D2SR expressed in HEK293 cell membranes co-expressing PTX insensitive variant of Galphao1 incubated for 30 mins by [35S]GTP-gammaS binding assay Agonist activity at human D2SR expressed in HEK293 cell membranes co-expressing PTX insensitive variant of Galphao1 incubated for 30 mins by [35S]GTP-gammaS binding assay	[PMID: 28870802]
HEK293	EC₅₀	89 nM Compound: 2	Agonist activity at human D2SR expressed in HEK293 cells incubated for 5 hrs by beta-Arrestin 2 recruitment assay Agonist activity at human D2SR expressed in HEK293 cells incubated for 5 hrs by beta-Arrestin 2 recruitment assay	[PMID: 28870802]
Pituitary gland cell	EC₅₀	83 nM Compound: ropinerole	Agonist activity at human recombinant dopamine D2 receptor expressed in rat pituitary cells assessed as inhibition of forskolin-stimulated cAMP accumulation Agonist activity at human recombinant dopamine D2 receptor expressed in rat pituitary cells assessed as inhibition of forskolin-stimulated cAMP accumulation	[PMID: 17976986]

In Vitro

Ropinirole has affinity for D₃ receptors of 10-20 fold higher than the D₂ and D₄ receptors. Ropinirole is weakly active at alpha 2-adrenoceptors and 5-HT₂ receptors but inactive at 5-HT₁, benzodiazepine and gamma-aminobutyric acid receptors or alpha 1 and beta-adrenoceptors^[1]^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Ropinirole (0.1-10 mg/kg; i.p.) decreases intracranial self-stimulation (ICSS) thresholds and induces anxiolytic- and antidepressive-like effects without affecting motor activity or spatial memory^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

260.37

Formula

C₁₆H₂₄N₂O

CAS No.

91374-21-9

Appearance

Solid

Color

Pale purple to light pink

SMILES

O=C1NC2=C(C(CCN(CCC)CCC)=CC=C2)C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (192.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8407 mL	19.2034 mL	38.4069 mL
5 mM	0.7681 mL	3.8407 mL	7.6814 mL
10 mM	0.3841 mL	1.9203 mL	3.8407 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.42%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (246 KB) HNMR (245 KB) LCMS (325 KB)

Handling Instructions (2659 KB)

References

[1]. Eden, R.J., et al., Preclinical pharmacology of ropinirole (SK&F 101468-A) a novel dopamine D2 agonist. Pharmacol Biochem Behav, 1991. 38(1): p. 147-54. [Content Brief]

[2]. Mavrikaki M, et al. Ropinirole regulates emotionality and neuronal activity markers in the limbic forebrain. Int J Neuropsychopharmacol. 2014 Dec;17(12):1981-93. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.8407 mL	19.2034 mL	38.4069 mL	96.0172 mL
	5 mM	0.7681 mL	3.8407 mL	7.6814 mL	19.2034 mL
	10 mM	0.3841 mL	1.9203 mL	3.8407 mL	9.6017 mL
	15 mM	0.2560 mL	1.2802 mL	2.5605 mL	6.4011 mL
	20 mM	0.1920 mL	0.9602 mL	1.9203 mL	4.8009 mL
	25 mM	0.1536 mL	0.7681 mL	1.5363 mL	3.8407 mL
	30 mM	0.1280 mL	0.6401 mL	1.2802 mL	3.2006 mL
	40 mM	0.0960 mL	0.4801 mL	0.9602 mL	2.4004 mL
	50 mM	0.0768 mL	0.3841 mL	0.7681 mL	1.9203 mL
	60 mM	0.0640 mL	0.3201 mL	0.6401 mL	1.6003 mL
	80 mM	0.0480 mL	0.2400 mL	0.4801 mL	1.2002 mL
	100 mM	0.0384 mL	0.1920 mL	0.3841 mL	0.9602 mL

Ropinirole Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ropinirole91374-21-9SKF 101468SKF101468SKF-101468Dopamine ReceptorD3D2D1D4Parkinson'sdiseaseInhibitorinhibitorinhibit

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Ropinirole (Synonyms: SKF 101468)

Customer Review

Other Forms of Ropinirole:

Ropinirole Related Antibodies

3 Publications Citing Use of MCE Ropinirole

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Dopamine Receptor Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Ropinirole Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Ropinirole Related Classifications

Keywords:

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