Schisantherin D
Based on 1 Customer Validation
Schisantherin D is a lignan. Schisantherin D can be isolated from Kadsura interior. Schisantherin D downregulates the expression of ETBR and inhibits the secretion of ECM and ET-1. Schisantherin D alleviates EtOH + ET-1-induced hepatocyte cytotoxicity. Schisantherin D potently inhibits HIV replication in cells.
For research use only. We do not sell to patients.
- Purity: 99.66%
- CAS No.: 64917-82-4
- Formula: C29H28O9
- Molecular Weight:520.53
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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ETB |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| H9 | EC50 |
0.5 μg/mL
Compound: 4
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Cytotoxicity against mock-infected human H9 cells after 4 days
Cytotoxicity against mock-infected human H9 cells after 4 days
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[PMID: 8946749] |
| H9 | IC50 |
25.3 μg/mL
Compound: 4
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Antiviral activity against HIV1 3B in human H9 cells assessed as inhibition of p24 antigen production after 4 days by ELISA
Antiviral activity against HIV1 3B in human H9 cells assessed as inhibition of p24 antigen production after 4 days by ELISA
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[PMID: 8946749] |
Schisantherin D (50 μM; 2 h) attenuates EtOH + ET-1-induced cytotoxicity and enhances cell viability in HL-7702 hepatocytes[1].
Schisantherin D inhibits the secretion of Col-I, Col-III, FN and ET-1 by LX-2 hepatic stellate cells stimulated with EtOH + ET-1[1].
Schisantherin D (0.8-100 µg/mL; 4 days) potently inhibits HIV replication in H9 lymphocytes, with an EC50 of 0.5 µg/mL, a therapeutic index of 50.6, and a cytotoxic IC50 of 25.3 µg/mL[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HL-7702 human liver parenchymal cells
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Concentration:50 μM
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Incubation Time:2 h pre-incubation, followed by 24 h co-incubation with EtOH + ET-1
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Result:Increased the viability of EtOH + ET-1-injured HL-7702 cells to 90.46%, compared to 77.60% viability in the untreated injury model group.
Showed no cytotoxicity alone, with 94.82% cell viability relative to untreated controls.
Chemical Information
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CAS No. 64917-82-4
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Appearance Solid
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Molecular Weight 520.53
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Formula C29H28O9
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Color White to light yellow
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SMILES
COC1=C(C([C@@H]([C@@](O)2C)OC(C3=CC=CC=C3)=O)=CC4=C1OCO4)C(C(C[C@@H]2C)=CC5=C6OCO5)=C6OC
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 100 mg/mL (192.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Xu JB, et al. Lignans from Schisandra chinensis ameliorate alcohol and CCl4-induced long-term liver injury and reduce hepatocellular degeneration via blocking ETBR. J Ethnopharmacol. 2020;258:112813. [Content Brief]
[2]. Chen DF, et al. Two new lignans, interiotherins A and B, as anti-HIV principles from Kadsura interior. J Nat Prod. 1996;59(11):1066-1068. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9211 mL | 9.6056 mL | 19.2112 mL | 48.0280 mL |
| 5 mM | 0.3842 mL | 1.9211 mL | 3.8422 mL | 9.6056 mL | |
| 10 mM | 0.1921 mL | 0.9606 mL | 1.9211 mL | 4.8028 mL | |
| 15 mM | 0.1281 mL | 0.6404 mL | 1.2807 mL | 3.2019 mL | |
| 20 mM | 0.0961 mL | 0.4803 mL | 0.9606 mL | 2.4014 mL | |
| 25 mM | 0.0768 mL | 0.3842 mL | 0.7684 mL | 1.9211 mL | |
| 30 mM | 0.0640 mL | 0.3202 mL | 0.6404 mL | 1.6009 mL | |
| 40 mM | 0.0480 mL | 0.2401 mL | 0.4803 mL | 1.2007 mL | |
| 50 mM | 0.0384 mL | 0.1921 mL | 0.3842 mL | 0.9606 mL | |
| 60 mM | 0.0320 mL | 0.1601 mL | 0.3202 mL | 0.8005 mL | |
| 80 mM | 0.0240 mL | 0.1201 mL | 0.2401 mL | 0.6003 mL | |
| 100 mM | 0.0192 mL | 0.0961 mL | 0.1921 mL | 0.4803 mL |
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