SF-3-030

SF-3-030 (2.5-60 μM; 30 min) selectively inhibits ERK2-mediated phosphorylation of the F-site-dependent Thr325 residue on c-Fos in vitro, with no effect on D-domain-containing substrates (RSK1) or p38α-mediated ATF2 phosphorylation^[2].
SF-3-030 (1-100 μM; 20 min-5 h) inhibits AP-1 and SRE promoter activity in EGF/PMA-stimulated HeLa/HEK293 cells and suppresses basal AP-1 activity in A375 melanoma cells, showing greater potency against PMA-induced responses than against EGF-induced activity^[2].
SF-3-030 (5-100 μM; 30 min-1 h) selectively inhibits EGF/PMA (HY-18739)-induced expression of F-site-containing IEGs (c-Fos, Fra1, FosB, FosB2, c-Myc) in HeLa and A375 cells, with minimal effect on non-F-site IEGs (c-Jun, Egr1)^[2].
SF-3-030 (1-100 μM; 24 h) selectively inhibits proliferation and induces apoptosis in melanoma cells (A375, RPMI7951) with constitutive ERK1/2 activity, with GI₅₀ values of 7.1 and 4.6 μM, respectively, while showing selectivity over Jurkat cells with a GI₅₀ > 50 μM)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.